Genetic Basis of Dorso-Ventral Patterning in the Drosophila Eye

果蝇眼背腹模式的遗传基础

基本信息

  • 批准号:
    10652488
  • 负责人:
  • 金额:
    $ 32.85万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-08-01 至 2026-06-30
  • 项目状态:
    未结题

项目摘要

In multi-cellular organisms, axial patterning is required for transition of a mono-layered epithelium of an organ primordium to a three-dimensional organ by delineation of antero-posterior (AP), dorso-ventral (DV), and proximo-distal (PD) axis. We use Drosophila melanogaster (fruit fly) eye model to study the highly conserved fundamental process of (axial) DV patterning and growth. During eye development, DV patterning precedes AP and PD axis patterning, and forms dorsal and ventral compartments. Discerning the mechanism of axes determination (DV) is crucial for our understanding of organogenesis as the problems with DV delineation results in developmental/ birth defects in flies to humans. Our long term goal is to understand the genetic basis of DV patterning which is established by interactions of the dorsal selector genes and the ventral genes. The Drosophila eye begins from a ventral equivalent state on which the dorsal fate is established by onset of expression of GATA-family transcription factor Pannier (Pnr), the secreted morphogen Wingless (Wg), and Iroquois (Iro-C) family proteins. In the dorsal eye, pnr is not the sole regulator of Wg expression. It strongly suggests that there may be other dorsal eye genes that are yet to be identified. We have identified a new dorsal eye selector defective proventriculus (dve), a transcription factor, which acts upstream of wg. To understand the molecular genetic basis of DV patterning, we will analyze the (1) Investigate wg regulation in the dorsal eye. (2) How Wg gradient from dorsal eye is interpreted for eye versus head fate? (3) Test if Hippo signaling co- regulate Wg signaling with DV patterning genes in the eye. Given that the genetic machinery is conserved, we will also test the role of SATB1, a human ortholog of dve in the eye. Our study will have significant bearing on (i) developmental mechanisms of lineage restriction and patterning that follow DV patterning during organogenesis, (ii) role of growth regulatory gene in patterning and (iii) the understanding of etiology of early childhood retinal diseases. (iv) How do independent pathways interact to regulate growth and patterning in the developing eye? The proposed studies will generate insights into (1) how dorsal eye genes interact to determine eye versus head fate by regulating Wg signaling, (2) How two independent pathways like Hippo signaling and DV patterning coregulate Wg signaling to promote growth and patterning in the developing eye? (3) SATB1 is mainly known to regulate growth and our studies in eye will provide insight into growth regulation and patterning function of SATB1. These studies will have significant bearings on understanding the genetic mechanism of early developmental events during organogenesis in higher vertebrates. The knowledge generated from these studies is expected to elucidate fundamental mechanisms in patterning and growth of normal visual function and within the context of retinal disease and birth defects in the eye.
在多细胞生物体中,轴向图案化是细胞的单层上皮转变所必需的。 通过描绘前后(AP)、背腹(dorso-ventral) (DV)和近端-远端(PD)轴。我们使用果蝇(Drosophila melanogaster)眼模型来研究 (轴向)DV图案化和生长的高度保守的基本过程。在眼睛发育过程中,DV 图案化先于AP和PD轴图案化,并形成背侧和腹侧隔室。挑剔 轴决定(DV)的机制对于我们理解器官发生是至关重要的, DV描绘的问题导致从果蝇到人类的发育/出生缺陷。我们的长期 目的是了解DV模式的遗传基础,DV模式是通过背侧肌和背侧肌的相互作用建立的。 选择基因和腹侧基因。果蝇的眼睛从腹侧的等效状态开始, 其背侧命运通过GATA家族转录因子Pannier的表达开始建立, (Pnr)分泌型形态发生蛋白Wingless(Wg)和Iroquois(Iro-C)家族蛋白。在背侧的眼睛里, 不是Wg表达的唯一调节因子。这强烈表明可能还有其他的背眼基因 尚未被确认我们发现了一种新的背侧眼选择器缺陷前胃(dve), 转录因子,其作用于WG的上游。了解DV的分子遗传学基础 图案化,我们将分析(1)研究背眼中的wg调节。(2)如何Wg 从背侧眼的梯度被解释为眼与头的命运?(3)测试河马信号是否合- 用眼睛中的DV图案化基因调节Wg信号传导。鉴于遗传机制是 保守,我们也将测试SATB 1的作用,SATB 1是眼睛中dve的人类直系同源物。我们的研究将有 与(i)DV后谱系限制和模式化的发育机制有重要关系 在器官发生过程中的模式化,(ii)生长调节基因在模式化中的作用,以及(iii) 了解儿童早期视网膜疾病的病因。(iv)独立的通路如何相互作用 来调节发育中的眼睛的生长和图案?拟议的研究将产生对以下方面的见解: (1)背眼基因如何通过调节Wg信号相互作用来决定眼睛与头部的命运,(2)如何 两个独立的途径,如Hippo信号传导和DV模式共同调节Wg信号传导,以促进 在发育中的眼睛中生长和形成图案?(3)已知SATB 1主要调节生长, 对眼睛的研究将为深入了解SATB 1的生长调节和图案功能提供帮助。这些研究 将对理解早期发育事件的遗传机制具有重要意义 在高等脊椎动物的器官发生过程中。从这些研究中产生的知识预计将 阐明正常视觉功能的模式和生长的基本机制, 视网膜疾病和先天缺陷的背景下在眼睛。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Madhuri Kango-Singh其他文献

