Presidential Young Investigator Award: Studies in Biochemical Engineering

生物化学工程的研究主要集中在两个方面：组织培养反应器和制备型高效液相色谱法分离生物制品。正常哺乳动物细胞的大规模培养对疫苗、纤溶酶原激活剂、激素和各种多肽等高价值生物制品的生产具有重要意义。同时正在进行的是设计和评估一个5升的组织培养反应器的性能，该反应器采用传统和“液体”微载体的灌注系统。在生物分离器领域，本研究旨在通过提供一种系统的方法来评估各种色谱柱及其蛋白质分离能力，从而为高效液相色谱柱的设计和扩大规模做出贡献。

Biochemical engineering research is conducted in two areas: tissue-culture reactors and separation of biologicals by preparative high performance liquid chromatography (HPLC). Large-scale cultivation of normal mammalian cells is of importance to the production of very high value biologicals such as vaccines, plasminogen activators, hormones and various polypeptides. Also underway is the design and evaluation of the performance of a 5-liter tissue-culture reactor with a perfusion system using conventional and "liquid" microcarriers. In the area of bioseparators, this research effort is aiming to contribute to the design and scale-up of HPLC columns by providing a systematic approach in the evaluation of various columns and operations with respect to their protein separation capabilities.