Affordable sialoglycans and associated reagents for expanded chemoenzymatic production
用于扩大化学酶生产的经济实惠的唾液酸聚糖和相关试剂
基本信息
- 批准号:10693333
- 负责人:
- 金额:$ 65.34万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-09-15 至 2025-08-31
- 项目状态:未结题
- 来源:
- 关键词:AcidsAminesAnimalsBacterial InfectionsBasic ScienceBinding ProteinsBiologicalBiotinylationCarbonCell surfaceChemicalsCollaborationsCommunitiesComplexCytolysisDiagnosticDisaccharidesDiseaseEnzymesEquilibriumEventFamilyFamily memberFermentationFutureGlycolipidsGlycoproteinsGoalsHomeostasisHomologous GeneHumanHuman BiologyHuman MilkHuman PathologyHuman bodyHydrophobicityIn SituIndustryInfrastructureMalignant NeoplasmsMammalsMethodsMolecular ProbesMonitorMonosaccharidesN-Acetylneuraminic AcidNeoplasm MetastasisOligosaccharidesPathogenesisPathologic ProcessesPhysiological ProcessesPlayPolysaccharidesPriceProcessProductionProductivityPropertyProtein EngineeringProtocols documentationReactionReaction TimeReagentResearchRoleSalesSialic AcidsSystemTechniquesTestingTherapeuticVertebral columnVirus Diseasescandidate identificationcommercializationcommercialization readinesscostglycosylationglycosyltransferasehuman diseaseimmunoregulationimprovedinnovationlarge scale productionmannosaminemanufacturemutantresearch and developmentsugarsugar nucleotidetherapeutic development
项目摘要
Project Summary
Sialoglycans are sialic acid-containing oligosaccharides that play important biological roles in human biology and
pathology and are indispensable molecular probes for research related to bacterial and viral infection, cancer
metastasis, immune regulation, etc. The multifaceted functions of sialoglycans in human milk are also being
explored. Nevertheless, sialoglycan-related research and development of therapeutics and diagnostics have
been hampered by the limited access to these structurally complex compounds as well as the high cost and
special expertise needed for synthesizing and purifying these reagents.
Among various synthetic strategies for producing sialoglycans, glycosyltransferase-based chemoenzymatic
methods are attractive strategies. An efficient method for synthesizing structurally defined sialoglycans is one-
pot multienzyme (OPME) chemoenzymatic strategy developed by Dr. Hai Yu and Dr. Xi Chen at UC Davis. Each
OPME glycosylation reaction contains a glycosyltransferase and enzymes involved in generating the
corresponding sugar nucleotide donor in situ from a simple monosaccharide. The OPME reactions allow the
synthesis of target glycans containing the desired glycosidic linkage with high regio- and stereo-specificity. At
the current stage, large-scale synthesis by the OPME systems is still limited by the scales and the yields of the
enzymes produced. In addition, OPME reaction conditions can be improved to decrease the amounts of enzymes
used for the synthesis. Product purification processes can also be streamlined using innovative strategies.
