Field Instrument for Assessment of Arsenic Exposure

砷暴露评估现场仪器

• 批准号: 10484041
• 负责人: Avni A Argun
• 金额: $ 84.51万
• 依托单位: GINER, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-09 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10484041
• 关键词: Acceleration; Acute; Area; Arsenic; Assessment tool; Biological Assay; Biological Markers; Blood; Body Fluids; Cadmium; Chronic; Clinical; Collaborations; Communities; Community Health; Complex; Computer software; Country; Coupled; Detection; Development; Disease; Electronics; Ensure; Environment; Environmental Exposure; Environmental Health; Environmental Pollutants; Epidemiologist; Epidemiology; Equipment; Evaluation; Exposure to; Food; Freezing; Goals; Health; Heavy Metals; High Pressure Liquid Chromatography; Human; Industrialization; Ingestion; Inhalation; Institution; International; Investments; Lab On A Chip; Laboratories; Lead; Letters; Liquid substance; Manganese; Mass Spectrum Analysis; Measurement; Measures; Medical; Metal exposure; Metals; Methods; Microfluidic Microchips; Microfluidics; Monitor; Outcome; Performance; Phase; Physiological; Plasma; Population; Populations at Risk; Prevalence; Public Health; Public Health Schools; Reagent; Research Personnel; Resolution; Risk; Safety; Sampling; Science; Scientist; Series; Site; Soil; Source; Specialist; System; Technology; Testing; Time; Toxic effect; Trace metal; Training; Transportation; Universities; Urine; Validation; Vial device; Water; Work; analytical method; bioaccumulation; commercialization; computerized data processing; contaminated drinking water; cost; cost effective; data quality; data tools; design; detection limit; detection method; diagnostic technologies; disorder prevention; drinking water; epidemiology study; experience; exposed human population; fabrication; field study; health science research; healthcare community; human subject; human tissue; improved; instrument; interest; medical schools; metal poisoning; next generation; operation; portability; portable monitoring; prevent; programs; prototype; rapid testing; real time monitoring; response; screening; sensor; sensor technology; skills; superfund site; tool; toxic metal; urinary; usability

Project Summary Human exposure to toxic heavy metals existing in drinking water, food, and the environment is an area of increasing national and international concern. Heavy metals are significant environmental pollutants because they tend to persist, bioaccumulate and can result in serious adverse acute or chronic health effects after ingested or inhaled. Traditionally, trace metals are measured from human tissues or fluids (blood or urine) in centralized laboratories using complex analytical methods such as high-performance liquid chromatography (HPLC) or inductively coupled plasma mass spectroscopy (ICP-MS). However, in view of the intensive labor, analytical costs and long turnaround times associated with centralized laboratory analyses, there is an immediate need for a portable and inexpensive system for on-site monitoring of human exposure to heavy metals. These rapid, field measurements of human exposure in the population would greatly help epidemiology researchers to conduct their studies and also help the regulatory institutions with better determination of safety limits and prevention of diseases caused by heavy metal exposures. An important goal of this Direct to Phase II proposal is to develop a technology that meet the technical and practical needs of environmental researchers, clinicians, and epidemiologists. The ultimate goal is to deliver a reliable field prototype of a sensing instrument for the current unmet needs for rapid testing of trace amounts of metals in urine samples, and to accelerate the commercialization of this product. In this Phase IIB study, Giner will develop and demonstrate the heavy metals monitor prototype from bench-top assay platform to a field-ready instrument with a portable size, with integrated components for assay operation, electronic units and software packages for data processing and user interface. Meanwhile Giner will develop and fabricate a series of disposable microfluidic lab-on-a-chip cartridge for convenient measurements of multiple metal(s) and metal combinations from large numbers of human subjects. The various cartridge designs will allow the monitor instrument to determine the presence and concentrations of multiple metals. The overall performances of the prototype will then be evaluated thoroughly through a large scale of field testing with four renowned epidemiology collaborators, as well as a team of user experience specialists, which would improve the sensing capabilities, tool reliability and usability towards commercialization. This proposed project will achieve a critical step towards the commercial release of a heavy metal field monitor, which has the potential to become a critical tool to support environmental and public health community for developing strategies to prevent toxic metals-related diseases. Giner is teaming up with leading commercial partners which specialize in the development and commercialization of screening and diagnostic technologies.

项目摘要 人类对饮用水、食物和环境中存在的有毒重金属的暴露是 日益受到国家和国际关注。重金属是重要的环境污染物，因为 它们往往会持续存在，生物积累，并可能导致严重的不良急性或慢性健康影响 吸入或吸入的。传统上，微量金属是从人体组织或液体(血液或尿液)中测量的。 使用高效液相色谱等复杂分析方法的集中式实验室 或电感耦合等离子体质谱(ICP-MS)。但是，鉴于劳动强度大， 与集中式实验室分析相关的分析成本和长时间周转时间，立竿见影 需要一种便携式和廉价的系统，用于现场监测人类接触重金属的情况。这些 对人群中人类暴露的快速现场测量将极大地帮助流行病学研究人员 进行研究，并帮助监管机构更好地确定安全限值和 预防因接触重金属而引起的疾病。 这一直接到第二阶段计划的一个重要目标是开发一种满足技术和 环境研究人员、临床医生和流行病学家的实际需求。最终目标是提供一个 可靠的现场传感仪器样机，可满足当前未满足的痕量快速检测需求 尿样中的金属含量，并加快该产品的商业化进程。在这项IIB期研究中，Giner 将开发和演示从台式化验平台到现场就绪的重金属监测仪原型 便携大小的仪器，带有用于分析操作的集成组件、电子单元和软件 用于数据处理和用户界面的包。与此同时，吉纳将开发和制造一系列 一次性微流控芯片实验室盒，方便测量多种金属(S)和金属 来自大量人类受试者的组合。各种墨盒设计将允许显示器 用于确定多种金属的存在和浓度的仪器。这两支队伍的整体表现 然后，原型将通过四个著名的流行病学的大规模现场测试进行彻底的评估 合作者以及用户体验专家团队，这将提高感知能力， 面向商业化的工具可靠性和可用性。 这个拟议的项目将朝着重金属现场监测器的商业发布迈出关键的一步， 它有可能成为支持环境和公共卫生社区的关键工具 制定预防有毒金属相关疾病的战略。Giner正在与领先的商业广告公司合作 专门从事筛查和诊断技术的开发和商业化的合作伙伴。