seqFISH core for in situ cell type identification

用于原位细胞类型鉴定的 seqFISH 核心

• 批准号: 10438690
• 负责人: Long Cai
• 金额: $ 37.27万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-15 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10438690
• 关键词: 3-Dimensional; Back; Biochemistry; Biological Assay; Brain; Calcium; Cell Communication; Cells; Chickens; Complement; Complex; Computer software; Data; Data Science Core; Data Set; Detection; Development; Embryo; Environment; Fluorescent in Situ Hybridization; Gene Expression; Gene Expression Profile; Genes; Genomic approach; Genomics; Goals; Hippocampus (Brain); Human; Image; In Situ; In Vitro; Interneurons; Label; Location; Maps; Measurement; Messenger RNA; Methods; Modeling; Molecular; Mus; Nature; Neurons; Pancreas; Pattern; Physiological; Population; Process; Protocols documentation; RNA; Research; Resolution; Resources; Sampling; Slice; Technology; Time; Tissue Sample; Tissues; Translating; V1 neuron; Validation; Visual; Work; Zebrafish; area striata; biological systems; cell type; experimental study; imaging study; in vivo; in vivo calcium imaging; innovative technologies; instrument; multiplexed imaging; relating to nervous system; response; single molecule; single-cell RNA sequencing; tool

Project Summary The goal of this core is to systematically characterize the molecular identities of distinct neuronal populations within the cortex using a combination of connectomics and cutting edge seqFISH methods to multiplex RNA detection in single cells. The Cai lab has recently developed a technology using sequential hybridization and single molecule FISH to multiplex a large number of mRNA molecules directly in single cells in complex tissue samples. A collaborative team at Columbia, UC Berkeley and at Caltech will apply this technology to identify distinct cell types in the mouse brain in situ following in vivo live calcium imaging experiments as well as in vitro connectivity measurements.

项目摘要 这个核心的目标是系统地表征不同的分子身份， 使用连接组学和切割边缘的组合在皮层内的神经元群体 seqFISH方法在单细胞中进行多重RNA检测。Cai实验室最近 开发了一种使用顺序杂交和单分子FISH的技术， 在复杂组织样品中的单个细胞中直接存在大量mRNA分子。一 哥伦比亚大学、加州大学伯克利分校和加州理工学院的一个合作小组将应用这项技术来识别 在体内活钙成像实验后，小鼠脑中原位的不同细胞类型， 以及体外连接性测量。