Clinical Research Correlatives Core

临床研究相关核心

• 批准号: 10926665
• 负责人: Kaska Wloka
• 金额: $ 134.51万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10926665
• 关键词: Adult; Allogenic; Autologous; Biological Assay; Cancer Patient; Cell Therapy; Cells; Childhood; Clinical; Clinical Investigator; Clinical Protocols; Clinical Research; Clinical Trials; Collaborations; Custom; Data; Development; Disease Progression; Evaluation; Experimental Designs; Flow Cytometry; Goals; HIV/AIDS; Human Herpesvirus 8; Immune; Immune system; Immunologic Deficiency Syndromes; Immunology; Immunooncology; Immunophenotyping; Immunotherapy; Infusion procedures; Interleukin-15; Knowledge; Laboratories; Lymphocyte; Lymphoma; Malignant Neoplasms; Malignant lymphoid neoplasm; Mission; Modernization; Multicentric Angiofollicular Lymphoid Hyperplasia; Multiple Myeloma; NCI Center for Cancer Research; National Cancer Institute; Natural Killer Cells; Pediatric Oncology; Phenotype; Plasma Cells; Pleural effusion disorder; Preparation; Procedures; Research; Research Personnel; Resources; Sampling; Services; Stains; Stem cell transplant; Technology; Therapeutic Effect; Training; United States National Institutes of Health; Wiskott-Aldrich Syndrome; anticancer research; bioinformatics tool; cancer therapy; chimeric antigen receptor T cells; chronic graft versus host disease; design; experience; fighting; high dimensionality; immune reconstitution; improved; interest; keratinocyte growth factor; novel; novel therapeutics; pediatric patients; programs; recruit; skills; therapeutically effective; young adult

The Clinical Research Correlatives Core (CRCC) - a state-of-the-art clinical research flow cytometry core employing spectral technology for high dimensional multi-parameter cell analysis - has been fully established and opened to the CCR's investigators. The design stage has benefited from the advice and extensive experience of other expert NIH flowcytometrists, including both clinical and research core managers (Maryalice Stetler-Stevenson, Hao-Wei Wang, Constance Yuan, Pradeep Dagur, Karen Laky, Katherine McKinnon, Iyadh Douagi, Ferenc Livak). The core has been staffed with experts in both flow cytometry and immunology, and as such, provides a unique set of skills and knowledge to clinical investigators. Additional technical staff were recently recruited and fully trained to meet growing demand for the core's support. The facility offers extended service to include experimental design, assay optimization, sample processing (including staining), acquisition and analysis. Using either custom-designed assays or some of the 8 already optimized (CYTEK) panels, CRCC supports CCR's investigators in the: 1) Center for Immuno-Oncology, evaluating the effect of autologous and allogeneic stem cell transplant on immune reconstitution (Christopher Kanakry, Jennifer Kanakry and Dimana Dimitrova) 2) Pediatric Oncology Branch, studying the immunophenotypic changes that CAR T cells (or autologous IL-15 activated NK cells) undergo after infusion into pediatric and young adult cancer patients (Nirali Shah, Haneen Shalabi, Rosie Kaplan, Rosa Nguyen). These studies aim at improving adoptive cancer therapies. 3) HIV and AIDS Malignancy Branch, characterizing the phenotype of Kaposi Sarcoma Herpes Virus- associated hematolymphoid malignancies such as Multicentric Castleman's Disease and Pleural Effusion Lymphoma (Ramya Ramaswami and Laurie Krug) 4) Lymphoid Malignancy Branch, defining the effect of immunotherapy on the phenotype and function of abnormal plasma cells in multiple myeloma (Elizabeth Hill) 5) Immune Deficiency Cellular Therapy Program, evaluating lymphocytic phenotype and function in pediatric patients with immunodeficiencies such as Wiskott-Aldrich syndrome and DOCK8 deficiency (Sung-Yun Pai) and in adults with chronic GvHD treated with novel therapies such as palifermin (Steven Pavletic). Overall, the facility has been involved in supporting 18 clinical protocols with either already established assays or those currently being optimized (custom). The facility also contributes to the development of the bioinformatic tools for flowcytometric panel design through collaboration with the group of Gregoire Altan-Bonnett.

临床研究相关核心（CRCC）--采用光谱技术进行高维多参数细胞分析的最先进的临床研究流式细胞术核心--已经完全建立并向CCR的研究者开放。设计阶段得益于其他NIH流式细胞仪专家的建议和丰富经验，包括临床和研究核心经理（Maryalice Stetler-Stevenson、Hao-Wei Wang、Constance Yuan、Pradeep Dagur、Karen Laky、凯瑟琳麦金农、Iyadh Douagi、Ferenc Livak）。该中心配备了流式细胞术和免疫学方面的专家，因此为临床研究者提供了一套独特的技能和知识。最近征聘了更多的技术工作人员并对其进行了充分培训，以满足对核心支助的日益增长的需求。该设施提供扩展服务，包括实验设计，分析优化，样品处理（包括染色），采集和分析。通过使用定制设计的检测试剂盒或8种已优化的（CYTEK）检测试剂盒中的一些，CRCC在以下方面为CCR的研究人员提供支持：1）免疫肿瘤中心，评估自体和异体干细胞移植对免疫重建的影响（克里斯托弗·卡纳克里，詹妮弗·卡纳克里和迪马纳·迪米特洛娃）2）儿科肿瘤学分支，研究CAR T细胞（或自体IL-15激活的NK细胞）在输注到儿科和年轻成人癌症患者中后发生的免疫表型变化（Nirali Shah，Haneen Shalabi，Rosie Kaplan，Rosa Nguyen）。这些研究旨在改善过继性癌症治疗。3)艾滋病毒和艾滋病分支分支，以卡波西肉瘤疱疹病毒相关血液淋巴样恶性肿瘤（如多中心Castleman病和胸膜渗出性淋巴瘤）的表型为特征（Ramya Ramaswami和劳里克鲁格）4）类风湿性关节炎分支，定义免疫治疗对多发性骨髓瘤异常浆细胞表型和功能的影响（伊丽莎白希尔）5）免疫缺陷细胞治疗计划，评估免疫缺陷（如Wiskott-Aldrich综合征和DOCK 8缺陷）儿童患者的淋巴细胞表型和功能（Sung-Yun派）和用新疗法如帕利弗明（Steven Pavletic）治疗的患有慢性GvHD的成年人中。总体而言，该设施已参与支持18个临床方案，其中包括已建立的测定或目前正在优化的测定（定制）。该设施还通过与Gregoire Altan-Bonnett小组的合作，为流式细胞仪面板设计的生物信息学工具的开发做出了贡献。