Shared Resource: Cellular Imaging

细胞成像共享资源 项目总结/摘要 细胞成像共享资源（CISR）为联盟成员提供了一套全面的 先进的高分辨率成像技术，定量凝胶和印迹成像，以及定量图像 癌症研究的分析。该资源由两个基于服务的实验室组成，Electron 显微镜（EM）和科学成像（SI）。EM实验室提供样品制备、 切片术，冷冻和免疫EM技术，并在传输和扫描EM培训，原子 力显微镜SI实验室提供先进的成像系统， 反卷积，多光子，时间推移，全内反射荧光，高含量分析，整片 扫描和其他光学显微镜技术，沿着强大的可视化和定量软件， 显微镜数据分析。随着研究需求和优先事项的发展，CI已扩展到新的领域， 为更广泛的调查员和研究计划提供支持。新服务的例子包括： 高含量分析和平板筛选;全载玻片扫描和分析;以及冷冻和免疫-EM。 总的来说，所有联盟的研究计划目前使用的资源和两个组成部分提供了必要的 为会员提供研究服务和技术。该资源不断评估新技术和 他们可以给联盟带来的好处，并通过课程积极促进这些技术的使用， 讲习班、研讨会和与个别研究人员的一对一讨论。

Cellular Imaging Shared Resource Project Summary/Abstract The Cellular Imaging Shared Resource (CISR) provides Consortium members with a comprehensive set of advanced high resolution imaging techniques, quantitative gel and blot imaging, and quantitative image analysis for cancer research. The resource is comprised of two service-based laboratories, Electron Microscopy (EM) and Scientific Imaging (SI). The EM laboratory offers sample preparation, access to microtomy, cryo- and immuno- EM technologies, and training in transmission and scanning EM, and atomic force microscopy. The SI laboratory provides access to advanced imaging systems for confocal, deconvolution, multi photon, time lapse, total internal reflection fluorescence, high content analysis, whole slide scanning, and other light microscopy techniques, along with powerful software for visualization and quantitative analysis of microscopy data. As research needs and priorities evolve, CI has expanded into new areas to provide support for a broader range of investigator and research programs. Examples of new services include: high content analysis and plate screening; whole slide scanning and analysis; and Cryo and Immuno-EM. Overall, all Consortium research programs currently use the resource and both components provide essential services and technologies for member research. The resource constantly evaluates new technologies and the benefits they can bring to the Consortium, and actively promotes the use of such technologies through classes, workshops, seminars, and one-on-one discussions with individual researchers.