Shared Resource: Cellular Imaging

项目概要：细胞成像共享资源（CISR） 细胞成像共享资源（CISR）提供广泛的高级成像支持， 用于探测细胞和模式生物的结构和功能的定量图像分析技术 从分子水平到细胞水平。支持的应用包括扫描和透射电子 显微镜、电子显微镜（EM）样品制备、透射光和荧光显微镜 模态（包括共聚焦、去卷积、全内反射荧光显微镜（TIRF）、超显微镜（ultrasound））、 分辨率、延时、全载玻片扫描和高内容筛选），以及基于实时图像的细胞 最近实施的检测，以支持不断增长的癌症免疫学研究计划。额外 服务包括定量凝胶和印迹分析以及核型分析。CISR由陈博士领导。 Julio Vazquez由两名工作人员（1.65 FTE）和一名外部顾问协助。 在2015-2018年期间，CISR为大约150个用户中的500多个个人用户提供了服务。 代表所有联盟研究项目的研究小组。仅在2018财年，CISR就支持了 200多项研究赠款。CISR为联盟引入了许多新技术，支持和 通过提供领先的仪器和专业知识，培训新用户， 每一种技术，并与研究人员在所有支持的技术密切合作。虽然许多实验室 他们自己的仪器进行日常工作，许多先进的技术只有在CISR。 1

PROJECT SUMMARY: CELLULAR IMAGING SHARED RESOURCE (CISR) The Cellular Imaging Shared Resource (CISR) provides support with a broad range of advanced imaging and quantitative image analysis technologies for probing the structure and function of cells and model organisms from the molecular to the cellular level. Supported applications include scanning and transmission electron microscopy, electron microscopy (EM) sample preparation, transmitted light and fluorescence microscopy modalities (including confocal, deconvolution, total internal reflection fluorescence microscopy (TIRF), super- resolution, time-lapse, whole slide scanning, and high-content screening), and live image-based cellular assays recently implemented to support the growing Cancer Immunology Research Program. Additional services include quantitative gel and blot analysis as well as karyotyping. CISR is under the leadership of Dr. Julio Vazquez who is assisted by two staff members (1.65 FTE) and one external consultant. For the period 2015-2018, CISR provided services to more than 500 individual users from approximately 150 research groups representing all of the Consortium’s Research Programs. In FY 2018 alone, CISR supported over 200 research grants. CISR has introduced many new technologies to the Consortium, supports and accelerates cancer research by providing leading edge instrumentation and expertise, training users new to each technique, and working closely with researchers in all supported technologies. While many labs have their own instruments for routine work, many advanced technologies are only available in the CISR. 1