SBIR TOPIC 107 REAGENTS FOR IMMUNOLOGIC ANALYSIS OF NON- MAMMALIAN AND UNDERREPRESENTED MAMMALIAN MODELS

SBIR 主题 107 用于非哺乳动物和代表性不足的哺乳动物模型免疫分析的试剂

• 批准号: 10688716
• 负责人: TORI RACE
• 金额: $ 59.65万
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-30 至 2024-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10688716
• 关键词: 2019-nCoV; Affinity; Cell surface; Cells; Communicable Diseases; Generations; Hamsters; Immunity; Immunization; Immunologics; Inflammatory; Influenza; Memory; Modeling; Monoclonal Antibodies; Mus; Proteins; Reagent; Recombinant Proteins; Recombinants; Regulatory T-Lymphocyte; Research Personnel; Respiratory Tract Infections; Serum; Small Business Innovation Research Grant; T memory cell; T-Cell Activation; T-Lymphocyte; Testing; Tissue Sample; Tissues; Validation; Viral Pathogenesis; chemokine; cytokine; infectious disease model; preclinical study; response; vaccine development

The hamster is a well-established model for several infectious diseases including influenza and SARS-CoV-2. Lack of hamster reagents has hampered pre-clinical studies of viral pathogenesis, host immunity, and vaccine development. The objectives of this proposal are to produce and test monoclonal antibodies against 10 cytokines and chemokines and 4 T cell surface markers for characterizing cytokine responses and examining T cells activation in the hamster models of respiratory infections. Completion of this project will provide high-affinity monoclonal antibodies that allow investigators to distinguish innate and adaptive inflammatory cytokine and chemokine responses as well as activated T cells, memory T cells, tissue resident memory T cells, and regulatory T cells in the hamster models of infectious diseases.

仓鼠是包括流感和SARS-CoV-2在内的几种传染病的成熟模型。仓鼠试剂的缺乏阻碍了对病毒发病机制、宿主免疫和疫苗开发的临床前研究。本研究的目的是制备和测试10种细胞因子和趋化因子的单抗和4种T细胞表面标志物，以确定细胞因子的反应和检测呼吸道感染模型中T细胞的激活。该项目的完成将提供高亲和力的单抗，使研究人员能够区分先天和适应性炎症细胞因子和趋化因子反应，以及在感染疾病的仓鼠模型中激活的T细胞、记忆T细胞、组织驻留记忆T细胞和调节T细胞。