DCEG- Molecular Assays for epidemiology studies

在这个项目中进行的活动包括为癌症研究提供人类流行病学标本的DNA提取、alliquoting和生物处理。一些设施被用来处理人类标本，并提供必要的基础设施，以支持人类标本的处理和储存，以支持流行病学癌症研究。质谱中心配备了最新的质谱（MS）仪器，用于分析小蛋白质和大蛋白质生物分子，包括内源性激素。该中心在检测、定量、结构确认和质量测量方面提供相应的专业知识。该中心还教育科学家使用、解释和应用质谱技术。clia认证的分子诊断实验室提供最先进的临床和研究诊断，包括突变检测、单核苷酸多态性分析和病原体检测。人类免疫监测实验室通过检查在病毒相关肿瘤的发病机制或预防中可能重要的人类宿主免疫参数来支持宫颈癌研究。该实验室的能力包括：接收和处理人体标本，包括全血和宫颈标本；外周血单个核细胞（PBMC）的低温保存、DNA提取和eb病毒转化；免疫学检测，包括各种体外PBMC功能检测；流式细胞术（荧光活化细胞分选- FACS）分型HLA和PBMC；临时低温保存生物标本并转移至DCEG生物库；对生物标本进行电子跟踪，并向DCEG调查人员报告分析结果；维护质量控制/质量保证程序；尽可能使用最先进的技术。生物处理实验室对生物标本进行处理，然后将其储存在DCEG储存库或分发给合作者。处理实验室提供：全血、血清、血浆、尿液、粪便等生物标本的处理和冷冻；标本冻干；组织在液氮中快速冷冻；细胞系的分离和冷冻保存，包括常规的生存能力评估；低温保存红细胞以维持酶活性；细胞系的建立；细胞系的大规模繁殖；核酸提取；标本分布。DNA提取实验室是CGR的一部分，从全血标本、血液组分、转化淋巴母细胞系、培养成纤维细胞、肿瘤标本、颊细胞、石蜡包埋组织、格思里卡、细胞学制剂或其他生物标本中提取DNA。在这个项目中进行的活动包括为癌症研究提供人类流行病学标本的DNA提取、alliquoting和生物处理。

Activities performed within this project provide DNA extraction, alliquoting, bioprocessing of human epidemiology specimens for cancer research. Several facilities are utilized to process human specimens and to provide the necessary infrastructure to support human specimen processing and storage in support of epidemiology cancer research. The Mass Spectrometry Center is equipped with the latest in mass spectrometry (MS) instrumentation for analyzing both small and large protein biomolecules, including endogenous hormones. The Center offers corresponding expertise in detection, quantitation, structure confirmation, and mass measurements. The Center also educates scientists on the use, interpretation, and applications of MS technologies. The CLIA-certified Molecular Diagnostics Laboratory provides state-of-the-art clinical and research diagnostics, including mutation detection, single nucleotide polymorphism analysis, and pathogen detection. The Human Immunological Monitoring Laboratory supports cervical cancer research by examining human host immunological parameters potentially important in the pathogenesis or prevention of virus-related tumors. The laboratory's capabilities include: receipt and processing of human specimens, including whole blood and cervical specimens; cryopreservation, DNA extraction and Epstein-Barr virus transformation of peripheral blood mononuclear cells (PBMC); immunological testing, including a variety of in vitro PBMC functional assays; HLA and PBMC phenotyping by flow cytometry (fluorescence activated cell sorting – FACS); temporary low temperature storage of biological specimens and transfer to the DCEG biorepository; electronic tracking of biospecimens and reporting of assay results to DCEG investigators; maintain quality control/quality assurance programs; apply state-of-the-art techniques, as they become available. The Bioprocessing Laboratory processes biological specimens prior to their storage in the DCEG repository or dispersal to collaborators. The processing laboratory provides: processing and freezing of biological specimens, including whole blood, serum, plasma, urine, and feces; lyophilization of specimens; flash-freezing of tissue in liquid nitrogen; separation and viable cryopreservation of cell lines, including the routine assessment of viability; cryopreservation of red blood cells to maintain enzymatic activity; establishment of cell lines; large scale propagation of cell lines; nucleic acid extraction; distribution of specimens. The DNA Extraction Laboratory, a part of the CGR, extracts DNA from whole blood specimens, blood fractions, transformed lymphoblastic lines, cultured fibroblasts, tumor specimens, buccal cells, paraffin-embedded tissue, Guthrie cards, cytology preparations, or other biological specimens. Activities performed within this project provide DNA extraction, alliquoting, bioprocessing of human epidemiology specimens for cancer research.