Evaluating Mechanisms and Therapeutic Potential of (GLP-1R) Agonists for Glaucoma Treatment

评估 (GLP-1R) 激动剂治疗青光眼的机制和治疗潜力

• 批准号: 10660124
• 负责人: Qi N Cui
• 金额: $ 51.33万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-05 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10660124
• 关键词: Address; Agonist; Antiinflammatory Effect; Astrocytes; Automobile Driving; Blindness; Body Weight decreased; Brain; Cell Death; Cell Survival; Cell physiology; Cells; Chronic; Circulation; Clinical Trials; Complement 1q; Complement 3; Cytoprotection; Data; Diabetes Mellitus; Disease; Disease Progression; Eye; FDA approved; Fluorescein-5-isothiocyanate; GLP-I receptor; Generations; Genetic; Glaucoma; Human; Immunohistochemistry; In Situ Hybridization; In Vitro; Infiltration; Inflammation; Inflammatory Response; Inherited; Interleukin-1 alpha; Knockout Mice; Knowledge; Macrophage; Macrophage Activation; Maps; Measures; Mediating; Mediator; Microglia; Microspheres; Modality; Modeling; Mus; Myeloid Cells; Nature; Nerve Degeneration; Neurodegenerative Disorders; Neurons; Newly Diagnosed; Non-Insulin-Dependent Diabetes Mellitus; Ocular Hypertension; Optic Disk; Parkinson Disease; Pathogenesis; Patients; Phenotype; Physiologic Intraocular Pressure; Process; Production; Receptor Activation; Retina; Retinal Ganglion Cells; Risk; Signal Transduction; TNF gene; Testing; Therapeutic; Translations; astrogliosis; cell type; cytokine; diabetic patient; dopaminergic neuron; experimental study; fighting; hazard; hypertensive; in vivo; insurance claims; intravitreal injection; mimetics; mouse model; neural; neuroinflammation; neuron loss; neuronal cell body; neuroprotection; novel; novel therapeutics; preservation; prevent; progressive neurodegeneration; receptor expression; response; retinal ganglion cell degeneration; symptomatic improvement; targeted treatment; tool; treatment strategy

Project Summary Glaucoma is characterized by retinal ganglion cell (RGC) death leading to vision loss. Available treatment modalities continue to rely on intraocular pressure (IOP) reduction, which is insufficient to prevent progressive neurodegeneration in a significant number of glaucoma patients. In the fight against this blinding disease, treatment strategies that do not rely on IOP-lowering are urgently needed. In this proposal, we hypothesize that glucagon-like peptide-1 receptor (GLP-1R) agonists protect against glaucomatous neurodegeneration by decreasing microglia/macrophage activation and retinal macrophage infiltration, in turn preventing reactive astrogliosis resulting in RGC rescue. This hypothesis builds upon our prior study showing that induced ocular hypertension in a mouse model of glaucoma triggers microglia/macrophage activation and reactive astrocyte formation in the retina. We found that treatment with the long-acting GLP-1R agonist NLY01 suppressed microglia/macrophage activation, prevented reactive astrogliosis, and rescued RGCs following IOP elevation. Further, our examination of insurance claims data showed that treatment with GLP-1R agonists, FDA-approved to treat diabetes and for weight loss, is associated with decreased glaucoma risk in humans. However, the retinal cell type(s) mediating GLP-1R agonists' RGC protection have not been identified. Further, it is not known whether systemic macrophage infiltration and/or resident microglia transformation drive early inflammation, and whether NLY01 modifies this response. Finally, whether this favorable response to NLY01 treatment generalizes beyond induced IOP elevation to inherited models of chronic, progressive glaucoma is unknown. This proposal will pursue 2 specific aims crucial to evaluating GLP-1R agonists' mechanism of action and the potential GLP-1R agonists hold as novel glaucoma therapy: 1) Determine the mechanisms through which the GLP-1R agonist NLY01 rescues RGCs following IOP elevation, and 2) Determine the mechanisms of GLP-1R agonist-mediated neural rescue in an inherited model of glaucoma. Findings will determine: 1) the systemic cell type(s) facilitating NLY01's RGC rescue, including whether macrophage infiltration drives early inflammation in response to ocular hypertension, and 2) whether the GLP-1R agonist NLY01 exerts a long-term anti-inflammatory effect to rescue RGCs in the DBA/2J mouse model of glaucoma. This proposal is the first step in a broader plan to disentangle systemic effects of GLP-1R activation driving neuronal rescue. Results will serve to advance our understanding of glaucoma pathogenesis, identify the mechanisms driving NLY01-mediated RGC rescue, and elucidate the potential for using GLP-1R agonists in glaucoma treatment.

项目摘要 青光眼的特征是视网膜神经节细胞(RGC)死亡导致视力丧失。可用的治疗方法 治疗方式继续依赖于降低眼压，这不足以防止渐进性 相当数量的青光眼患者出现神经变性。在与这种致盲疾病的斗争中， 迫切需要不依赖于降低眼压的治疗策略。在这项提案中，我们假设 胰升糖素样肽-1受体(GLP-1R)激动剂通过以下途径预防青光眼神经变性 减少小胶质细胞/巨噬细胞的激活和视网膜巨噬细胞的渗透，进而阻止反应性 星形胶质细胞增生症导致RGC抢救。这一假说建立在我们之前的研究基础上，该研究表明诱导眼 小鼠青光眼模型中的高血压触发小胶质细胞/巨噬细胞激活和反应性星形胶质细胞 在视网膜上形成。我们发现，长效GLP-1R激动剂NLY01的治疗抑制了 激活小胶质细胞/巨噬细胞，防止反应性星形胶质细胞增生，并在眼压升高后挽救视网膜节细胞。 此外，我们对保险索赔数据的检查显示，FDA批准的GLP-1R激动剂治疗 治疗糖尿病和减肥，与降低人类患青光眼的风险有关。然而，视网膜 介导GLP-1R激动剂RGC保护作用的细胞类型(S)尚未确定。此外，目前还不知道 全身性巨噬细胞浸润和/或常驻小胶质细胞转化推动早期炎症，以及 NLY01修改此响应。最后，这种对NLY01治疗的有利反应是否超出了 遗传性慢性进行性青光眼模型引起的眼压升高尚不清楚。这项提议将 追求两个对评估GLP-1R激动剂的作用机制和潜在的GLP-1R至关重要的特定目标 作为青光眼新疗法的激动剂：1)确定GLP-1R激动剂的机制 NLY01对高眼压后视网膜神经节细胞的保护作用；2)确定GLP-1R激动剂介导的机制 遗传性青光眼模型的神经挽救。研究结果将确定：1)系统细胞类型(S)促进 《NLY01》S RGC救援，包括巨噬细胞浸润是否推动眼部早期炎症反应 高血压，以及2)GLP-1R激动剂NLY01是否具有长期抗炎作用 视网膜神经节细胞在DBA/2J小鼠青光眼模型中的作用这项提议是一个更广泛的解决方案的第一步。 GLP-1R激活驱动神经营救的系统效应。结果将有助于增进我们的理解 青光眼的发病机制，确定NLY01介导的RGC挽救的机制，并阐明 在青光眼治疗中使用GLP-1R激动剂的可能性。