Mentoring Patient Oriented Research in sensory disorders

指导以患者为导向的感觉障碍研究

• 批准号: 10644567
• 负责人: Alan Gi-Lun Cheng
• 金额: $ 19.67万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10644567
• 关键词: Acceleration; Acoustic Neuroma; Address; Adult; Animal Model; Auditory; Autopsy; Award; Bioinformatics; Biological Models; Capsid; Cell Maturation; Cells; Chickens; Cochlea; Cryoultramicrotomy; Cytomegalovirus; Dependovirus; Development; Disease; Dissociation; Dizziness; Down-Regulation; Equilibrium; Excision; Fetal Tissues; Functional disorder; Genes; Genetic; Glial Fibrillary Acidic Protein; Goals; Hair Cells; Histologic; Human; Immunohistochemistry; In Situ; In Situ Hybridization; In Vitro; Institution; Investigation; Knowledge; Labyrinth; Mechanoreceptor Cell; Medical; Medical Records; Mentors; Mentorship; Methods; Mid-Career Clinical Scientist Award (K24); Molecular; Molecular Profiling; Mus; Natural regeneration; Operative Surgical Procedures; Organ; Organ Donor; Patients; Recording of previous events; Research; Research Personnel; Sensory; Sensory Disorders; Sensory Hair; Supporting Cell; Technology; Temporal bone structure; Testing; Therapeutic; Time; Tissues; Training; Translations; Tropism; Utricle structure; Vertigo; Vestibular Hair Cells; Zebrafish; career; cohort; design; differential expression; effective therapy; experience; experimental study; gain of function; gene therapy; hair cell regeneration; hair regeneration; human disease; in vivo; novel; overexpression; patient oriented research; promoter; recruit; restoration; single-cell RNA sequencing; skills; stem cells; tool; transcription factor; transcriptome; transduction efficiency

Abstract: Sensory hair cells are required for balance function. Vestibular hair cell degeneration causes balance dysfunction manifested as dizziness and vertigo. In mice, a limited degree of spontaneous regeneration occurs in the utricle, a vestibular organ detecting linear acceleration. Moreover, addition of the transcription factor Atoh1 robust enhances regeneration of hair cells in the mouse utricle, but regenerated hair cells mature only partially relative to native hair cells. In preliminary experiments, we have characterized a novel AAV-ATOH1 construct leading to regeneration of more mature hair cells in the mouse utricle. With the long-term goal of regenerating human hair cells to restore balance function, two major obstacles remain: 1) we have an incomplete understanding of the molecular signatures of human hair cells, supporting cells and hair cell precursors, 2) adult human inner ear tissues are not readily available to test promising therapeutics discovered in animal models. To overcome these obstacles, we have designed a surgical method to procure live utricles from deceased organ donors who typically have normal auditory and vestibular function. We have begun assembling their medical records, single-cell transcriptomes, and histologic sections of utricles. In parallel, we have procured utricles from vestibular schwannoma patients undergoing surgical resection. Here, we propose to increase the recruitment of organ donors and vestibular schwannoma patients and delineate and validate the transcriptomes of hair cells, supporting cells, and hair cell precursors in adult human utricles in these two cohorts (Aim 1). Furthermore, we will determine whether our novel AAV-ATOH1 enhances hair regeneration and maturation in cultured human utricles (Aim 2). Another goal of this award is to further my career as an investigator and mentor in patient- oriented research. To build on my previous experience in bench research and mentoring, this award is designed to protect my time and help me gain knowledge and skills to 1) study human hair cell regeneration and 2) mentor others pursuing patient-oriented research. In summary, we will apply state-of-the art technologies (single-cell RNA-sequencing, gene therapy, bioinformatic strategies) to study vestibular hair cell regeneration in live human utricles. We have assembled a team of collaborators and experienced mentors in patient-oriented research. At the end of this 5-year proposal, we will have 1) delineated transcriptomes of human hair cells, supporting cells, and hair cell precursors, 2) revealed whether novel AAV-ATOH1 enhances regeneration and maturation of human vestibular hair cells, and 3) enhanced my ability to perform and mentor others in patient-oriented research in sensory disorders.

摘要： 感觉毛细胞需要平衡功能。前庭毛细胞退化导致平衡 功能障碍表现为头晕和眩晕。在小鼠中，有限程度的自发再生 发生在椭圆囊中，椭圆囊是检测线性加速度的前庭器官。此外，除了 转录因子Atoh 1增强了小鼠椭圆囊毛细胞的再生，但 再生毛细胞相对于天然毛细胞仅部分成熟。在初步实验中，我们 已经表征了一种新型AAV-ATOH 1构建体，该构建体导致更成熟的毛细胞再生， 老鼠的椭圆囊随着人类毛细胞再生以恢复平衡功能的长期目标， 两个主要障碍仍然存在：1）我们对分子特征的理解不完全 人毛细胞、支持细胞和毛细胞前体，2）成人内耳组织 并不容易用于测试在动物模型中发现的有希望的治疗方法。 为了克服这些障碍，我们设计了一种手术方法， 通常具有正常听觉和前庭功能的已故器官捐献者。我们已经开始 收集他们的医疗记录、单细胞转录组和椭圆囊的组织切片。在 同时，我们也从接受手术切除的前庭神经鞘瘤患者中获得椭圆囊。 在此，我们建议增加器官捐献者和前庭神经鞘瘤患者的招募 并描绘和验证毛细胞，支持细胞和毛细胞前体的转录组， 这两个组群中的成人椭圆囊（Aim 1）。此外，我们将确定我们的小说是否 AAV-ATOH 1增强培养的人椭圆囊中的毛发再生和成熟（目的2）。 这个奖项的另一个目标是进一步我的职业生涯作为一个调查员和导师在病人- 导向研究。为了建立在我以前的经验，在板凳研究和指导，这个奖项 是为了保护我的时间，帮助我获得知识和技能，1）研究人类毛细胞 再生和2）指导其他人追求以病人为导向的研究。 总之，我们将应用最先进的技术（单细胞RNA测序，基因测序，基因测序）， 治疗，生物信息学策略）来研究活体人类椭圆囊中的前庭毛细胞再生。我们 已经组建了一个合作者和经验丰富的导师团队，以患者为导向的研究。在 在这个为期5年的提案结束时，我们将有1）划定的人类毛细胞转录组，支持 细胞和毛细胞前体，2）揭示了新的AAV-ATOH 1是否能增强再生， 人类前庭毛细胞的成熟，和3）增强了我的能力，执行和指导他人， 以病人为中心的感觉障碍研究。