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Transmission Electron Microscopy Service for SPARC Consortium Investigators

为 SPARC 联盟研究人员提供的透射电子显微镜服务

基本信息

批准号：
10348802
负责人：
LEIF A HAVTON
金额：
$ 24.65万
依托单位：
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-01 至 2023-03-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10348802
关键词：
Address Anatomy Animal Model Area Australia Autonomic ganglion Axon Back Biological Collaborations Custom Data Data Collection Development Device Designs Doctor of Philosophy Endocrine Family suidae Ferrets Florida Funding Ganglia Goals Heart Horns Human Hypoglossal nerve structure Image Immunoelectron Microscopy Investigation Knowledge Laboratories Lower urinary tract Mammals Modeling Mus Myelinated nerve fiber Nerve Nerve Tissue Nervous system structure Neurosecretory Systems Organ Oryctolagus cuniculus Pancreas Pelvis Peripheral Peripheral Nerves Plant Resins Process Protocols documentation Rattus Regulation Research Research Personnel Respiration Respiratory System Rodent Services Side Spinal Cord Structure Study models Synapses Synaptic Potentials Thinness Tissue Embedding Tissue Harvesting Tissue Preservation Tissues Translations Transmission Electron Microscopy Universities Visceral Work autonomic nerve blood glucose regulation connectome data resource functional outcomes gastrointestinal system gut-brain axis human tissue improved member model design myelination nerve supply neural circuit neuroregulation relating to nervous system sex tissue processing

项目摘要

Organ function may be modulated by stimulating peripheral activity and targeting, for instance, peripheral nerves and autonomic ganglia. However, the detailed functional and anatomical organization is not well understood for autonomic nerves, ganglia and organs involved with visceral functions. Significant knowledge gaps exist with regards to the neural control of many organ functions, including those of the heart, respiratory system, lower urinary tract, and gastro-intestinal system. An improved understanding of the various components of neural circuitries and innervation of peripheral organs is needed in order to provide improved modeling and design of devices to allow for optimal modulation of select organ functions. Transmission electron microscopy (TEM) allows for the identification of the fine structure of neural tissues to characterize, for instance, axonal composition and myelination, organization of neural circuitry in autonomic ganglia, and innervation of autonomic targets. The proposed studies will aim at establishing a TEM service for investigators supported by the Stimulating Peripheral Activity to Relieve Conditions (SPARC) consortium. A total of 6 SPARC-funded projects, which include 7 SPARC-affiliated investigator laboratories, with defined needs for TEM studies have been identified. The projects include studies on the autonomic control of the heart, brain-gut interactions, hypoglossal nerve-influences on respiration, lower urinary tract function, neuro-endocrine regulation of glucose, and modeling of vagal nerve functions. TEM studies will include detailed characterization of myelinated and unmyelinated axons in visceral and somatic nerves as well as organization of autonomic ganglia, and potential synaptic regulation of endocrine functions. Studies will be performed using rodent, large mammal, and human tissues to allow for interspecies comparisons. Sex as a biological variable will also be included in the studies. The TEM service will work closely with each participating SPARC laboratory and provide advice on tissue harvesting and preservation as well as perform tissue processing and embedding of tissues in plastic resin, semi-thin and ultrathin sectioning for ultrastructural studies, TEM of nervous tissues, pre- and post-embedding immuno-EM studies, quantitative analysis of nerves, ganglia, neural circuits, and synaptic structures, customization of TEM protocols to accommodate project specific needs, and interpretation of ultrastructure and quantitative TEM data. It is expected that the proposed TEM studies will add value to SPARC-funded investigations and augment interpretability of functional and modeling studies as well as provide critical ultrastructural data for an evidence-driven approach to the development of new and improved neuro-modulatory strategies.

器官功能可以通过刺激外周活动和靶向来调节，例如，

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A novel statistical methodology for quantifying the spatial arrangements of axons in peripheral nerves.

DOI：
10.3389/fnins.2023.1072779
发表时间：
2023
期刊：
FRONTIERS IN NEUROSCIENCE
影响因子：
4.3
作者：
Shemonti, Abida Sanjana;Plebani, Emanuele;Biscola, Natalia P.;Jaffey, Deborah M.;Havton, Leif A.;Keast, Janet R.;Pothen, Alex;Dundar, M. Murat;Powley, Terry L.;Rajwa, Bartek
通讯作者：
Rajwa, Bartek

High-throughput segmentation of unmyelinated axons by deep learning.

DOI：
10.1038/s41598-022-04854-3
发表时间：
2022-01-24
期刊：
Scientific reports
影响因子：
4.6
作者：
Plebani E;Biscola NP;Havton LA;Rajwa B;Shemonti AS;Jaffey D;Powley T;Keast JR;Lu KH;Dundar MM
通讯作者：
Dundar MM

Ultraflexible and Stretchable Intrafascicular Peripheral Nerve Recording Device with Axon-Dimension, Cuff-Less Microneedle Electrode Array.

DOI：
10.1002/smll.202200311
发表时间：
2022-05
期刊：
SMALL
影响因子：
13.3
作者：
Yan, Dongxiao;Jiman, Ahmad A.;Bottorff, Elizabeth C.;Patel, Paras R.;Meli, Dilara;Welle, Elissa J.;Ratze, David C.;Havton, Leif A.;Chestek, Cynthia A.;Kemp, Stephen W. P.;Bruns, Tim M.;Yoon, Euisik;Seymour, John P.
通讯作者：
Seymour, John P.

Human organ donor-derived vagus nerve biopsies allow for well-preserved ultrastructure and high-resolution mapping of myelinated and unmyelinated fibers.

DOI：
10.1038/s41598-021-03248-1
发表时间：
2021-12-13
期刊：
Scientific reports
影响因子：
4.6
作者：
Havton LA;Biscola NP;Stern E;Mihaylov PV;Kubal CA;Wo JM;Gupta A;Baronowsky E;Ward MP;Jaffey DM;Powley TL
通讯作者：
Powley TL

Computational modelling of nerve stimulation and recording with peripheral visceral neural interfaces.