Histology and Imaging Core

组织学和成像核心

• 批准号: 10176518
• 负责人: DEREK C MOLLIVER
• 金额: $ 22.24万
• 依托单位: UNIVERSITY OF NEW ENGLAND
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-15 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10176518
• 关键词: Address; Advisory Committees; Antibodies; Applications Grants; Award; Biological; Calcium; Canada; Caring; Cell physiology; Cellular Morphology; Centers of Research Excellence; Cities; Committee Members; Communication; Communities; Confocal Microscopy; Contract Services; Contracts; Data; Development; Educational workshop; Ensure; Equipment; Evaluation; Fee-for-Service Plans; Feedback; Fees; Freezing; Funding; Future; Goals; Grant; Histologic; Histology; Human Resources; Image; Image Analysis; Immunohistochemistry; Institution; Laboratories; Laboratory Personnel; Maine; Maintenance; Methods; Microscope; Microscopy; Microtome - medical device; Modeling; New England; Noise; Optics; Pain; Paraffin Embedding; Performance; Phase; Phototoxicity; Pilot Projects; Procedures; Protocols documentation; Quebec; Reporting; Reproducibility; Research; Research Personnel; Research Support; Resources; Sampling; Sensory; Services; Signal Transduction; Stains; Stream; Structure; Surveys; Syncope; System; Technical Expertise; Techniques; Time; Tissue Embedding; Tissues; Training; United States National Institutes of Health; Universities; Validation; Work; base; cohort; cost; cryostat; in vivo imaging; industry partner; innovation; instrument; instrumentation; medical specialties; outreach; programs; research facility; satisfaction; success; summer program; tissue processing; training opportunity; two photon microscopy; two-photon

Phase 1 COBRE support resulted in the successful initiation of the Histology and Imaging Core and its development into a critical resource for COBRE investigators and the research community at the University of New England. The Core’s histology instruments include paraffin embedding and section processing equipment, as well as microtomes, vibratomes and cryostats. Imaging equipment includes bright-field, epifluorescent, stereologic and confocal microscopes. Technical services include immunohistochemistry and antibody optimization. In Phase I, the H&I Core provided services to 18 investigators (8 UNE-COBRE, 6 UNE- non-COBRE, 2 external contracts, 2 external COBRE), trained over 60 laboratory personnel, developed 11 new stains and optimized 60 antibodies. In Phase II, the Histology and Imaging Core will maintain existing capabilities and its current outstanding level of performance and expand those capabilities significantly while beginning to prepare for post-award sustainability. Proposed expansion includes the addition of antibody validation services and the acquisition of a two-photon microscope configured for in vivo imaging. The superior abilities of the two-photon system to penetrate tissues and detect faint signals with minimum noise and phototoxicity will enable COBRE investigators to develop innovation in their experimental approaches. These capabilities will have a regional impact, as such an instrument does not currently exist anywhere in southern Maine. Finally, the H&I Core will provide an ambitious program of professional development for Core and COBRE personnel. Critical training opportunities have been identified, including a summer program offered by External Advisory Committee Member Dr. Yves De Koninck’s Neurophotonics Centre at Laval University in nearby Quebec City, Canada. In order for the Core services to be sustained into the future, a fee-for-service plan will be implemented, and an extensive outreach effort will be conducted to broaden the user base. Special care has been taken to ensure that the Core capabilities do not duplicate those offered by other COBRE and INBRE supported research cores. Instead, the Core has strategically specialized in unique services such as a specialty in the processing of non-standard samples in a variety of research models. By these efforts, the UNE H&I Core will provide critical research support to its many users and ensure maximum utility to the broader research community in the State of Maine and neighboring IDeA states.

第一阶段的COBRE支持导致了组织学和成像核心及其成功启动