Nutrition, Exercise and phenotype Testing Core

摘要/总结 – NExT 核心 MNORC 营养、运动和表型测试 (NExT) 核心的总体目标 （原“人类表型核心”（HPC）旨在加强和扩展研究 MNORC 研究人员进行相关临床和转化研究的能力 人类的营养、肥胖和/或肥胖相关疾病。 NExT 核心实现了这一点 目标： 1) 为转化、临床和人群提供咨询服务 研究者对其进行适当的评估、测试和/或干预方法 与营养、体力活动/锻炼、肥胖或肥胖相关的有氧运动相关的研究 代谢紊乱，2）为复杂和标准化提供专业知识和基础设施 对人类受试者（包括儿童和成人）进行生理测试 3) 提供服务 直接协助研究人员进行营养和/或身体活动/锻炼 对人类受试者的干预，以及 4) 协助开发新技术或 获得人类代谢表型分析的新技术。除了提供广泛的产品系列 相对“标准”的表型测量（例如人体测量和身体成分 （DXA、PeaPod）、血压、空腹血液检查、口服葡萄糖耐量、营养和 通过自我报告和客观方法（测试膳食、 加速度计）和有氧健身评估）NExT 核心还提供更多功能 涉及更彻底评估代谢表型的程序（例如， 高胰岛素-正常血糖钳夹、同位素示踪代谢研究、静息能量 支出、骨骼肌和脂肪组织活检）。在开始学习之前， MNORC 调查人员可以咨询 NExT 核心专家专家，以深入了解如何 可用的方法可以帮助他们检验他们的假设，并有可能扩展他们的研究 使用通过核心提供给他们的工具来研究问题。下一个核心 工作人员还咨询 MNORC 研究人员，以帮助优化他们的研究设计和研究 研究干预策略/方法。 NEXT核心也直接协助MNORC 研究人员通过提供以下服务来进行肥胖/营养干预： 与研究参与者进行营养咨询，监督运动训练干预措施， 干预期间身体活动和饮食行为的评估，以及 协助进行专门针对每个研究参与者的干预措施。 总体而言，NExT 核心的服务提供了良好控制和标准化的表型 测试、“更深入”表型评估的最先进技术以及帮助 进行严格控制的研究干预，所有这些都极大地增强和扩展了 研究人员在儿童营养和肥胖领域使用的现有方法 成人。

Abstract/Summary – NExT Core The overall objective of the Nutrition, Exercise and phenotype Testing (NExT) core of MNORC (formerly the “Human Phenotyping Core” (HPC) is to enhance and expand the research capabilities of MNORC investigators performing clinical and translational studies related to nutrition, obesity, and/or obesity-related disorders in humans. The NExT core accomplishes this goal by: 1) Provide consultation services to translational, clinical and population-based investigators regarding appropriate assessment, testing and/or intervention approaches for their studies related to nutrition, physical activity/exercise, obesity, or obesity-related cardio- metabolic disorders, 2) offering expertise and infrastructure for sophisticated and standardized physiologic testing of human subjects, including children and adults 3) providing services to directly assist researchers to conduct their nutritional and/or physical activity/exercise interventions in human subjects, and 4) assisting in the development of new techniques or acquire new technologies for human metabolic phenotyping. In addition to offering a wide array of relatively “standard” phenotypic measurements (e.g., anthropometry and body composition (DXA, PeaPod), blood pressure, fasting blood work, oral glucose tolerance, nutritional and physical activity assessments via self reports and objective methods (test meals, accelerometry), and aerobic fitness assessments) the NExT core also offers several more involved procedures to more thoroughly assess the metabolic phenotype (e.g., hyperinsulinemic-euglycemic clamp, isotope-tracer metabolic studies, resting energy expenditure, skeletal muscle and adipose tissue biopsies). Before beginning their studies, MNORC investigators may consult with expert NExT core personnel to gain insights on how the available methodologies may help them to test their hypotheses, and potentially expand their research questions using the tools made available to them through the Core. NExT core personnel also consult with MNORC investigators to help optimize their study designs and their research intervention strategies/approaches. The NEXT core also directly assists MNORC investigators to conduct their obesity/nutritional interventions by providing services such as: nutritional consultations with study participants, supervision of exercise training interventions, assessment of physical activity and dietary behaviors during an intervention, as well as assistance with conducting interventions specifically tailored to each research participant. Overall, the services of the NExT core provide well-controlled and standardized phenotypic testing, state-of-the-art techniques for “deeper” phenotype assessment, as well as assistance to conduct well-controlled research interventions, all of which greatly enhance and expand on existing approaches used by researchers in the areas of nutrition and obesity in children and adults.