Mentoring Patient Oriented Research in sensory disorders
指导以患者为导向的感觉障碍研究
基本信息
- 批准号:10644567
- 负责人:
- 金额:$ 19.67万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-05-01 至 2028-04-30
- 项目状态:未结题
- 来源:
- 关键词:AccelerationAcoustic NeuromaAddressAdultAnimal ModelAuditoryAutopsyAwardBioinformaticsBiological ModelsCapsidCell MaturationCellsChickensCochleaCryoultramicrotomyCytomegalovirusDependovirusDevelopmentDiseaseDissociationDizzinessDown-RegulationEquilibriumExcisionFetal TissuesFunctional disorderGenesGeneticGlial Fibrillary Acidic ProteinGoalsHair CellsHistologicHumanImmunohistochemistryIn SituIn Situ HybridizationIn VitroInstitutionInvestigationKnowledgeLabyrinthMechanoreceptor CellMedicalMedical RecordsMentorsMentorshipMethodsMid-Career Clinical Scientist Award (K24)MolecularMolecular ProfilingMusNatural regenerationOperative Surgical ProceduresOrganOrgan DonorPatientsRecording of previous eventsResearchResearch PersonnelSensorySensory DisordersSensory HairSupporting CellTechnologyTemporal bone structureTestingTherapeuticTimeTissuesTrainingTranslationsTropismUtricle structureVertigoVestibular Hair CellsZebrafishcareercohortdesigndifferential expressioneffective therapyexperienceexperimental studygain of functiongene therapyhair cell regenerationhair regenerationhuman diseasein vivonoveloverexpressionpatient oriented researchpromoterrecruitrestorationsingle-cell RNA sequencingskillsstem cellstooltranscription factortranscriptometransduction efficiency
项目摘要
Abstract:
Sensory hair cells are required for balance function. Vestibular hair cell degeneration causes balance
dysfunction manifested as dizziness and vertigo. In mice, a limited degree of spontaneous regeneration
occurs in the utricle, a vestibular organ detecting linear acceleration. Moreover, addition of the
transcription factor Atoh1 robust enhances regeneration of hair cells in the mouse utricle, but
regenerated hair cells mature only partially relative to native hair cells. In preliminary experiments, we
have characterized a novel AAV-ATOH1 construct leading to regeneration of more mature hair cells in
the mouse utricle. With the long-term goal of regenerating human hair cells to restore balance function,
two major obstacles remain: 1) we have an incomplete understanding of the molecular signatures
of human hair cells, supporting cells and hair cell precursors, 2) adult human inner ear tissues
are not readily available to test promising therapeutics discovered in animal models.
To overcome these obstacles, we have designed a surgical method to procure live utricles from
deceased organ donors who typically have normal auditory and vestibular function. We have begun
assembling their medical records, single-cell transcriptomes, and histologic sections of utricles. In
parallel, we have procured utricles from vestibular schwannoma patients undergoing surgical resection.
Here, we propose to increase the recruitment of organ donors and vestibular schwannoma patients
and delineate and validate the transcriptomes of hair cells, supporting cells, and hair cell precursors in
adult human utricles in these two cohorts (Aim 1). Furthermore, we will determine whether our novel
AAV-ATOH1 enhances hair regeneration and maturation in cultured human utricles (Aim 2).
Another goal of this award is to further my career as an investigator and mentor in patient-
oriented research. To build on my previous experience in bench research and mentoring, this award
is designed to protect my time and help me gain knowledge and skills to 1) study human hair cell
regeneration and 2) mentor others pursuing patient-oriented research.
In summary, we will apply state-of-the art technologies (single-cell RNA-sequencing, gene
therapy, bioinformatic strategies) to study vestibular hair cell regeneration in live human utricles. We
have assembled a team of collaborators and experienced mentors in patient-oriented research. At the
end of this 5-year proposal, we will have 1) delineated transcriptomes of human hair cells, supporting
cells, and hair cell precursors, 2) revealed whether novel AAV-ATOH1 enhances regeneration and
maturation of human vestibular hair cells, and 3) enhanced my ability to perform and mentor others in
patient-oriented research in sensory disorders.
