Histology and Imaging Core

组织学和成像核心

• 批准号: 10176518
• 负责人: DEREK C MOLLIVER
• 金额: $ 22.24万
• 依托单位: UNIVERSITY OF NEW ENGLAND
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-15 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10176518
• 关键词: Address; Advisory Committees; Antibodies; Applications Grants; Award; Biological; Calcium; Canada; Caring; Cell physiology; Cellular Morphology; Centers of Research Excellence; Cities; Committee Members; Communication; Communities; Confocal Microscopy; Contract Services; Contracts; Data; Development; Educational workshop; Ensure; Equipment; Evaluation; Fee-for-Service Plans; Feedback; Fees; Freezing; Funding; Future; Goals; Grant; Histologic; Histology; Human Resources; Image; Image Analysis; Immunohistochemistry; Institution; Laboratories; Laboratory Personnel; Maine; Maintenance; Methods; Microscope; Microscopy; Microtome - medical device; Modeling; New England; Noise; Optics; Pain; Paraffin Embedding; Performance; Phase; Phototoxicity; Pilot Projects; Procedures; Protocols documentation; Quebec; Reporting; Reproducibility; Research; Research Personnel; Research Support; Resources; Sampling; Sensory; Services; Signal Transduction; Stains; Stream; Structure; Surveys; Syncope; System; Technical Expertise; Techniques; Time; Tissue Embedding; Tissues; Training; United States National Institutes of Health; Universities; Validation; Work; base; cohort; cost; cryostat; in vivo imaging; industry partner; innovation; instrument; instrumentation; medical specialties; outreach; programs; research facility; satisfaction; success; summer program; tissue processing; training opportunity; two photon microscopy; two-photon

Phase 1 COBRE support resulted in the successful initiation of the Histology and Imaging Core and its development into a critical resource for COBRE investigators and the research community at the University of New England. The Core’s histology instruments include paraffin embedding and section processing equipment, as well as microtomes, vibratomes and cryostats. Imaging equipment includes bright-field, epifluorescent, stereologic and confocal microscopes. Technical services include immunohistochemistry and antibody optimization. In Phase I, the H&I Core provided services to 18 investigators (8 UNE-COBRE, 6 UNE- non-COBRE, 2 external contracts, 2 external COBRE), trained over 60 laboratory personnel, developed 11 new stains and optimized 60 antibodies. In Phase II, the Histology and Imaging Core will maintain existing capabilities and its current outstanding level of performance and expand those capabilities significantly while beginning to prepare for post-award sustainability. Proposed expansion includes the addition of antibody validation services and the acquisition of a two-photon microscope configured for in vivo imaging. The superior abilities of the two-photon system to penetrate tissues and detect faint signals with minimum noise and phototoxicity will enable COBRE investigators to develop innovation in their experimental approaches. These capabilities will have a regional impact, as such an instrument does not currently exist anywhere in southern Maine. Finally, the H&I Core will provide an ambitious program of professional development for Core and COBRE personnel. Critical training opportunities have been identified, including a summer program offered by External Advisory Committee Member Dr. Yves De Koninck’s Neurophotonics Centre at Laval University in nearby Quebec City, Canada. In order for the Core services to be sustained into the future, a fee-for-service plan will be implemented, and an extensive outreach effort will be conducted to broaden the user base. Special care has been taken to ensure that the Core capabilities do not duplicate those offered by other COBRE and INBRE supported research cores. Instead, the Core has strategically specialized in unique services such as a specialty in the processing of non-standard samples in a variety of research models. By these efforts, the UNE H&I Core will provide critical research support to its many users and ensure maximum utility to the broader research community in the State of Maine and neighboring IDeA states.

第一阶段 COBRE 支持成功启动了组织学和成像核心及其 发展成为 COBRE 研究人员和大学研究界的重要资源 新英格兰。 Core 的组织学仪器包括石蜡包埋和切片处理 设备，以及切片机、振动切片机和低温恒温器。成像设备包括明场、 落射荧光显微镜、体视显微镜和共焦显微镜。技术服务包括免疫组化和 抗体优化。在第一阶段，H&I 核心为 18 名研究人员提供了服务（8 位 UNE-COBRE、6 位 UNE- 非 COBRE，2 个外部合同，2 个外部 COBRE），培训了 60 多名实验室人员，开发了 11 新染色剂和优化的 60 种抗体。在第二阶段，组织学和成像核心将维持现有的 能力及其当前出色的性能水平，并显着扩展这些能力，同时 开始为获奖后的可持续性做准备。拟议的扩展包括添加抗体 验证服务并购买配置用于体内成像的双光子显微镜。上级 双光子系统穿透组织并以最小的噪声检测微弱信号的能力 光毒性将使 COBRE 研究人员能够在实验方法上进行创新。这些 能力将产生区域影响，因为目前南方任何地方都不存在这样的文书 缅因州。最后，H&I 核心将为核心和 I 核心提供雄心勃勃的专业发展计划。 COBRE 人员。已经确定了关键的培训机会，包括由 外部顾问委员会成员 Yves De Koninck 博士位于拉瓦尔大学的神经光子学中心 加拿大魁北克市附近。为了使核心服务能够持续到未来，按服务收费 计划将得到实施，并将进行广泛的外展工作以扩大用户基础。 我们特别注意确保核心功能不会与其他产品提供的功能重复。 COBRE 和 INBRE 支持研究核心。相反，核心战略性地专注于独特的领域 专业从事各种研究模型的非标样品加工等服务。经过 通过这些努力，UNE H&I 核心将为其众多用户提供关键的研究支持，并确保最大限度地 对缅因州和邻近的 IDeA 州更广泛的研究界具有实用性。