Pericyte Mechanisms in Traumatic Brain Injury

创伤性脑损伤中的周细胞机制

• 批准号: 10383154
• 负责人: Eng H. Lo
• 金额: $ 38.06万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-06-15 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10383154
• 关键词: Acute; Address; Astrocytes; Blood Vessels; Brain Concussion; Brain Injuries; Bromodeoxyuridine; Carbon Monoxide; Cell Culture Techniques; Cell Death; Cerebrovascular Disorders; Coculture Techniques; Data; Dependence; Electron Microscopy; Etiology; Event; Extracellular Matrix; Extravasation; Gases; Gene Expression; Heme; Homeostasis; Human; Hypoxia; In Vitro; Injury; Integrins; Knock-out; Knockout Mice; Knowledge; Label; Link; Maps; Mechanics; Mediating; Medicine; Modeling; Molecular; Mus; Nature; Neurological outcome; Neurons; Nitric Oxide; Nitric Oxide Pathway; Oxygenases; Pathway interactions; Pericytes; Pharmacology; Publishing; Recovery; Role; Signal Transduction; Small Interfering RNA; Source; Stretching; TBI treatment; Testing; Therapeutic; Transgenic Mice; Traumatic Brain Injury; Traumatic Brain Injury recovery; base; brain endothelial cell; controlled cortical impact; experimental study; gray matter; heme oxygenase-1; improved; improved outcome; in vivo; inhibitor; injured; loss of function; mouse model; mutant; nerve stem cell; neurogenesis; novel; novel therapeutics; oligodendrocyte precursor; optical imaging; precursor cell; response; spatiotemporal; vascular injury; white matter

Pericyte Mechanisms in Traumatic Brain injury Pericyte mechanisms are poorly understood in TBI. This is the major gap in knowledge that we seek to address. Our pilot data (some published in Choi et al, Nature Medicine 2016) suggest that (a) pericytes are widely damaged in mouse models of concussion or controlled cortical impact, (b) pericyte injury involves HIF- 1a signaling, (c) disruption of pericyte-neural stem cell (NSC) crosstalk perturbs neurogenesis and interferes with TBI recovery, (d) pericyte-NSC crosstalk may involve nitric oxide (NO) pathways, (e) pericytes may also communicate with oligodendrocyte precursor cells (OPCs), and (f) treatments with carbon monoxide (CO) that enhance heme oxygenase (HO-1) signaling may restore pericyte crosstalk and improve recovery after TBI. Based on these pilot data, we propose this overall hypothesis: TBI triggers HIF-1a-mediated injury to pericytes and disrupts pericyte-NSC-OPC crosstalk thus interfering with endogenous recovery. If true, this hypothesis may have translational significance, i.e. rescuing “help-me” signaling between pericytes, NSCs and OPCs may improve gray and white matter recovery after TBI. We will test this hypothesis in four integrated aims. In Aim 1, we investigate cellular mechanisms that allow pericytes to support NSCs and OPCs, and ask how HIF-1a-mediated pericyte injury disrupts these crosstalk mechanisms. In Aim 2, we test CO as a way to augment HO-1 signaling for protecting pericytes. In Aim 3, we dissect integrin and HIF mechanisms for pericytes, NSCs and OPCs in vivo using two TBI models (mild-to-moderate concussion and more severe controlled cortical impact). In Aim 4, will use the two mouse models of concussion and controlled cortical impact to test the utility of CO-HO-1 signaling as a therapeutic approach for restoring pericyte-NSC-OPC crosstalk and improving recovery after TBI. To assess causality in our pathways, we will conduct gain and loss- of-function experiments using cell culture, in vivo mouse models, pharmacologic inhibitors, dominant mutant constructs, siRNA and knockouts, optical imaging and long-term neurological outcomes. This project should define a novel mechanism wherein widespread injury to pericytes underlie not only acute vascular injury after TBI, but also disrupts pericyte-NSC-OPC crosstalk pathways. Our findings may provide a new conceptual framework for potentially targeting pericyte mechanisms after TBI.

创伤性脑损伤中的周细胞机制 在创伤性脑损伤中，周细胞机制知之甚少。这是我们寻求的知识上的主要差距 地址。我们的实验数据(一些发表在Choi等人的《自然医学2016》上)表明：(A)周细胞是 在脑震荡或受控皮质撞击的小鼠模型中广泛受损，(B)周细胞损伤涉及HIF- 1A信号，(C)干扰周细胞-神经干细胞(NSC)串扰神经发生和干扰 随着创伤性脑损伤的恢复，(D)周细胞-神经干细胞串扰可能涉及一氧化氮(NO)途径，(E)周细胞也可能 与少突胶质前体细胞(OPC)通信，以及(F)一氧化碳(CO)治疗， 增强血红素加氧酶(HO-1)信号转导可能恢复周细胞串扰，促进脑损伤后的恢复。 基于这些先导数据，我们提出了这样的总体假设：脑损伤触发了hif-1a介导的周细胞损伤。 并破坏周细胞-NSC-OPC串扰，从而干扰内源性恢复。如果是真的，这个假设 可能具有翻译意义，即拯救周细胞、神经干细胞和OPC之间的“帮助我”信号可能 改善颅脑损伤后灰质和白质的恢复。我们将在四个综合目标中检验这一假设。 在目标1中，我们研究了允许周细胞支持神经干细胞和OPC的细胞机制，并询问如何 HIF-1a介导的周细胞损伤破坏了这些串扰机制。在目标2中，我们测试CO作为一种 增强HO-1信号转导保护周细胞。在目标3中，我们剖析了整合素和缺氧诱导因子的机制 使用两种脑损伤模型(轻至中度脑震荡和更严重的脑震荡)在体的周细胞、神经干细胞和OPC 受控的皮质影响)。在目标4中，将使用脑震荡和大脑皮质受控两种小鼠模型 CO-HO-1信号通路在修复周细胞-NSC-OPC中的作用 串扰和改善脑损伤后的恢复。为了评估我们的道路上的因果关系，我们将进行得失- 使用细胞培养、活体小鼠模型、药物抑制剂、显性突变体的功能实验 构建、siRNA和敲除、光学成像和长期神经学结果。 这个项目应该定义一种新的机制，在这种机制中，周细胞的广泛损伤不仅是急性 颅脑损伤后血管损伤，但也扰乱周细胞-NSC-OPC串扰通路。我们的发现可能会提供一种 脑外伤后潜在靶向周细胞机制的新概念框架。