Flow cytometric detection of malignant cells in body fluids

流式细胞仪检测体液中的恶性细胞

• 批准号: 10703045
• 负责人: Hao-Wei Wang
• 金额: $ 28.24万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10703045
• 关键词: B-Cell Lymphomas; Biological Assay; Body Fluids; Cell Count; Cerebrospinal Fluid; Chemistry; Clinical; Consensus; Cytology; Detection; Diagnosis; Disease; Evaluation; Flow Cytometry; Goals; HIV; Hematopoietic Neoplasms; Human Herpesvirus 8; International; Liquid substance; Lymphocyte; Lymphoma; Methodology; Molecular Diagnosis; Morphology; Multicentric Angiofollicular Lymphoid Hyperplasia; Neoplasms; Patients; Physical shape; Pleural; Process; Prognostic Factor; Protocols documentation; Rare Diseases; Risk; Role; Series; Staging; Techniques; Therapeutic; Therapeutic Intervention; Vitreous humor; cancer cell; improved; leukemia; neoplastic; primary effusion lymphoma; prognostic; symposium

Primary effusion lymphoma is an aggressive lymphoma arising in the setting of HIV and HHV8 that primarily involves body fluids. Multicentric Castlemans disease is a non-clonal proliferative process that arises in the same clinical setting. Morphological diagnosis is difficult in these rare diseases. We have developed clinical flow cytometric assays that can aid in the diagnosis of primary effusion lymphoma and multicentric Castlemans disease, providing key clinical support to patients on NCI protocols. Involvement of the cerebrospinal fluid (CSF) by hematopoietic malignancies may also be difficult to document by morphology alone. In a study comparing morphology alone to morphology with flow cytometry, we demonstrated that flow cytometric immunophneotyping was useful in establishing a diagnosis of neoplasia in a series of patients with known lymphoma or leukemia and an initial diagnosis of "atypical" or "suspicious" CSF using morphologic criteria. The Flow Cytometry Unit evaluated the role of flow cytometric analysis in staging and management of patients with high grade B cell lymphomas. Flow cytometry identified neoplastic clones that constituted as little as 0.02% of total CSF lymphocytes. Flow cytometry detected involvement where cytology, chemistry and cell counts failed. Flow cytometric detection of disease was a negative prognostic factor and now prompts therapeutic intervention. As a result of the study it is recommended that patients at risk for CNS involvement by aggressive B cell lymphoma undergo staging CSF evaluation by flow cytometry. The Flow Cytometry Unit participated in an international consensus conference to determine optimal methodology for flow cytometric analysis of CSF.

原发性渗出性淋巴瘤是一种在 HIV 和 HHV8 背景下产生的侵袭性淋巴瘤，主要涉及体液。多中心卡斯尔曼病是在同一临床环境中出现的非克隆增殖过程。这些罕见疾病的形态学诊断很困难。我们开发了临床流式细胞术检测方法，可以帮助诊断原发性渗出性淋巴瘤和多中心 Castlemans 病，为 NCI 方案的患者提供关键的临床支持。造血系统恶性肿瘤对脑脊液 (CSF) 的影响也可能难以仅通过形态学来记录。在一项单独比较形态学与流式细胞术形态学的研究中，我们证明流式细胞术免疫分型有助于对一系列已知患有淋巴瘤或白血病的患者进行肿瘤诊断，并使用形态学标准对“非典型”或“可疑”脑脊液进行初步诊断。流式细胞仪评估了流式细胞仪分析在高级别 B 细胞淋巴瘤患者分期和治疗中的作用。流式细胞术鉴定出仅占脑脊液淋巴细胞总数 0.02% 的肿瘤克隆。流式细胞术检测到细胞学、化学和细胞计数失败的情况。流式细胞术检测疾病是一个负面的预后因素，现在提示治疗干预。该研究的结果是，建议有侵袭性 B 细胞淋巴瘤累及 CNS 风险的患者通过流式细胞术进行 CSF 分期评估。流式细胞术单位参加了国际共识会议，以确定脑脊液流式细胞术分析的最佳方法。