Fluorescence-Solid Phase Extraction (F-SPE) Platform for Rapid, On-site Detection of PFAS

用于快速现场检测 PFAS 的荧光固相萃取 (F-SPE) 平台

• 批准号: 10699514
• 负责人: Dhruv Patel
• 金额: $ 27.63万
• 依托单位: ESPIRA, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-16 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10699514
• 关键词: Abate; Acute; Adverse effects; Applications Grants; Area; Biodegradation; Carpet; Certification; Characteristics; Chemicals; Clothing; Collection; Consumption; Coupled; Detection; Detergents; Development; Development Plans; Devices; Disease; Endocrine; Equilibrium; Evaluation; Exposure to; Fertility; Fire - disasters; Fluorescence; Food; Health; High Pressure Liquid Chromatography; Human; Hydrogen Bonding; Hydrolysis; Immobilization; Immune; Individual; Industry; Intellectual Property; Laboratories; Liquid Chromatography; Literature; Marketing; Mass Spectrum Analysis; Membrane; Metabolic; Metabolism; Methods; Military Personnel; Nature; Optics; Phase; Poly-fluoroalkyl substances; Polymers; Porosity; Pregnancy Complications; Preparation; Procedures; Process; Reporting; Reproductive system; Research; Resistance; Resource-limited setting; Risk; Sampling; Screening procedure; Shipping; Site; Small Business Innovation Research Grant; Solid; Source; Specificity; Speed; Sunlight; Surface; Surveys; Systems Integration; Techniques; Technology; Testing; Time; Water; Water Pollutants; air sampling; analytical method; cancer risk; chemical bond; chemical stability; commercialization; consumer product; cost; design; detection limit; drinking; drinking water; fluorophore; innovation; manufacture; meetings; meter; nanomolar; operation; perfluorooctane sulfonate; perfluorooctanoic acid; performance tests; photolysis; portability; screening; sensor; sensor technology; silochrome; skills; software systems; soil sampling; stability testing; substance use; well water

Project Summary Per-and polyfluoroalkyl substances (PFAS) have become an emerging class of water pollutants that cause serious environmental and health concerns. Due to their wide use in industry, military, and fire protection, PFAS have been spread and present in all kinds of water bodies. Among the over thousand PFAS ever manufactured and used, perfluorooctanesulfonic (PFOS) and perfluorooctanoic acid (PFOA) represent the top two PFAS used and studied the most for health effects. Recent studies indicate a tight linkage between exposure to these PFAS and many kinds of diseases and health effects. Currently, the advisory level set by the US EPA set for PFOS and PFOA in drinking water is 70 ppt (0.14 and 0.17 nM for PFOS and PFOA). Current detection of PFAS is mostly based on liquid chromatography coupled with mass spectrometry (LC-MS). However, the costs, footprint, power requirements, and sample preparation processes often associated with the LC-MS technologies limit their deployment beyond the formal laboratory setting. Especially for the detection of nanomolar levels of PFAS, LC-MS usually requires a preconcentration frontend device, making the analysis even more time-consuming. It becomes imperative to develop a rapid, simple, and low-cost sensor technology that is more suited for quick onsite detection of PFAS. While many chemical sensors, such as those based on fluorescence modulation, have been developed for the detection of PFAS, most of them are still far from sufficient for potable water analysis regarding either sensitivity (vs. 70 ppt) or selectivity (against the common chemicals, especially detergents). This project aims to fill this technical gap by developing a unique sensor platform that is small and easy to use, offering sensitive and selective infield detection of PFOS and PFOA (selected as the representative PFAS analytes). The sensor platform is based on highly sensitive and selective fluorescence sensors coated onto solid-phase extraction (SPE) capable of preconcentration of low concentrations of analyses, thus lowering the detection limit. The combination of preconcentration of SPE and fluorescence detection in one platform (namely F-SPE) would significantly simplify and speed up the analysis process. Moreover, F-SPE takes the principle of negligible depletion (ND) intrinsic to SPE, which would further simplify the analysis process by eliminating the need to precisely meter the sample volume as typically required for conventional analytical methods. ND relies on passing the minimal amount of sample through the membrane that is required for the analyte extraction to reach equilibrium. At this point, the analyte concentrations in the sample entering and exiting the membrane are equal. As a result, the surface concentration of the analyte can be directly correlated to its concentration in the sample but is no longer dependent on the volume of the sample passed through the membrane. Therefore, it is no longer necessary to meter an exact sample volume through the membrane. The main innovation herein lies in integrating the high sensitivity and selectivity of fluorescence sensors with the preconcentration capability and ND principle of SPE, which will enable quick, reliable detection of PFAS in a simple, low-cost way. The project will be implemented around three specific aims: Specific Aim 1. Synthesis and surface immobilization of fluorophores selective to either PFOS or PFOA to in-house fabricated SPE disks. Specific Aim 2. Evaluation of F-SPE/ND for PFOS and PFOA detection. Specific Aim 3. Commercialization Assessment.

项目摘要 每氟烷基物质（PFA）已成为新兴的水污染物 这引起严重的环境和健康问题。由于它们在行业中广泛使用，军事用途 以及防火，PFA已在各种水体中散布并存在。在 超过千的PFA制造和使用，PerfluorOoctanesulfonic（PFO）和 全氟辛酸（PFOA）代表了使用的前两个PFA，并研究了最大的健康状况 效果。最近的研究表明，暴露于这些PFA和多种类型之间存在紧密联系 疾病和健康影响。目前，美国EPA设置的咨询级别为PFO和 饮用水中的PFOA为70 ppt（PFO和PFOA的0.14和0.17 nm）。当前检测 PFA主要基于液相色谱和质谱法（LC-MS）。 但是，经常的成本，足迹，电源需求和样本准备过程 与LC-MS技术相关的技术将其部署限制在正式实验室之外 环境。特别是对于检测纳摩尔水平的PFA，LC-MS通常需要 预浓缩前端设备，使分析更加耗时。它变成了 必须开发快速，低成本的传感器技术，更适合快速 PFA的现场检测。而许多化学传感器，例如基于荧光的化学传感器 已开发了用于检测PFA的调制，其中大多数仍然远离 足以进行有关灵敏度（vs. 70 ppt）或选择性的饮用水分析（反对 普通化学物质，尤其是洗涤剂）。 该项目旨在通过开发一个小型且 易于使用，提供敏感和选择性的内场检测PFO和PFOA（选择为 代表性PFA分析物）。传感器平台基于高度敏感和选择性 覆盖在固相提取（SPE）上的荧光传感器能够预召集 低浓度的分析，从而降低了检测极限。结合 在一个平台（即F-SPE）中SPE和荧光检测的预浓缩将 大大简化并加快了分析过程。而且，F-SPE采用 SPE固有的可忽略不计（ND），这将进一步简化分析过程 消除需要精确仪表常规所需的样品量的需求 分析方法。 nd依赖于将最小的样品通过膜的传递量 分析物提取达到平衡。此时，样品中的分析物浓度 进入和退出膜是相等的。结果，分析物的表面浓度 可以与样品中的浓度直接相关，但不再取决于 样品的体积通过膜。因此，不再需要 仪表通过膜的精确样品体积。此处的主要创新在于 通过预浓缩整合荧光传感器的高灵敏度和选择性 SPE的能力和ND原理，它将在简单的， 低成本的方式。 该项目将围绕三个具体目标实施： 特定目标1。选择对任何PFO的荧光团的合成和表面固定 或PFOA到内部制造的SPE磁盘。 特定目标2。对PFO和PFOA检测的F-SPE/ND评估。 特定目的3。商业化评估。