Pulmonary Hypertension in Left Heart Disease

左心病肺动脉高压

• 批准号: 10656879
• 负责人: Barry A. Borlaug
• 金额: $ 80.35万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2028-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10656879
• 关键词: Address; Adult; Aerobic; Anatomy; Animal Model; Autopsy; Back; Bioinformatics; Biological; Biology; Blood Vessels; Blood capillaries; Circulation; Classification; Clinical; Data; Disease; Epidemiology; Exertion; FRAP1 gene; Functional disorder; Glean; Goals; Heart Diseases; Histologic; Human; Knowledge; Left; Life Expectancy; Link; Localized Disease; Location; Lung; Lung diseases; Mass Spectrum Analysis; Mediating; Miniature Swine; Morbidity - disease rate; Nature; Neonatal; Outcome Study; Outcomes Research; Pathway interactions; Patients; Phenotype; Prognosis; Proteins; Proteomics; Pulmonary Heart Disease; Pulmonary Hypertension; Pulmonary Vascular Resistance; Pulmonary arterial remodeling; Research; Rho-associated kinase; Severities; Signal Transduction; Sirolimus; Structure of parenchyma of lung; Symptoms; System; Techniques; Terminology; Therapeutic; Therapeutic Agents; Vascular remodeling; Venous; Ventricular; Work; differential expression; effective therapy; fasudil; hemodynamics; individualized medicine; innovation; kinase inhibitor; laser capture microdissection; mortality; novel; novel strategies; novel therapeutics; porcine model; pressure; programs; pulmonary arterial hypertension; pulmonary arterial pressure; pulmonary vascular disorder; pulmonary venous hypertension; response; right ventricular failure; stem; symptomatic improvement; therapeutic target; transcriptomics; treatment response; vasoconstriction

PROJECT SUMMARY / ABSTRACT Left heart disease (LHD) leads to pulmonary hypertension (PH-LHD, aka Group 2 PH), right ventricular (RV) failure, and increased mortality and morbidity. Advances in pulmonary vascular biology gleaned from study of the pulmonary arterial (PA) circulation in Group 1 PH and relevant animal models have led to effective therapies for Group 1 PH. Trials of Group 1 PH therapies in PH-LHD have shown highly variable (favorable, neutral or harmful) effects. We propose that two critical knowledge gaps contribute to variability in therapeutic response and impede progress in treating PH-LHD: (1) the lack of a mechanistically informative hemodynamic classification system defining the nature (vasoconstriction vs remodeling) and location (PA vs pulmonary venous (PV)) of pulmonary vascular disease in LHD, and (2) lack of understanding of vessel specific (PV vs PA) biological pathways mediating pulmonary vascular disease in PH-LHD. The objective of this proposal is to address these knowledge gaps and enable therapeutic innovation in PH-LHD. Based on extensive preliminary studies in human and experimental (Exp) PH-LHD, our central hypothesis is that PH-LHD is a phenotypically diverse entity whose ultimate therapeutic approach will be defined by unique hemodynamic phenogroups and vessel specific (PA vs PV) pathophysiological perturbations. In human and Exp PH-LHD, we will use novel hemodynamic assessments to phenotype PH-LHD according to pulmonary vascular resistance (PVR), vasoreactivity, and the longitudinal distribution of PVR (Aim 1). Findings will be validated in human PH-LHD by assessing phenogroup-specific differences in aerobic capacity, RV reserve function and exertional lung congestion. Findings in Exp PH-LHD will be validated by defining PA and PV remodeling (quantitative histomorphometry). Our broad hypothesis is that both the primary mechanism and location of the elevated PVR in PH-LHD have clinical implications and anatomical underpinnings. In human and Exp PH-LHD, we will then (Aim 2) use histochemical, proteomic, and transcriptomic based techniques and bioinformatic analyses to define vessel specific mechanisms across PH-LHD phenogroups. These studies will couple the Aim 1 hemodynamic phenotyping approach to vessel specific vascular biology. In Aim 3, we will determine if therapeutic agents based on our omics studies in human and Exp PH-LHD will ameliorate PV or PA remodeling and delay the progression of PH in Early or Late Exp PH-LHD phenogroups. The research outcome from this work will be a new hemodynamic classification of PH-LHD linked to specific pathophysiology and therapeutic targets, thus enabling individualized medicine approaches to PH-LHD.

项目总结/摘要 左心疾病（LHD）导致肺动脉高压（PH-LHD，又名第2组PH）、右心室（RV） 失败，并增加死亡率和发病率。肺血管生物学研究进展 第1组PH和相关动物模型中的肺动脉（PA）循环导致了有效的治疗方法 在PH-LHD中进行的第1组PH治疗试验显示出高度可变（有利、中性或 有害的）影响。我们认为，两个关键的知识差距有助于治疗反应的变异性 并阻碍了PH-LHD治疗的进展：（1）缺乏机械信息的血流动力学 定义性质（血管收缩与重塑）和位置（PA与肺静脉）的分类系统 (PV))LHD中肺血管疾病的发生率，以及（2）缺乏对血管特异性（PV vs PA）的了解 PH-LHD中介导肺血管疾病的生物学途径。这项建议的目的是 解决这些知识差距，并实现PH-LHD的治疗创新。根据广泛的初步 在人类和实验性（Exp）PH-LHD研究中，我们的中心假设是PH-LHD是一种表型 不同的实体，其最终治疗方法将由独特的血流动力学表型定义， 血管特异性（PA vs PV）病理生理学扰动。在人类和实验PH-LHD中，我们将使用新的 根据肺血管阻力（PVR）对表型PH-LHD进行血流动力学评估， 血管反应性和PVR的纵向分布（目的1）。将通过以下方法在人PH-LHD中验证结果： 评估有氧能力、RV储备功能和劳力肺的表型组特异性差异 拥堵将通过定义PA和PV重塑（定量）来验证Exp PH-LHD中的结果 组织形态测定法）。我们广泛的假设是，PVR升高的主要机制和位置 具有临床意义和解剖学基础。在人类和实验性PH-LHD中，我们将 (Aim 2）使用基于组织化学、蛋白质组学和转录组学的技术和生物信息学分析来定义 跨PH-LHD表型组的血管特异性机制。这些研究将结合Aim 1血流动力学 血管特异性血管生物学的表型分析方法。在目标3中，我们将确定是否基于 在我们的人类组学研究中，实验PH-LHD将改善PV或PA重塑并延缓进展， 在早期或晚期Exp PH-LHD表型组中的PH。这项工作的研究成果将是一个新的 PH-LHD的血流动力学分类与特定的病理生理学和治疗靶点相关，因此能够 PH-LHD的个体化医学方法。