Establishing a clinical research flow cytometry core

建立临床研究流式细胞术核心

• 批准号: 10703088
• 负责人: Kaska Wloka
• 金额: $ 36.9万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10703088
• 关键词: Adult; Biological Assay; Cancer Patient; Cell Therapy; Cells; Childhood; Clinical; Clinical Investigator; Clinical Research; Clinical Trials; Collaborations; Data; Data Analyses; Development; Disease Progression; Equipment; Evaluation; Experimental Designs; Flow Cytometry; Goals; Human Resources; Immune; Immune system; Immunologic Deficiency Syndromes; Immunology; Immunotherapy; Infusion procedures; Knowledge; Laboratories; Lymphocyte; Malignant Neoplasms; Malignant lymphoid neoplasm; Mission; Modernization; Multiple Myeloma; NCI Center for Cancer Research; National Cancer Institute; Pediatric Oncology; Phenotype; Plasma Cells; Preparation; Procedures; Process; Reagent; Research Personnel; Resources; Sampling; Services; Technology; Therapeutic Effect; Training; United States National Institutes of Health; Wiskott-Aldrich Syndrome; anticancer research; bioinformatics tool; chimeric antigen receptor T cells; chronic graft versus host disease; design; experience; fighting; high dimensionality; improved; interest; novel; novel therapeutics; pediatric patients; programs; skills; therapeutically effective; young adult

The Clinical Research Flow Cytometry core is currently in the final stage of the set up process having just acquired additional equipment, space and personnel as well as all required laboratory consumables and reagents. Space remodeling has been completed and data analysis workstation established. Advice and extensive experience of other expert NIH flow cytometrists, including both clinical and research core managers, have been appreciated during the design stage (Maryalice Stetler-Stevenson, Hao-Wei Wang, Constance Yuan, Pradeep Dagur, Karen Laky, Katherine McKinnon, Iyadh Douagi, Ferenc Livak). The core has been staffed with experts in both flow cytometry and immunology and as such will provide a unique set of skills and knowledge to clinical investigators. The facility has just started to provide support to investigators in 1) Pediatric Oncology Branch, studying the immunophenotypic changes that CAR T cells undergo after infusion into pediatric and young adult cancer patients (Nirali Shah, Naomi Taylor); 2) Lymphoid Malignancy Branch, defining the effect of immunotherapy on the phenotype and function of abnormal plasma cells in multiple myeloma (Elizabeth Hill); 3) Immune Deficiency Cellular Therapy Program in evaluating lymphocytic phenotype and function in pediatric patients with immunodeficiencies such as Wiskott-Aldrich syndrome and DOCK8 deficiency (Sung-Yun Pai) and in adults with chronic GvHD treated with novel therapies (Steven Pavletic). The core contributes to the development of the bioinformatic tools for flow cytometric panel design via the newly-established collaboration with the group of Gregoire Altan-Bonnett.

临床研究流式细胞术核心目前处于建立过程的最后阶段，刚刚获得了额外的设备、空间和人员以及所有必要的实验室耗材和试剂。完成空间改造，建立数据分析工作站。在设计阶段，NIH流式细胞仪专家（包括临床和研究核心管理人员）的建议和丰富经验得到了赞赏（Maryalice steler - stevenson, Hao-Wei Wang, Constance Yuan, Pradeep Dagur, Karen Laky, Katherine McKinnon, Iyadh Douagi, Ferenc Livak）。该中心配备了流式细胞术和免疫学专家，因此将为临床研究人员提供一套独特的技能和知识。该设施刚刚开始为以下研究人员提供支持：1)儿科肿瘤科，研究CAR - T细胞输注到儿科和年轻成人癌症患者体内后的免疫表型变化（Nirali Shah, Naomi Taylor）；2)淋巴恶性肿瘤分支，定义免疫治疗对多发性骨髓瘤异常浆细胞表型和功能的影响（Elizabeth Hill）；3)免疫缺陷细胞治疗项目，评估儿童免疫缺陷患者（如Wiskott-Aldrich综合征和DOCK8缺乏症（Sung-Yun Pai））和成人慢性GvHD患者（Steven Pavletic）的淋巴细胞表型和功能。通过与Gregoire Altan-Bonnett小组的新建立的合作，核心有助于流式细胞仪面板设计的生物信息学工具的发展。