UT Southwestern NORC

项目摘要/摘要--概述： UTSW NORC的总体目标是通过以下方式加强营养与肥胖的研究努力 促进跨学科互动，加快将基本科学发现转化为临床相关成果 对人类的干预，改善公共健康。我们已经召集了一个高度互动和协作的 德克萨斯大学西南分校的跨学科调查团队利用了不同学科的专业知识， 目标是确定导致肥胖的行为、代谢、遗传和分子机制 以及肥胖引发的疾病。 UTSW NORC研究基地由来自27个不同部门的158名调查人员组成。这些 调查人员有230笔外部资助的营养/肥胖相关研究拨款，总额超过74美元 百万/年的直接成本。为了促进跨学科研究和思想交流，我们的NORC 创建了以下主题相关的团队：团队1：能量代谢的中央调节；团队2： 脂肪细胞生物学和能量代谢；第三组：肥胖引起的外周器官疾病；第四组： 营养、肥胖和癌症。在每个团队中，我们都汇集了来自不同领域的科学家 全面解决肥胖的生化、代谢和临床后果的学科 相关疾病。每个团队都有高级、中级和初级科学家，这将增强 培养初级科学家，增加成功合作的可能性。P&F计划将 每年支持四名新调查人员，他们也将被分配到四个研究小组中的一个。 为了支持我们的研究工作，我们建立了四个核心和一个临床元素，以提供最新的 超出NORC调查人员个人能力、可用性和/或预算的ART服务。这个 动物表型/代谢核心提供广泛的血液代谢物和激素阵列，50 代谢笼子，血压、心率、核心体温和活动的遥测测量， 多种成像方式来表征脂肪分布，以及高胰岛素-正常血糖钳夹和 原代细胞分离。脂质谱核心提供全面的脂类定量。 细胞、组织和血液。定量新陈代谢和成像核心提供复杂的通量 体内代谢途径的测定及骨骼肌的~1H成像和光谱分析 和人类肝脏中的脂肪含量。遗传学、单细胞测序和RNA序列核心提供外显子 测序和基因表达与分析。所有核心都为动物和人类研究提供服务， 支持更快地将发现从动物模型转化为人类可验证假说的能力。 UTSW NORC为一大群不同的调查人员提供了一个重要的联合机制 并集中他们与营养和肥胖相关的研究努力，以加快基础科学的翻译 对人类临床相关干预措施的发现，以改善公共健康。

PROJECT SUMMARY/ABSTRACT – Overview: The overarching goal of the UTSW NORC is to enhance research efforts in nutrition and obesity by facilitating interdisciplinary interactions to speed translation of basic scientific discoveries into clinically relevant interventions in humans that improve public health. We have assembled a highly interactive and collaborative interdisciplinary team of investigators at UT Southwestern that harnesses expertise from diverse disciplines, with a goal of defining the behavioral, metabolic, genetic, and molecular mechanisms contributing to obesity and obesity-induced disease. The UTSW NORC research base consists of 158 investigators from 27 different Departments. These investigators have 230 extramurally funded nutrition/obesity-related research grants that total more than $74 million/yr in direct costs. To facilitate interdisciplinary research and the exchange of ideas, our NORC has created the following thematically related Teams: Team 1: Central Regulation of Energy Metabolism; Team 2: Adipocyte Biology and Energy Metabolism; Team 3: Obesity-induced Peripheral Organ Disease; and Team 4: Nutrition, Obesity, and Cancer. Within each team, we have integrated scientists from a wide spectrum of disciplines to comprehensively address the biochemical, metabolic, and clinical consequences of obesity and related diseases. Each Team has a mixture of senior, mid-level, and junior scientists, which will enhance the development of junior scientists and increase the likelihood of successful collaborations. The P&F program will support four new investigators/year who will also be assigned to one of the four research Teams. To support our research efforts, we have established four cores and a Clinical Element to provide state-of- the art services that exceed the capabilities, availability and/or budgets of individual NORC investigators. The Animal Phenotyping/Metabolism Core provides an extensive array of blood metabolites and hormones, 50+ metabolic cages, telemetry measurements of blood pressure, heart rate, core body temperature, and activity, multiple imaging modalities to characterize fat distribution, and hyperinsulinemic-euglycemic clamps and primary cell isolation. The Lipid Mass Spectrometry Core provides comprehensive quantification of lipids in cells, tissues, and blood. The Quantitative Metabolism and Imaging Core provides sophisticated flux measurements of metabolic pathways in vivo and 1H imaging and spectroscopy to characterize skeletal muscle and liver lipid content in humans. The Genetics, Single Cell Sequencing & RNA seq core provides exome sequencing and gene expression and analysis. All cores provide services for animal and human research, supporting the ability to translate findings more rapidly from animal models into testable hypothesis in humans. The UTSW NORC provides an important mechanism for a large group of diverse investigators to coalesce and focus their research efforts related to nutrition and obesity to accelerate translation of basic scientific discoveries into clinically relevant interventions in humans to improve public health.