To advance sialic acid-related research and therapeutic development, the goal of this proposed project is to
develop reagents, enzymes, and methods that are ready for commercialization to allow low-cost access to
sialoglycans of high demands and their underlying asialoglycans by the broad scientific community (both
academic and industry) and to enable highly efficient production of diverse biologically important special
sialoglycan targets by even nonspecialists. This will be achieved by the collaboration of the UC Davis team with
IMCS which has expertise in high-yield enzyme production by fermentation, unique lysis technique, commercial
infrastructure to manufacture, and product sale at affordable prices to the community.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Lim Andrew Lee其他文献
Lim Andrew Lee的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Lim Andrew Lee', 18)}}的其他基金
Affordable sialoglycans and associated reagents for expanded chemoenzymatic production
用于扩大化学酶生产的经济实惠的唾液酸聚糖和相关试剂
- 批准号:
10324760 - 财政年份:2021
- 资助金额:
$ 65.34万 - 项目类别:
Affordable sialoglycans and associated reagents for expanded chemoenzymatic production
用于扩大化学酶生产的经济实惠的唾液酸聚糖和相关试剂
- 批准号:
10582767 - 财政年份:2021
- 资助金额:
$ 65.34万 - 项目类别:
Developing chemoenzymatic strategies, enzymes, and kits for accessible and affordable gangliosides
开发化学酶策略、酶和试剂盒,以获得可获取且负担得起的神经节苷脂
- 批准号:
10267384 - 财政年份:2020
- 资助金额:
$ 65.34万 - 项目类别:
Developing chemoenzymatic strategies, enzymes, and kits for accessible and affordable gangliosides
开发化学酶策略、酶和试剂盒,以获得可获取且负担得起的神经节苷脂
- 批准号:
10482382 - 财政年份:2020
- 资助金额:
$ 65.34万 - 项目类别:
Developing chemoenzymatic strategies, enzymes, and kits for accessible and affordable gangliosides
开发化学酶策略、酶和试剂盒,以获得可获取且负担得起的神经节苷脂
- 批准号:
10080549 - 财政年份:2020
- 资助金额:
$ 65.34万 - 项目类别:
Developing chemoenzymatic strategies, enzymes, and kits for accessible and affordable gangliosides
开发化学酶策略、酶和试剂盒,以获得可获取且负担得起的神经节苷脂
- 批准号:
10584172 - 财政年份:2020
- 资助金额:
$ 65.34万 - 项目类别:
相似海外基金
More sustainable biocatalytic imine reductions to chiral amines with hydrogen-driven NADPH recycling operated in batch and continuous flow
通过批量和连续流操作的氢驱动 NADPH 回收,更可持续地生物催化亚胺还原为手性胺
- 批准号:
2889869 - 财政年份:2023
- 资助金额:
$ 65.34万 - 项目类别:
Studentship
Organoborane-catalysed approaches to biologically active amines
有机硼烷催化制备生物活性胺的方法
- 批准号:
EP/Y00146X/1 - 财政年份:2023
- 资助金额:
$ 65.34万 - 项目类别:
Research Grant
Transforming Amines into Complex Polycyclic Molecules and Bioactive Natural Products
将胺转化为复杂的多环分子和生物活性天然产物
- 批准号:
2247651 - 财政年份:2023
- 资助金额:
$ 65.34万 - 项目类别:
Standard Grant
Ti-catalyzed cascading hydroaminoalkylation as a route to complex functionalized amines
Ti 催化级联氢氨基烷基化作为制备复杂官能化胺的途径
- 批准号:
10750347 - 财政年份:2023
- 资助金额:
$ 65.34万 - 项目类别:
New Photocatalytic C-C Bond-Forming Reactivity of Unprotected Primary Amines
未受保护伯胺的新光催化 C-C 键形成反应
- 批准号:
EP/X026566/1 - 财政年份:2023
- 资助金额:
$ 65.34万 - 项目类别:
Research Grant
Nickel Cross-Coupling Cascades with α-Heteroatom Radicals to Prepare Sterically Hindered Alcohols and Amines
镍与α-杂原子自由基交叉偶联级联制备位阻醇和胺
- 批准号:
10604535 - 财政年份:2023
- 资助金额:
$ 65.34万 - 项目类别:
Towards a better understanding of the effect of the pentafluorosulfanyl group on the lipophilicity and acid/base properties of alcohols and amines
更好地了解五氟硫基对醇和胺的亲脂性和酸/碱性质的影响
- 批准号:
571856-2021 - 财政年份:2022
- 资助金额:
$ 65.34万 - 项目类别:
Alliance Grants
Development of Strategies for the Enantioselective Synthesis of Heterocycles and Acyclic Amines
杂环和无环胺对映选择性合成策略的发展
- 批准号:
10656344 - 财政年份:2022
- 资助金额:
$ 65.34万 - 项目类别:
Pd-Catalyzed C(sp3)-H Functionalizations Directed by Free Alcohols and Boc-Protected Amines
由游离醇和 Boc 保护的胺引导的 Pd 催化 C(sp3)-H 官能化
- 批准号:
10606508 - 财政年份:2022
- 资助金额:
$ 65.34万 - 项目类别:














{{item.name}}会员




