Core E: Invertebrate Longevity and Healthspan

核心 E：无脊椎动物的长寿和健康寿命

• 批准号: 10670105
• 负责人: Alexander Richard Mendenhall
• 金额: $ 15.37万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-15 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10670105
• 关键词: Age; Aging; Animals; Area; Biochemical; Biological; Biological Assay; Biology of Aging; Biomedical Research; Caenorhabditis elegans; Cells; Chronology; Collaborations; Color; Communities; Complex; Computer Analysis; Data; Development; Devices; Disease; Education and Outreach; Fluorescence Microscopy; Funding; Goals; Histones; Hour; Individual; Invertebrates; Label; Left; Longevity; Measures; Methodology; Methods; Microfluidics; Modeling; Modification; Molecular; Mothers; Movement; Mutation; Nematoda; Nuclear Pore; Perception; Phenotype; Pilot Projects; Population; Process; Proteins; Reporter; Research; Research Personnel; Resolution; Resources; Robotics; Saccharomyces cerevisiae; Saccharomycetales; Sampling; Scheme; Services; Shock; Technology; Time; Training; Work; Yeasts; age related; aging population; behavioral phenotyping; cell age; experimental study; genetic selection; healthspan; high dimensionality; innovation; interest; learning materials; light microscopy; magnetic beads; microscopic imaging; model organism; movie; new technology; novel strategies; optical spectra; robotic system; tool

CORE E: INVERTEBRATE LONGEVITY AND HEALTHSPAN – SUMMARY/ABSTRACT This Core will assist investigators throughout the research community in quantifying a variety of longevity and healthspan measures in two of the major invertebrate model organisms: the budding yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegans. This will be accomplished through direct collaboration, training and outreach, and the development and dissemination of new tools and technologies. New technologies we have developed and will disseminate include microfluidic tools for invertebrate aging studies and the WormBot, a robotic system for high-throughput survival and behavioral phenotyping in C. elegans. We will also work to develop chemostat-based solutions for obtaining age-matched pure populations of yeast for biochemical and cell biological experiments requested by Core users. The focus on yeast and worms allows us to provide services and technologies to a large number of external investigators and thereby have a correspondingly large impact. The data generated by this Core can be correlated with high dimensional computational analyses (Core F) and with the protein and metabolite phenotypes studied in Cores C and D, respectively. Invertebrate models are also highly accessible to junior investigators, and Core B support for pilot projects allows these investigators access to the full range of integrated services provided by the Center Resource Cores.

核心E：无脊椎动物寿命和健康跨度-总结/摘要 该核心将协助整个研究界的研究人员量化各种寿命， 两种主要的无脊椎动物模式生物的健康寿命测量：芽殖酵母 酿酒酵母和线虫秀丽隐杆线虫。这将通过直接合作来实现， 培训和推广，以及开发和传播新工具和技术。新 我们已经开发并将推广的技术包括用于无脊椎动物衰老研究的微流体工具 和蠕虫机器人，一个机器人系统的高通量生存和行为表型C。优美的我们 还将致力于开发基于恒化器的解决方案，以获得年龄匹配的纯酵母菌群， 核心用户要求的生化和细胞生物学实验。对酵母和蠕虫的关注 我们向大量外部调查人员提供服务和技术，从而拥有 相应的影响很大。由该核心生成的数据可以与高维数据相关联。 计算分析（核心F）和核心C和D中研究的蛋白质和代谢物表型， 分别无脊椎动物模型对初级研究者来说也是非常容易获得的，核心B支持对试点研究者来说也是非常容易获得的。 项目使这些调查人员能够获得中心提供的全方位综合服务 资源核心。