seqFISH core for in situ cell type identification
seqFISH core for in situ cell type identification
批准号:
10438690
负责人:
Long Cai
金额:
$37.27万
依托单位国家:
美国
项目类别:
财政年份:
2018
资助国家:
美国
项目状态:
已结题
起止时间:
2018-09-15 至 2024-06-30
关键词:
3-DimensionalBackBiochemistryBiological AssayBrainCalciumCell CommunicationCellsChickensComplementComplexComputer softwareDataData Science CoreData SetDetectionDevelopmentEmbryoEnvironmentFluorescent in Situ HybridizationGene ExpressionGene Expression ProfileGenesGenomic approachGenomicsGoalsHippocampus (Brain)HumanImageIn SituIn VitroInterneuronsLabelLocationMapsMeasurementMessenger RNAMethodsModelingMolecularMusNatureNeuronsPancreasPatternPhysiologicalPopulationProcessProtocols documentationRNAResearchResolutionResourcesSamplingSliceTechnologyTimeTissue SampleTissuesTranslatingV1 neuronValidationVisualWorkZebrafisharea striatabiological systemscell typeexperimental studyimaging studyin vivoin vivo calcium imaginginnovative technologiesinstrumentmultiplexed imagingrelating to nervous systemresponsesingle moleculesingle-cell RNA sequencingtool
中文摘要
项目摘要
这个核心的目标是系统地表征不同的分子身份
使用连接学和尖端技术相结合的皮质内神经元群
SeqFISH方法用于单细胞RNA的多重检测。蔡氏实验室最近
开发了一种使用顺序杂交和单分子FISH技术来多重
在复杂的组织样本中,大量的mRNA分子直接存在于单个细胞中。一个
哥伦比亚大学、加州大学伯克利分校和加州理工学院的协作团队将应用这项技术来识别
活体钙成像实验后小鼠脑内不同细胞类型的原位研究
以及体外连接性测量。
英文摘要
Project Summary
The goal of this core is to systematically characterize the molecular identities of distinct
neuronal populations within the cortex using a combination of connectomics and cutting edge
seqFISH methods to multiplex RNA detection in single cells. The Cai lab has recently
developed a technology using sequential hybridization and single molecule FISH to multiplex a
large number of mRNA molecules directly in single cells in complex tissue samples. A
collaborative team at Columbia, UC Berkeley and at Caltech will apply this technology to identify
distinct cell types in the mouse brain in situ following in vivo live calcium imaging experiments as
well as in vitro connectivity measurements.
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