Flow cytometric detection of malignant cells in body fluids

流式细胞仪检测体液中的恶性细胞

• 批准号: 10926619
• 负责人: Hao-Wei Wang
• 金额: $ 28.57万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10926619
• 关键词: B-Cell Lymphomas; Biological Assay; Body Fluids; Cell Count; Cerebrospinal Fluid; Chemistry; Clinical; Consensus; Cytology; Detection; Diagnosis; Disease; Evaluation; Flow Cytometry; Goals; HIV; Hematopoietic Neoplasms; Human Herpesvirus 8; International; Liquid substance; Lymphocyte; Lymphoma; Methodology; Molecular Diagnosis; Morphology; Multicentric Angiofollicular Lymphoid Hyperplasia; Neoplasms; Patients; Physical shape; Pleural; Process; Prognostic Factor; Protocols documentation; Rare Diseases; Recommendation; Risk; Role; Series; Staging; Techniques; Therapeutic; Therapeutic Intervention; Vitreous humor; cancer cell; improved; leukemia; neoplastic; primary effusion lymphoma; prognostic; symposium

Primary effusion lymphoma is an aggressive lymphoma arising in the setting of HIV and HHV8 that primarily involves body fluids. Multicentric Castlemans disease is a non-clonal proliferative process that arises in the same clinical setting. Morphological diagnosis is difficult in these rare diseases. We have developed clinical flow cytometric assays that can aid in the diagnosis of primary effusion lymphoma and multicentric Castlemans disease, providing key clinical support to patients on NCI protocols. Involvement of the cerebrospinal fluid (CSF) by hematopoietic malignancies may also be difficult to document by morphology alone. In a study comparing morphology alone to morphology with flow cytometry, we demonstrated that flow cytometric immunophneotyping was useful in establishing a diagnosis of neoplasia in a series of patients with known lymphoma or leukemia and an initial diagnosis of "atypical" or "suspicious" CSF using morphologic criteria. The Flow Cytometry Unit evaluated the role of flow cytometric analysis in staging and management of patients with high grade B cell lymphomas. Flow cytometry identified neoplastic clones that constituted as little as 0.02% of total CSF lymphocytes. Flow cytometry detected involvement where cytology, chemistry and cell counts failed. Flow cytometric detection of disease was a negative prognostic factor and now prompts therapeutic intervention. As a result of the study it is recommended that patients at risk for CNS involvement by aggressive B cell lymphoma undergo staging CSF evaluation by flow cytometry. The Flow Cytometry Unit participated in an international consensus conference to determine optimal methodology for flow cytometric analysis of CSF.

原发性渗出性淋巴瘤是一种在HIV和HHV 8背景下产生的侵袭性淋巴瘤，主要涉及体液。多中心Castleman病是一种非克隆性增殖过程，出现在相同的临床环境。这些罕见疾病的形态学诊断是困难的。我们开发了临床流式细胞术检测方法，可以帮助诊断原发性渗出性淋巴瘤和多中心Castleman病，为NCI方案的患者提供关键的临床支持。造血系统恶性肿瘤累及脑脊液（CSF）也可能难以单独通过形态学记录。在一项比较单纯形态学与流式细胞术形态学的研究中，我们证明了流式细胞术免疫分型在一系列已知淋巴瘤或白血病患者中建立肿瘤诊断以及使用形态学标准初步诊断“非典型”或“可疑”CSF中是有用的。流式细胞术室评估了流式细胞术分析在高级别B细胞淋巴瘤患者分期和管理中的作用。流式细胞术鉴定了占总CSF淋巴细胞的0.02%的肿瘤克隆。流式细胞术检测参与细胞学，化学和细胞计数失败。流式细胞术检测疾病是一个消极的预后因素，现在提示治疗干预。作为研究的结果，建议有侵袭性B细胞淋巴瘤累及CNS风险的患者接受流式细胞术分期CSF评价。流式细胞术单位参加了一次国际共识会议，以确定CSF流式细胞术分析的最佳方法。

项目成果

Flow cytometry increases the sensitivity of detection of leukemia and lymphoma cells in bronchoalveolar lavage specimens.

流式细胞术提高了支气管肺泡灌洗标本中白血病和淋巴瘤细胞检测的灵敏度。

• DOI: 10.1002/cyto.b.21033
• 发表时间: 2012
• 期刊: Cytometry. Part B, Clinical cytometry
• 作者: Song,JooY;Filie,ArmandoC;Venzon,David;Stetler-Stevenson,Maryalice;Yuan,ConstanceM
• 通讯作者: Yuan,ConstanceM

Detection and outcome of occult leptomeningeal disease in diffuse large B-cell lymphoma and Burkitt lymphoma.

弥漫性大 B 细胞淋巴瘤和伯基特淋巴瘤中隐匿性软脑膜疾病的检测和结果。

• DOI: 10.3324/haematol.2013.101741
• 发表时间: 2014
• 期刊: Haematologica
• 影响因子: 10.1
• 作者: Wilson,WyndhamH;Bromberg,JacolineEC;Stetler-Stevenson,Maryalice;Steinberg,SethM;Martin-Martin,Lourdes;Muñiz,Carmen;Sancho,JuanManuel;Caballero,MariaDolores;Davidis,MarjanA;Brooimans,RikA;Sanchez-Gonzalez,Blanca;Salar,Antonio
• 通讯作者: Salar,Antonio

Complete Remissions of Adult T-cell Leukemia with Anti-CD25 Recombinant Immunotoxin LMB-2 and Chemotherapy to Block Immunogenicity.

• DOI: 10.1158/1078-0432.ccr-15-1412
• 发表时间: 2016-01-15
• 期刊: Clinical cancer research : an official journal of the American Association for Cancer Research
• 作者: Kreitman RJ;Stetler-Stevenson M;Jaffe ES;Conlon KC;Steinberg SM;Wilson W;Waldmann TA;Pastan I
• 通讯作者: Pastan I

Persistent non-neoplastic gammadelta-T cells in cerebrospinal fluid of a patient with hepatosplenic (gammadelta) T cell lymphoma: a case report with 6 years of flow cytometry follow-up.

肝脾（γδ）T 细胞淋巴瘤患者脑脊液中持续存在非肿瘤性γδ-T 细胞：经过 6 年流式细胞术随访的病例报告。

• 发表时间: 2009
• 期刊: International journal of clinical and experimental pathology
• 影响因子: 1.4
• 作者: Jiang,Liuyan;Abati,AndreaD;Wilson,Wyndham;Stetler-Stevenson,Maryalice;Yuan,Constance
• 通讯作者: Yuan,Constance

25-Gauge transconjunctival sutureless vitrectomy for the diagnosis of intraocular lymphoma.

• DOI: 10.1136/bjo.2009.167940
• 发表时间: 2010-05
• 期刊: The British journal of ophthalmology
• 作者: Yeh S;Weichel ED;Faia LJ;Albini TA;Wroblewski KK;Stetler-Stevenson M;Ruiz P;Sen HN;Chan CC;Nussenblatt RB
• 通讯作者: Nussenblatt RB