Dynamic single-cell analysis instrument to evaluate immune cell function
动态单细胞分析仪评估免疫细胞功能
基本信息
- 批准号:10699036
- 负责人:
- 金额:$ 32.43万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-05-01 至 2024-07-31
- 项目状态:已结题
- 来源:
- 关键词:AcademiaAdoptionAlgorithmsAntibodiesApoptosisAreaArtificial IntelligenceBasic ScienceBehaviorBiological AssayBiological ProductsBiologyCell CommunicationCell TherapyCell physiologyCellsCellular MorphologyCellular biologyCharacteristicsClassificationCommunicable DiseasesComputer softwareCytometryData AnalysesDetectionDevelopmentDisciplineDiseaseEffector CellElementsFlow CytometryFrequenciesGenomicsHeterogeneityImageImmuneIndividualIndustryInstitutionLabelLaboratoriesLettersLinkManufacturerMeasuresMedicalMicroscopyModalityModelingMolecular ProfilingMovementNetwork-basedNoiseNuclear TranslocationOncologyOrganellesOutputPeer ReviewPerformancePharmaceutical PreparationsPhasePropertyProtein AnalysisProtein SecretionProtein translocationProteinsPublicationsReagentResearchResearch ContractsResearch PersonnelResolutionScienceScientistService delivery modelSignal TransductionSoftware ToolsSupervisionSynapsesT-LymphocyteTechniquesTechnologyTestingTherapeuticTherapeutic antibodiesTimeTrainingTubeVaccinesartificial intelligence algorithmbiopharmaceutical industrycell behaviorcell motilitycell typeclinical biomarkersclinical developmentcluster computingcommercial applicationcommercializationcytokinecytotoxicitydesignexperimental studyflexibilityimage processingimprovedindividual variationinstrumentinstrumentationinterestlive cell imagingmanufacturemanufacturing facilitymicroscopic imagingmigrationnanoneural networknovel therapeuticspreclinical developmentprogramsprototypesingle cell analysissingle cell sequencingsingle-cell RNA sequencingspatiotemporalsuccesstechnology developmenttranscriptomicstrenduser-friendly
项目摘要
7. Project Summary/Abstract
Recent decades have been marked by a revolution in single-cell analytical techniques, instruments and
software tools that have markedly improved our understanding of biology. A high-profile example has been the
emergence of single-cell sequencing to move from bulk genomic and transcriptomic analysis to single-cell
resolution that reveals the heterogeneity of individual cells. Similar trends have been observed in protein
analysis, spatial transcriptomics, and other disciplines.
However, there remains a critical unmet need in understanding individual cellular function, movement,
interactions, and other measures of performance. The genesis of cells as living drugs requires the
development of technologies that can characterize the function and performance of massive numbers of cells
at single-cell resolution to support development of new therapies and clinical biomarkers. Unfortunately,
existing technologies are either not scalable, are cell-destructive and so cannot evaluate cells and their
interactions over time, or do not provide single-cell resolution.
CellChorus® evaluates the performance and interactions of thousands of individual cells by applying Time-
lapse Imaging Microscopy in Nanowell Grids (TIMING™) with neural network-based artificial intelligence (AI) to
identify, track, and characterize behaviors and interactions of disease cells together with T-cells and other
effector cells. The platform has been validated technically and commercially in an academic setting and an
early access laboratory, but this model does not scale.
We will develop and rigorously validate a Chronos™ instrument that delivers dynamic single-cell analysis
based on the TIMING platform and applying our existing AI software and algorithms. The Chronos instrument
will allow scientists, researchers, and manufacturing experts to apply dynamic single-cell analysis to catalyze
development and manufacture of novel therapies in infectious diseases, oncology, and other medical sciences.
7.项目总结/摘要
近几十年来,单细胞分析技术、仪器和方法发生了革命性的变化。
这些软件工具显著提高了我们对生物学的理解。一个引人注目的例子是,
单细胞测序的出现,从批量基因组和转录组分析转向单细胞测序