摘要:
感觉毛细胞需要平衡功能。前庭毛细胞退化导致平衡
功能障碍表现为头晕和眩晕。在小鼠中,有限程度的自发再生
发生在椭圆囊中,椭圆囊是检测线性加速度的前庭器官。此外,除了
转录因子Atoh 1增强了小鼠椭圆囊毛细胞的再生,但
再生毛细胞相对于天然毛细胞仅部分成熟。在初步实验中,我们
已经表征了一种新的AAV-ATOH 1构建体,其导致更成熟的毛细胞再生,
老鼠的椭圆囊随着人类毛细胞再生以恢复平衡功能的长期目标,
两个主要障碍仍然存在:1)我们对分子特征的理解不完全
人毛细胞、支持细胞和毛细胞前体,2)成人内耳组织
并不容易用于测试在动物模型中发现的有希望的治疗方法。
为了克服这些障碍,我们设计了一种手术方法,
通常具有正常听觉和前庭功能的已故器官捐献者。我们已经开始
收集他们的医疗记录、单细胞转录组和椭圆囊的组织切片。在
同时,我们也从接受手术切除的前庭神经鞘瘤患者中获得椭圆囊。
在此,我们建议增加器官捐献者和前庭神经鞘瘤患者的招募
并描绘和验证毛细胞,支持细胞和毛细胞前体的转录组,
这两个组群中的成人椭圆囊(Aim 1)。此外,我们将确定我们的小说是否
AAV-ATOH 1增强培养的人椭圆囊中的毛发再生和成熟(目的2)。
这个奖项的另一个目标是进一步我的职业生涯作为一个调查员和导师在病人-
导向研究。为了建立在我以前的经验,在板凳研究和指导,这个奖项
是为了保护我的时间,帮助我获得知识和技能,1)研究人类毛细胞
再生和2)指导其他人追求以病人为导向的研究。
总之,我们将应用最先进的技术(单细胞RNA测序,基因测序,基因测序),
治疗,生物信息学策略)来研究活体人类椭圆囊中的前庭毛细胞再生。我们
已经组建了一个合作者和经验丰富的导师团队,以患者为导向的研究。在
在这个为期5年的提案结束时,我们将有1)划定的人类毛细胞转录组,支持
细胞和毛细胞前体,2)揭示了新的AAV-ATOH 1是否能增强再生,
人类前庭毛细胞的成熟,和3)增强了我的能力,执行和指导他人,
以病人为中心的感觉障碍研究。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Alan Gi-Lun Cheng其他文献
Alan Gi-Lun Cheng的其他文献
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{{ truncateString('Alan Gi-Lun Cheng', 18)}}的其他基金
Diversification of the mechanotransduction complex in vestibular hair cells
前庭毛细胞中机械转导复合体的多样化
- 批准号:
10734358 - 财政年份:2023
- 资助金额:
$ 19.67万 - 项目类别:
Molecular basis of mammalian cochlear regeneration
哺乳动物耳蜗再生的分子基础
- 批准号:
10682272 - 财政年份:2023
- 资助金额:
$ 19.67万 - 项目类别:
Stanford Clinician Scientist Training Program
斯坦福临床医生科学家培训计划
- 批准号:
10427050 - 财政年份:2022
- 资助金额:
$ 19.67万 - 项目类别:
Stanford Clinician Scientist Training Program
斯坦福临床医生科学家培训计划
- 批准号:
10591580 - 财政年份:2022
- 资助金额:
$ 19.67万 - 项目类别:
Clinician-scientist training program in otolaryngology
耳鼻喉科临床医生科学家培训计划
- 批准号:
10368168 - 财政年份:2016
- 资助金额:
$ 19.67万 - 项目类别:
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