Probing the three-dimensional organization of enhancer-promoter communication

探索增强子-启动子沟通的三维组织

• 批准号: 10472691
• 负责人: Jailynn Alyse Harke
• 金额: $ 3.41万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-08 至 2023-09-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10472691
• 关键词: 3-Dimensional; Address; Adopted; Alleles; Architecture; Binding Sites; Biological Assay; Brain; Brain region; Cells; Communication; Complex; Congenital Abnormality; DNA; Data; Development; Elements; Embryo; Embryonic Development; Enhancers; Fluorescent in Situ Hybridization; Frequencies; Gene Expression; Gene Expression Profile; Gene Expression Regulation; Genetic Transcription; Genome; Goals; Heterogeneity; Hi-C; Human; Image; In Situ Hybridization; Individual; Knowledge; Label; Left; Ligation; Maps; Measures; Mediating; Microscopy; Modeling; Mus; Mutant Strains Mice; Organism; Output; Population; Positioning Attribute; Proteins; RNA; Regulator Genes; Regulatory Element; Resolution; SHH gene; Structure; System; Techniques; Technology; Testing; Tissue Preservation; Tissues; Transcript; Translating; base; brain tissue; chromosome conformation capture; cohesin; combinatorial; design; developmental disease; experimental study; imaging approach; in situ imaging; in vivo; mutant; preservation; promoter; spatiotemporal; three dimensional structure

PROJECT SUMMARY The goal of this proposal is to determine whether gene regulation is the cause or consequence of three- dimensional (3-D) genome organization. Enhancers are cis-regulatory elements that drive spatiotemporal gene expression from their target promoter. Disruption of enhancer-promoter (E-P) interactions can result in severe developmental disorders and congenital malformations. Enhancers typically communicate with their cognate promoter within 3-D features of genome folding called topologically associating domains (TADs). These features were originally characterized by proximity ligation sequencing techniques (ie: Hi-C). The depletion of two architectural proteins, either CTCF or cohesin, resulted in the dissolution of TADs by Hi-C; however, imaging-based approaches revealed that 3-D structures remained. Moreover, the effect of architectural protein depletion on gene expression was relatively mild, suggesting that E- P communication is robust to TAD dissemination. These perplexing findings have left the field of genome organization divided about the formation and function of TADs. One hypothesis posits that E-P interactions give rise to TAD structure. The other hypothesis is that architectural proteins form TADs in order to facilitate the E-P interactions within. I hypothesize that both gene regulatory elements and architectural proteins contribute to 3-D topology. I will test the contribution of each model in a unified system and defined developmental context. In order to retain in vivo spatiotemporal information at single-cell resolution, I will investigate the 3-D organization of the Sonic hedgehog (Shh) TAD in mouse embryonic brain tissue using a fluorescence in situ hybridization (FISH) approach. I designed small (10 kilobase) DNA-FISH probes to measure the physical distances between E-P elements. My preliminary data for one E-P pair, revealed both enhancer-dependent and enhancer-independent proximity in specific regions of the developing brain. While the enhancer-dependent proximity supports the model of active enhancers in mediating 3-D structure, I hypothesize that the enhancer- independent proximity is mediated by architectural proteins. In Aim 1, I will map all E-P interactions for the Shh locus using sequential DNA-FISH. I will then determine the contribution of enhancers and architectural proteins to the locus’ configuration by using mutants devoid of enhancers and specific CTCF binding sites, respectively. The experiments in Aim 2 will explore complex E-P communication of two redundant Shh enhancers. I will analyze the transcriptional output and spatial organization of the redundant enhancers and determine if these metrics are altered in the absence of the reciprocal enhancer. Taken together, the data from this proposal will create a paradigm for understanding how combinatorial gene regulation intersects with 3-D genome organization. Importantly, preserving the in vivo developmental context will be invaluable for translating how E-P miscommunication results in developmental disorders and disease.

项目总结