分辨率显示单个细胞的异质性。在蛋白质中也观察到类似的趋势
分析,空间转录组学和其他学科。
然而,在理解个体细胞功能、运动、
互动,以及其他绩效指标。细胞作为活体药物的起源需要
开发能够表征大量细胞的功能和性能的技术
以单细胞分辨率支持新疗法和临床生物标志物的开发。不幸的是,
现有的技术要么是不可扩展的,要么是细胞破坏性的,因此不能评估细胞及其
随着时间的推移的相互作用,或不提供单细胞分辨率。
CellChorus®通过应用Time-
基于神经网络的人工智能(AI)的纳米网格(TIMING™)中的延时成像显微镜,
识别、跟踪和表征疾病细胞与T细胞和其他细胞的行为和相互作用
效应细胞该平台已在学术环境中进行了技术和商业验证,
抢先体验实验室,但这种模式不成规模。
我们将开发并严格验证Chronos™仪器,该仪器可提供动态单细胞分析
基于TIMING平台,应用我们现有的AI软件和算法。Chronos仪器
将允许科学家,研究人员和制造专家应用动态单细胞分析来催化
开发和制造传染病、肿瘤学和其他医学科学的新型疗法。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Laurence J.N. Cooper其他文献
Incidence and Outcome of Early Hospital Readmission Following Hematopoetic Stem Cell Transplantation in Pediatric and Young Adult Patients
- DOI:
10.1016/j.bbmt.2014.11.391 - 发表时间:
2015-02-01 - 期刊:
- 影响因子:
- 作者:
Ossama Maher;Jorge Galvez Silva;Chloe Tillman;Demetrios Petropoulos;Laurence J.N. Cooper;Dean Lee;Laura L. Worth;Richard E. Champlin;Nidale Tarek;Priti Tewari - 通讯作者:
Priti Tewari
Evaluating the Effector Function of Individual CD19-Specific T Cells to Assess the Therapeutic Impact of a Manufactured Product
- DOI:
10.1016/j.bbmt.2013.12.207 - 发表时间:
2014-02-01 - 期刊:
- 影响因子:
- 作者:
Harjeet Singh;Ivan Liadi;Gabrielle Romain;Navin Varadarajan;Laurence J.N. Cooper - 通讯作者:
Laurence J.N. Cooper
Dual-Specificity CAR+ T Cells to Target B-Cell Malignancies and Opportunistic Fungal Infection
- DOI:
10.1016/j.bbmt.2013.12.202 - 发表时间:
2014-02-01 - 期刊:
- 影响因子:
- 作者:
Pappanaicken R. Kumaresan;Pallavi R. Manuri;Nathaniel D. Albert;Harjeet Singh;Brain Rabinovich;Janani Krishnamurthy;Sourindra N. Maiti;Olivares Simon;Tiejuan Mi;Dean Lee;Dimitrios Kontoyiannis;Helen Huls;Laurence J.N. Cooper - 通讯作者:
Laurence J.N. Cooper
Automated Production of Clinical-Grade CMV-Specific T Cells to Implement Immunotherapy at the Bedside
- DOI:
10.1016/j.bbmt.2013.12.209 - 发表时间:
2014-02-01 - 期刊:
- 影响因子:
- 作者:
Priti Tewari;Pappanaicken R. Kumaresan;Matthew Figliola;Helen Huls;Kevin Longin;Katharina Ruhnke;Richard E. Champlin;Laurence J.N. Cooper - 通讯作者:
Laurence J.N. Cooper
Reconstitution of Lymphocyte Subsets and Outcomes After Matched and Mismatched Hematopoietic Stem-Cell Transplantation
- DOI:
10.1016/j.bbmt.2012.11.409 - 发表时间:
2013-02-01 - 期刊:
- 影响因子:
- 作者:
Antonio di Stasi;Michelle Poon;Amir Hamdi;Hila Shaim;Susan Xie;Denai Milton;Roland Bassett;Gabriela Rondon;Elizabeth J. Shpall;Laurence J.N. Cooper;Dean A. Lee;Katayoun Rezvani;Richard E. Champlin;Stefan O. Ciurea - 通讯作者:
Stefan O. Ciurea
Laurence J.N. Cooper的其他文献
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{{ truncateString('Laurence J.N. Cooper', 18)}}的其他基金
Phase 1 Study of Umbilical Cord Blood-Derived T Cells in Malignant B Cells
恶性 B 细胞中脐带血衍生 T 细胞的 1 期研究
- 批准号:
8732611 - 财政年份:2013
- 资助金额:
$ 32.43万 - 项目类别:
Phase 1 Study of Umbilical Cord Blood-Derived T Cells in Malignant B Cells
恶性 B 细胞中脐带血衍生 T 细胞的 1 期研究
- 批准号:
8417456 - 财政年份:2013
- 资助金额:
$ 32.43万 - 项目类别:
Quantitative single-cell biomarkers of T-cells to optimize tumor immunotherapy
T 细胞的定量单细胞生物标志物可优化肿瘤免疫治疗
- 批准号:
8413987 - 财政年份:2012
- 资助金额:
$ 32.43万 - 项目类别:
IMAGING T CELLS BY POSITRON EMISSION TOMOGRAPHY
通过正电子发射断层扫描对 T 细胞进行成像
- 批准号:
8373689 - 财政年份:2012
- 资助金额:
$ 32.43万 - 项目类别:
Quantitative single-cell biomarkers of T-cells to optimize tumor immunotherapy
T 细胞的定量单细胞生物标志物可优化肿瘤免疫治疗
- 批准号:
8547802 - 财政年份:2012
- 资助金额:
$ 32.43万 - 项目类别:
IMAGING T CELLS BY POSITRON EMISSION TOMOGRAPHY
通过正电子发射断层扫描对 T 细胞进行成像
- 批准号:
8539750 - 财政年份:2012
- 资助金额:
$ 32.43万 - 项目类别:
IMAGING T CELLS BY POSITRON EMISSION TOMOGRAPHY
通过正电子发射断层扫描对 T 细胞进行成像
- 批准号:
8711377 - 财政年份:2012
- 资助金额:
$ 32.43万 - 项目类别:
T-cell Therapy for B-lineage Acute Lymphoblastic Leukemia
B 系急性淋巴细胞白血病的 T 细胞疗法
- 批准号:
8681381 - 财政年份:2010
- 资助金额:
$ 32.43万 - 项目类别:
T-cell Therapy for B-lineage Acute Lymphoblastic Leukemia
B 系急性淋巴细胞白血病的 T 细胞疗法
- 批准号:
8112556 - 财政年份:2010
- 资助金额:
$ 32.43万 - 项目类别:
nCounter Prep Station and the Digital Analyzer
nCounter Prep Station 和数字分析仪
- 批准号:
7793214 - 财政年份:2010
- 资助金额:
$ 32.43万 - 项目类别:
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