Histology and Imaging Core

组织学和成像核心

• 批准号: 10414548
• 负责人: DEREK C MOLLIVER
• 金额: $ 26.03万
• 依托单位: UNIVERSITY OF NEW ENGLAND
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10414548
• 关键词: Academia; Advisory Committees; Antibodies; Applications Grants; Award; Biological; Biomedical Research; Brain; Businesses; Caliber; Caring; Centers of Research Excellence; Collaborations; Communication; Communities; Computer software; Contract Services; Contracts; Data; Densitometry; Development; Ensure; Equipment; Evaluation; Extramural Activities; Faculty; Fee-for-Service Plans; Feedback; Fees; Fluorescence; Freezing; Funding; Goals; Grant; Histologic; Histology; Human Resources; Image; Image Analysis; Immunohistochemistry; Income; Industry; Institutes; Institution; Investments; Laboratories; Laboratory Personnel; Maine; Maintenance; Medical center; Methods; Microscope; Microtome - medical device; New England; Pain; Paraffin Embedding; Performance; Phase; Plastic Embedding; Procedures; Provider; Publications; Reproducibility; Research; Research Institute; Research Methodology; Research Personnel; Research Project Grants; Research Support; Resources; Role; Sampling; Scanning; Secure; Sensory; Service delivery model; Services; Slide; Stains; Strategic Planning; Stream; Students; System; Technical Expertise; Time; Tissue Embedding; Training; United States National Institutes of Health; Universities; Validation; Wages; Work; base; bone; cryostat; experience; experimental study; imaging capabilities; industry partner; instrumentation; outreach; programs; quantitative imaging; research facility; success; synergism; two photon microscopy; two-photon; voucher

Prior to the initiation of this COBRE, there were no research cores at the University of New England (UNE). Phase 1 and 2 COBRE support resulted in the development of the Histology and Imaging Core (HIC) into a critical resource for COBRE investigators and the broader research community at UNE and the region. The Core's histology instrumentation includes tissue embedding equipment, microtomes, vibratomes, cryostats and slide staining workstations. Imaging equipment includes brightfield, widefield fluorescence, slide-scanning, confocal and 2-photon microscopes. Technical services include tissue embedding, sectioning, conventional histology stains, immunohistochemistry, and antibody optimization and validation. In Phase 2, the HIC provided services to 38 investigators (15 UNE-COBRE, 8 UNE-non- COBRE, 10 in external academia and 5 in industry), and trained 175 laboratory personnel. Also in Phase 2, a fee-for- service model was initiated, and strong partnerships with other IDeA institutions at Brown University and Maine Medical Center Research Institute were developed that, in combination with internal users, have the potential to be long-term sustaining providers of Core income. Special care has been taken to ensure that the Core capabilities do not duplicate those offered by other COBRE- and INBRE-supported research cores. Instead, the Core has strategically specialized in unique services such the processing of brain samples, bone samples and marine-derived samples. In Phase 3, the HIC will maintain its current outstanding level of performance, critically assess its performance, continue to develop its personnel professionally, and hire an additional technician to keep pace with the needs of its broadening user base. The HIC will also undertake a rigorous strategic planning program under the direction of a UNE expert in small business strategy, with the goal of completing its transition to post-award sustainability. The HIC has secured critical additional institutional support, including guarantees of post-Phase 3 salary for key Core personnel, protected time for Directors, laboratory space, assistance with maintenance and service contracts, and an internally funded core voucher program and continuation of the UNE mini-grant program for those striving for extramural support. Through these efforts, the UNE HIC will continue to provide excellent critical research support to its many users, ensuring maximum utility to UNE and the broader research community in Phase 3 and beyond.

在这个科布雷项目启动之前，新英格兰大学(UNE)没有研究中心。第一阶段及 2科布雷的支持使组织和成像核心(HIC)发展成为 科布雷调查人员和UNE和该地区更广泛的研究社区。骨核的组织学仪器 包括组织包埋设备、显微切割机、振荡器、低温恒温器和玻片染色工作站。成象 设备包括明场显微镜、宽场荧光显微镜、载玻片扫描显微镜、共焦显微镜和双光子显微镜。技术 服务包括组织包埋、切片、常规组织学染色、免疫组织化学和抗体。 优化和验证。在第二阶段，HIC向38名调查员提供了服务(15名UNE-Cobre，8名UNE-NON 其中10名来自外部学术界，5名来自工业界)，并培训了175名实验室人员。同样在第二阶段，一项费用- 启动了服务模式，并与布朗大学和缅因州医学院的其他IDEA机构建立了牢固的合作伙伴关系 中心研究所开发的，与内部用户相结合，具有长期发展的潜力 维持核心收入的提供者。已特别注意确保核心功能不会重复 那些由Cobre和INBRE支持的其他研究核心提供的。取而代之的是，核心在战略上专门从事 提供独特的服务，如大脑样本、骨骼样本和海洋衍生样本的处理。在第三阶段，HIC将 保持其目前的卓越业绩水平，严格评估其业绩，继续发展其人员 并聘请一名额外的技术人员，以跟上其不断扩大的用户基础的需求。HIC将 还在UNE小型企业战略专家的指导下进行严格的战略规划计划， 完成向获奖后可持续性过渡的目标。HIC已经获得了关键的额外机构 支持，包括保证关键核心人员在第三阶段后的工资，主任受保护的时间，实验室 空间、维修和服务合同方面的协助以及内部供资的核心代用券方案和续订 为那些努力寻求校外支持的人提供的UNE迷你赠款计划。通过这些努力，UNHIC将 继续为许多用户提供出色的关键研究支持，确保最大限度地利用UNE和 第三阶段及以后更广泛的研究社区。