Aptamer-functionalized cardiac patches

项目摘要 缺血性心脏病影响约1500万美国人，每天约有2,150人死亡。的能力 开发用于局部递送治疗剂的多功能植入物具有巨大的治疗潜力 心肌梗死（MI），今天仍然具有挑战性。这个项目的目标是开发一个新的 具有促进血管生成和抗炎两种主要功能的心脏补片。引人注目 已经收集了初步结果，显示核酸适体可以掺入水凝胶中 具有螯合和释放治疗剂的能力。基于令人信服的结果，该项目 开发具有三个具体目标，包括1）开发用于治疗心脏病的适体功能化的心脏贴片， 血管生成因子的受控释放，2）评价贴剂在促进血管生成中的功能性， 治疗性血管生成和MI治疗，以及3）治疗性血管生成和抗炎的评价 用于协同MI治疗。预期适体官能化的贴片将稳定地螯合 治疗性蛋白质并以持续的方式释放它们;以及多种信号分子的共同递送 将导致协同MI治疗。该项目的成功具有开辟新途径的巨大潜力 用于治疗缺血性心脏病患者的心脏植入物。

PROJECT SUMMARY Ischemic heart disease affects ~15 million Americans and accounts for ~2,150 deaths every day. The ability to develop multifunctional implants for local delivery of therapeutic agents holds great potential for the treatment of myocardial infarction (MI), which remains challenging today. The objective of this project is to develop a novel cardiac patch with two primary functions of promoting both angiogenesis and anti-inflammation. Compelling preliminary results have been collected, showing that nucleic acid aptamers can be incorporated into hydrogels with the capability of sequestering and releasing therapeutic agents. Based on the compelling results, this project is developed with three specific aims including 1) development of aptamer-functionalized cardiac patches for controlled release of angiogenic factors, 2) evaluation of the functionality of the patches in the promotion of therapeutic angiogenesis and MI treatment, and 3) evaluation of therapeutic angiogenesis and anti-inflammation for synergistic MI treatment. It is anticipated that aptamer-functionalized patches would stably sequester therapeutic proteins and release them in a sustained manner; and that co-delivery of multiple signaling molecules would lead to synergistic MI treatment. The success of this project holds great potential to open a new avenue of developing cardiac implants for the treatment of patients with ischemic heart disease.