A Novel Semi-autonomous Surgeon-in-the-loop in situ Robotic Bioprinting System for Functional and Cosmetic Restoration of Volumetric Muscle Loss Injuries

一种新型半自主外科医生在环原位机器人生物打印系统，用于体积肌肉丢失损伤的功能和美容恢复

• 批准号: 10473273
• 负责人: Farshid Alambeigi
• 金额: $ 135.41万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10473273
• 关键词: 3-Dimensional; 3D Print; Abate; Address; Adhesions; Algorithms; Anatomic Surface; Anatomy; Biochemical; Biophysics; Bioreactors; Clinical; Computer Assisted; Cosmetics; Cues; Custom; Deposition; Engineering; Ensure; Failure; Feedback; Geometry; Goals; Human body; In Situ; In Vitro; Injury; Institutes; Intuition; Lead; Monitor; Motion; Muscle; Musculoskeletal; Natural regeneration; Operative Surgical Procedures; Organ; Patients; Printing; Procedures; Process; Resolution; Robotics; Safety; Signal Transduction; Surface; Surgeon; Surgical complication; Surgical sutures; System; Time; Tissue Engineering; Tissue constructs; Tissues; base; bioink; bioprinting; design; functional restoration; human tissue; hydrogel scaffold; implantation; improved; injured; innovation; instrument; loss of function; multidisciplinary; muscle engineering; musculoskeletal injury; novel; pain reduction; restoration; robotic system; success; tissue regeneration; volumetric muscle loss; wound

Summary/Abstract: Our long-goal is to develop an unprecedented semi-autonomous surgeon-in-the-loop surgical robotic system and complementary computer-assisted algorithms to enable an intuitive in situ robotic bioprinting of human tissues and organs. More specifically, using this extrusion-based bioprinting system, a surgeon can (i) first utilize a high-resolution three-dimensional (3D) point cloud camera to plan an arbitrary spatial printing geometry on the target anatomical surface, (ii) co-operate with a robotic system to manipulate a custom- designed bioprinting instrument to precisely follow the planned printing geometry, and (iii) perform an intuitive and precise deposition of engineered bioinks to make tissue constructs on the target anatomical surface, while (iv) directly control and monitor the printing process to ensure the safety and success of the procedure. The focus of this proposal is simultaneous functional and cosmetic restoration of large volumetric muscle loss (VML) injuries by utilizing a novel engineered bioink- developed by our collaborators at the Terasaki Institute of Biomedical Innovation, a complementary robotic bioprinting system, and intuitive computer- assisted algorithms. Severe musculoskeletal injuries can lead to VML, where extensive musculoskeletal damage and tissue loss result in permanent loss of function. In small-scale injuries or strains, muscle is capable of endogenous regeneration and complete functional restoration. However, this ability is abated in VML, where the native biophysical and biochemical signaling cues are no longer present to facilitate tissue regeneration. Current state- of-the-art in vitro tissue engineering VML treatment procedures suffer from various issues including (i) prolonged culturing period in bioreactors demanding functionality enhancement prior to implantation in the body; (ii) adhesion failure of in vitro 3D printed hydrogel scaffolds to the remnant muscle, whether injected, sutured, or placed into the wound; and (iii) inability to be printed precisely in irregular curved 3D surfaces of large VML injuries. It is our central hypothesis that the proposed semi-autonomous robotic bioprinting system can collectively address the mentioned limitations of the current state-of-the-art solutions by (i) reducing complexity, surgical time, and complications associated with current VML treatments, (ii) immediately delivering and in situ printing of appropriate bioinks to the target anatomy and utilizing the human body as a natural bioreactor to induce tissue maturation and function, and (iii) providing real-time feedback on the 3D bioprinted constructs as well as the surgeon’s and patient’s motions to ensure precision of the bioprinting procedure for simultaneous functional and cosmetic restoration of the injured muscle. The proposed project is multidisciplinary and bridges the current gap between the robotic surgery, tissue engineering, and bioprinting fields. The contribution is significant, high impact, and innovative and can revolutionize the current clinical paradigm.

总结/摘要： 我们的长期目标是开发一种前所未有的半自主外科医生在环手术机器人 系统和互补计算机辅助算法，以实现直观的原位机器人 人体组织和器官的生物打印。更具体地，使用这种基于挤出的生物打印系统， 外科医生可以（i）首先利用高分辨率三维（3D）点云相机来规划任意空间 在目标解剖表面上打印几何形状，（ii）与机器人系统合作以操纵定制的 设计的生物打印仪器，以精确地遵循计划的打印几何形状，和（iii）执行直观的 以及工程生物墨水的精确沉积，以在目标解剖表面上制造组织构造， (iv)直接控制和监控印刷过程，以确保过程的安全和成功。的 该建议的重点是同时恢复大体积肌肉的功能和美容 损失（VML）的伤害，利用一种新的工程生物墨水-由我们的合作者在寺崎开发 生物医学创新研究所，一个互补的机器人生物打印系统，和直观的计算机- 辅助算法 严重的肌肉骨骼损伤可导致VML，其中广泛的肌肉骨骼损伤和组织损失 导致永久性功能丧失。在小规模的损伤或应变，肌肉是能够内源性 再生和完全功能恢复。然而，这种能力在VML中减弱了， 生物物理和生物化学信号传导线索不再存在以促进组织再生。当前状态- 现有技术的体外组织工程VML治疗程序存在各种问题， 在植入体内之前在需要功能增强的生物反应器中的培养期;（ii） 体外3D打印水凝胶支架与残余肌肉的粘附失败，无论是注射，缝合，还是 放置到伤口中;以及（iii）不能在大VML的不规则弯曲3D表面中精确打印 受伤 我们的中心假设是，所提出的半自主机器人生物打印系统可以共同 通过（i）减少复杂性、手术复杂性、手术复杂性和手术复杂性， 时间，和并发症与目前的VML治疗，（ii）立即交付和原位打印 并利用人体作为天然生物反应器来诱导组织 成熟和功能，以及（iii）提供关于3D生物打印构建体以及生物打印构建体的实时反馈。 外科医生和患者的运动，以确保生物打印过程的精确性， 对受伤肌肉的美容修复。拟议的项目是多学科的，弥合了目前的差距。 机器人手术、组织工程和生物打印领域之间的联系。贡献很大，很高， 影响力和创新性，可以彻底改变目前的临床模式。