Histology and Imaging Core

第一阶段Cobre支持导致成功启动组织学和成像核心及其 发展成为科布雷研究人员和芝加哥大学研究界的关键资源 新英格兰。核心的组织学工具包括石蜡包埋和切片处理 设备，以及显微切割机、振动器和低温恒温器。成像设备包括明视场、 荧光显微镜、体视学显微镜和共聚焦显微镜。技术服务包括免疫组织化学和 抗体优化。在第一阶段，H&I核心向18名调查员提供了服务(8名UNE-Cobre，6名UNE-Cobre)。 非Cobre，2个外部合同，2个外部Cobre)，培训了60多名实验室人员，开发了11 新的菌株和优化的60种抗体。在第二阶段，组织和成像核心将保持现有的 功能及其当前卓越的性能水平，并显著扩展这些功能，同时 开始为获奖后的可持续性做准备。拟议的扩展包括添加抗体 验证服务和购买配置用于活体成像的双光子显微镜。《上级》 双光子系统穿透组织和检测微弱信号的能力，噪声和 光毒性将使Cobre研究人员能够在他们的实验方法上进行创新。这些 能力将产生区域影响，因为这样的工具目前在南部任何地方都不存在 缅因州。最后，H&I Core将为Core提供一个雄心勃勃的专业发展计划 科布雷人事部。已经确定了关键的培训机会，包括由 外部咨询委员会成员Yves de Koninck博士在拉瓦尔大学的神经光子学中心#年 附近的加拿大魁北克市。为了使核心服务持续到未来，按服务收费 将实施这一计划，并将开展广泛的外联工作，以扩大用户基础。 已特别注意确保核心功能不会与其他公司提供的功能重复 科布雷和INBRE资助了研究核心。取而代之的是，核心在战略上专门从事独特的 服务于专业加工各种非标样的各种研究模式。通过 这些努力，UNE H&I Core将为其众多用户提供关键的研究支持，并确保最大限度地 对缅因州和邻近的IDEA州更广泛的研究社区的效用。

Phase 1 COBRE support resulted in the successful initiation of the Histology and Imaging Core and its development into a critical resource for COBRE investigators and the research community at the University of New England. The Core’s histology instruments include paraffin embedding and section processing equipment, as well as microtomes, vibratomes and cryostats. Imaging equipment includes bright-field, epifluorescent, stereologic and confocal microscopes. Technical services include immunohistochemistry and antibody optimization. In Phase I, the H&I Core provided services to 18 investigators (8 UNE-COBRE, 6 UNE- non-COBRE, 2 external contracts, 2 external COBRE), trained over 60 laboratory personnel, developed 11 new stains and optimized 60 antibodies. In Phase II, the Histology and Imaging Core will maintain existing capabilities and its current outstanding level of performance and expand those capabilities significantly while beginning to prepare for post-award sustainability. Proposed expansion includes the addition of antibody validation services and the acquisition of a two-photon microscope configured for in vivo imaging. The superior abilities of the two-photon system to penetrate tissues and detect faint signals with minimum noise and phototoxicity will enable COBRE investigators to develop innovation in their experimental approaches. These capabilities will have a regional impact, as such an instrument does not currently exist anywhere in southern Maine. Finally, the H&I Core will provide an ambitious program of professional development for Core and COBRE personnel. Critical training opportunities have been identified, including a summer program offered by External Advisory Committee Member Dr. Yves De Koninck’s Neurophotonics Centre at Laval University in nearby Quebec City, Canada. In order for the Core services to be sustained into the future, a fee-for-service plan will be implemented, and an extensive outreach effort will be conducted to broaden the user base. Special care has been taken to ensure that the Core capabilities do not duplicate those offered by other COBRE and INBRE supported research cores. Instead, the Core has strategically specialized in unique services such as a specialty in the processing of non-standard samples in a variety of research models. By these efforts, the UNE H&I Core will provide critical research support to its many users and ensure maximum utility to the broader research community in the State of Maine and neighboring IDeA states.