Madhuri Kango-Singh的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Madhuri Kango-Singh', 18)}}的其他基金

Cell-Cell Interactions In Alzheimer's disease and related dementias
阿尔茨海默病和相关痴呆症中的细胞间相互作用
  • 批准号:
    10711899
  • 财政年份:
    2021
  • 资助金额:
    $ 32.85万
  • 项目类别:
Genetic Basis of Dorso-Ventral Patterning in the Drosophila Eye
果蝇眼背腹模式的遗传基础
  • 批准号:
    10279832
  • 财政年份:
    2021
  • 资助金额:
    $ 32.85万
  • 项目类别:
Genetic Basis of Dorso-Ventral Patterning in the Drosophila Eye
果蝇眼背腹模式的遗传基础
  • 批准号:
    10459551
  • 财政年份:
    2021
  • 资助金额:
    $ 32.85万
  • 项目类别:

相似海外基金

How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
  • 批准号:
    BB/Z514391/1
  • 财政年份:
    2024
  • 资助金额:
    $ 32.85万
  • 项目类别:
    Training Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
  • 批准号:
    2312555
  • 财政年份:
    2024
  • 资助金额:
    $ 32.85万
  • 项目类别:
    Standard Grant
RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
  • 批准号:
    2327346
  • 财政年份:
    2024
  • 资助金额:
    $ 32.85万
  • 项目类别:
    Standard Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
  • 批准号:
    ES/Z502595/1
  • 财政年份:
    2024
  • 资助金额:
    $ 32.85万
  • 项目类别:
    Fellowship
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
  • 批准号:
    ES/Z000149/1
  • 财政年份:
    2024
  • 资助金额:
    $ 32.85万
  • 项目类别:
    Research Grant
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
  • 批准号:
    23K24936
  • 财政年份:
    2024
  • 资助金额:
    $ 32.85万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
  • 批准号:
    2901648
  • 财政年份:
    2024
  • 资助金额:
    $ 32.85万
  • 项目类别:
    Studentship
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
  • 批准号:
    488039
  • 财政年份:
    2023
  • 资助金额:
    $ 32.85万
  • 项目类别:
    Operating Grants
New Tendencies of French Film Theory: Representation, Body, Affect
法国电影理论新动向:再现、身体、情感
  • 批准号:
    23K00129
  • 财政年份:
    2023
  • 资助金额:
    $ 32.85万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The Protruding Void: Mystical Affect in Samuel Beckett's Prose
突出的虚空:塞缪尔·贝克特散文中的神秘影响
  • 批准号:
    2883985
  • 财政年份:
    2023
  • 资助金额:
    $ 32.85万
  • 项目类别:
    Studentship
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了