Histology and Imaging Core

组织学和成像核心

• 批准号: 10176518
• 负责人: DEREK C MOLLIVER
• 金额: $ 22.24万
• 依托单位: UNIVERSITY OF NEW ENGLAND
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-15 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10176518
• 关键词: Address; Advisory Committees; Antibodies; Applications Grants; Award; Biological; Calcium; Canada; Caring; Cell physiology; Cellular Morphology; Centers of Research Excellence; Cities; Committee Members; Communication; Communities; Confocal Microscopy; Contract Services; Contracts; Data; Development; Educational workshop; Ensure; Equipment; Evaluation; Fee-for-Service Plans; Feedback; Fees; Freezing; Funding; Future; Goals; Grant; Histologic; Histology; Human Resources; Image; Image Analysis; Immunohistochemistry; Institution; Laboratories; Laboratory Personnel; Maine; Maintenance; Methods; Microscope; Microscopy; Microtome - medical device; Modeling; New England; Noise; Optics; Pain; Paraffin Embedding; Performance; Phase; Phototoxicity; Pilot Projects; Procedures; Protocols documentation; Quebec; Reporting; Reproducibility; Research; Research Personnel; Research Support; Resources; Sampling; Sensory; Services; Signal Transduction; Stains; Stream; Structure; Surveys; Syncope; System; Technical Expertise; Techniques; Time; Tissue Embedding; Tissues; Training; United States National Institutes of Health; Universities; Validation; Work; base; cohort; cost; cryostat; in vivo imaging; industry partner; innovation; instrument; instrumentation; medical specialties; outreach; programs; research facility; satisfaction; success; summer program; tissue processing; training opportunity; two photon microscopy; two-photon

Phase 1 COBRE support resulted in the successful initiation of the Histology and Imaging Core and its development into a critical resource for COBRE investigators and the research community at the University of New England. The Core’s histology instruments include paraffin embedding and section processing equipment, as well as microtomes, vibratomes and cryostats. Imaging equipment includes bright-field, epifluorescent, stereologic and confocal microscopes. Technical services include immunohistochemistry and antibody optimization. In Phase I, the H&I Core provided services to 18 investigators (8 UNE-COBRE, 6 UNE- non-COBRE, 2 external contracts, 2 external COBRE), trained over 60 laboratory personnel, developed 11 new stains and optimized 60 antibodies. In Phase II, the Histology and Imaging Core will maintain existing capabilities and its current outstanding level of performance and expand those capabilities significantly while beginning to prepare for post-award sustainability. Proposed expansion includes the addition of antibody validation services and the acquisition of a two-photon microscope configured for in vivo imaging. The superior abilities of the two-photon system to penetrate tissues and detect faint signals with minimum noise and phototoxicity will enable COBRE investigators to develop innovation in their experimental approaches. These capabilities will have a regional impact, as such an instrument does not currently exist anywhere in southern Maine. Finally, the H&I Core will provide an ambitious program of professional development for Core and COBRE personnel. Critical training opportunities have been identified, including a summer program offered by External Advisory Committee Member Dr. Yves De Koninck’s Neurophotonics Centre at Laval University in nearby Quebec City, Canada. In order for the Core services to be sustained into the future, a fee-for-service plan will be implemented, and an extensive outreach effort will be conducted to broaden the user base. Special care has been taken to ensure that the Core capabilities do not duplicate those offered by other COBRE and INBRE supported research cores. Instead, the Core has strategically specialized in unique services such as a specialty in the processing of non-standard samples in a variety of research models. By these efforts, the UNE H&I Core will provide critical research support to its many users and ensure maximum utility to the broader research community in the State of Maine and neighboring IDeA states.

第一阶段COBRE的支持导致组织学和成像核心的成功启动， 发展成为COBRE调查人员和研究社区的关键资源， 新英格兰。核心的组织学工具包括石蜡包埋和切片处理 设备，以及切片机，振动器和低温恒温器。成像设备包括明场， 落射荧光显微镜、体视显微镜和共焦显微镜。技术服务包括免疫组织化学和 抗体优化在第一阶段，H&I核心向18名调查员提供了服务（UNE COBRE 8名，UNE COBRE 6名，UNE COBRE 6名）。 非COBRE，2个外部合同，2个外部COBRE），培训了60多名实验室人员，开发了11个 新的染色剂和优化的60种抗体。在第二阶段，组织学和成像核心将保持现有的 能力及其当前出色的性能水平，并显著扩展这些能力， 开始为获奖后的可持续性做准备。拟议的扩展包括增加抗体 验证服务和获取配置用于体内成像的双光子显微镜。上级 双光子系统穿透组织并以最小噪声检测微弱信号的能力， 光毒性将使COBRE研究人员能够在他们的实验方法中进行创新。这些 能力将产生区域影响，因为目前在南部非洲任何地方都没有这样的工具， 缅因州。最后，H&I核心将为核心和 COBRE人员。已经确定了关键的培训机会，包括由 外部咨询委员会成员Yves De Koninck博士在拉瓦尔大学的神经光子学中心， 加拿大魁北克市附近。为了使核心服务在未来持续下去， 计划将得到执行，并将开展广泛的外联工作，以扩大用户基础。 特别注意确保核心能力不与其他核心能力重复。 COBRE和INBRE支持研究核心。相反，核心在战略上专门从事独特的 服务范围如专业从事各种非标样品的加工研究模型。通过 通过这些努力，UNE H&I Core将为其众多用户提供关键的研究支持，并确保最大限度地 在缅因州和邻近的IDEA州的更广泛的研究社区的实用程序。