Understanding the role of convergence at the retinogeniculate synapse

了解视网膜突触收敛的作用

• 批准号: 10471203
• 负责人: Takuma Sonoda
• 金额: $ 7.01万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10471203
• 关键词: Adult; Area; Behavior; Biological Models; Brain; Brain region; Cells; Chronic; Complex; Data; Dorsal; Electrophysiology (science); Environment; Eye; Family Felidae; Goals; Knowledge; Lateral Geniculate Body; Learning; Measures; Mediating; Modeling; Motion; Mus; Natural regeneration; Nervous system structure; Neurons; Neurosciences Research; Output; Pathway interactions; Perception; Photic Stimulation; Primates; Property; Research; Retina; Retinal Ganglion Cells; Rodent; Role; Route; Sensory; Signal Transduction; Stream; Synapses; Testing; Thalamic structure; Therapeutic; Training; Visual; Visual Pathways; Visual Perception; Visual system structure; Weight; Work; area striata; career; cell type; experience; experimental study; in vivo; information processing; insight; neural circuit; novel; optogenetics; preference; response; retinogeniculate; sight restoration; tool; visual information; visual processing

PROJECT SUMMARY Retinal ganglion cells (RGCs) are the output cells of the retina and consist of over 30 types that encode distinct features of the visual scene. These parallel information streams encoded by different RGC types are routed to the dorsal lateral geniculate nucleus (dLGN) before being transmitted to the primary visual cortex (V1) to mediate visual perception. While it was long thought that dLGN neurons simply relay information encoded by RGCs to V1, emerging evidence has demonstrated that there is more convergence of RGC inputs onto dLGN neurons than previously appreciated. This raises the question of how information encoded by RGCs is transformed in the dLGN before reaching V1. The overall goal of this proposal is to understand the functional role of retinal convergence in visual processing. Proposed experiments will involve manipulating the activity of specific RGC types to understand how information is integrated in dLGN neurons. This proposal encompass two aims: (1) To assess the contribution of specific RGC types to the response properties of dLGN neurons (2) To determine if visual experience can alter the response properties of dLGN neurons by changing the strength of RGC inputs. This work will provide important insights into how parallel information streams are integrated in the dLGN and have broad implications for understanding the function of convergence in sensory processing. In conducting these experiments, I will learn how to pair optogenetics with in vivo electrophysiological recordings to study the nervous system at the synaptic and circuit levels. Conceptually, these experiments will teach me how to investigate the dynamic properties of neural circuits. The proposed research will be combined with a tailored training plan that will prepare me for a successful independent research career studying the nervous system at different levels.

项目摘要 视网膜神经节细胞（RGC）是视网膜的输出细胞，由30多种类型组成，其编码不同的信号传导通路。 视觉场景的特点。这些由不同RGC类型编码的并行信息流被路由到 背外侧膝状体核（dLGN），然后被传输到初级视觉皮层（V1）， 视觉感知虽然长期以来一直认为dLGN神经元只是将RGCs编码的信息传递给 V1，新出现的证据表明，RGC输入到dLGN神经元上的收敛性更强 比以前欣赏。这就提出了一个问题，即由RGC编码的信息如何在 dLGN达到V1之前。 这个建议的总体目标是了解视网膜会聚在视觉处理中的功能作用。 拟议的实验将涉及操纵特定RGC类型的活动，以了解信息如何 整合在dLGN神经元中。该提案包含两个目标：（1）评估特定环境的贡献 （2）为了确定视觉体验是否可以改变dLGN神经元的反应特性， dLGN神经元的反应特性通过改变RGC输入的强度。这项工作将提供重要 深入了解并行信息流如何集成在dLGN中，并对以下方面产生广泛影响： 理解在感觉处理中的会聚功能。 在进行这些实验时，我将学习如何将光遗传学与体内电生理记录配对 在突触和回路水平上研究神经系统。从概念上讲，这些实验将教会我 如何研究神经回路的动态特性。这项研究将与一项 量身定制的培训计划，这将使我为一个成功的独立研究职业生涯做好准备， 系统在不同层次。