seqFISH core for in situ cell type identification
seqFISH core for in situ cell type identification
批准号:
10438690
负责人:
Long Cai
金额:
$37.27万
依托单位国家:
美国
项目类别:
财政年份:
2018
资助国家:
美国
项目状态:
已结题
起止时间:
2018-09-15 至 2024-06-30
关键词:
3-DimensionalBackBiochemistryBiological AssayBrainCalciumCell CommunicationCellsChickensComplementComplexComputer softwareDataData Science CoreData SetDetectionDevelopmentEmbryoEnvironmentFluorescent in Situ HybridizationGene ExpressionGene Expression ProfileGenesGenomic approachGenomicsGoalsHippocampus (Brain)HumanImageIn SituIn VitroInterneuronsLabelLocationMapsMeasurementMessenger RNAMethodsModelingMolecularMusNatureNeuronsPancreasPatternPhysiologicalPopulationProcessProtocols documentationRNAResearchResolutionResourcesSamplingSliceTechnologyTimeTissue SampleTissuesTranslatingV1 neuronValidationVisualWorkZebrafisharea striatabiological systemscell typeexperimental studyimaging studyin vivoin vivo calcium imaginginnovative technologiesinstrumentmultiplexed imagingrelating to nervous systemresponsesingle moleculesingle-cell RNA sequencingtool
中文摘要
项目摘要
这个核心的目标是系统地表征不同的分子身份,
使用连接组学和切割边缘的组合在皮层内的神经元群体
seqFISH方法在单细胞中进行多重RNA检测。Cai实验室最近
开发了一种使用顺序杂交和单分子FISH的技术,
在复杂组织样品中的单个细胞中直接存在大量mRNA分子。一
哥伦比亚大学、加州大学伯克利分校和加州理工学院的一个合作小组将应用这项技术来识别
在体内活钙成像实验后,小鼠脑中原位的不同细胞类型,
以及体外连接性测量。
英文摘要
Project Summary
The goal of this core is to systematically characterize the molecular identities of distinct
neuronal populations within the cortex using a combination of connectomics and cutting edge
seqFISH methods to multiplex RNA detection in single cells. The Cai lab has recently
developed a technology using sequential hybridization and single molecule FISH to multiplex a
large number of mRNA molecules directly in single cells in complex tissue samples. A
collaborative team at Columbia, UC Berkeley and at Caltech will apply this technology to identify
distinct cell types in the mouse brain in situ following in vivo live calcium imaging experiments as
well as in vitro connectivity measurements.
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