Revealing protein-protein interactions and RNA-protein interactions at genome-scale in two weeks

两周内揭示基因组规模的蛋白质-蛋白质相互作用和 RNA-蛋白质相互作用

基本信息

  • 批准号:
    10693917
  • 负责人:
  • 金额:
    $ 37.7万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2020
  • 资助国家:
    美国
  • 起止时间:
    2020-09-18 至 2024-08-31
  • 项目状态:
    已结题

项目摘要

Revealing protein-protein interactions and RNA-protein interactions at genome-scale in two weeks Abstract Our ability to interpret the human genome is limited by our knowledge of the interaction networks of the products of the genome sequence, including RNAs and proteins. If we had a complete reference map of all DNA-DNA, protein-DNA, RNA-DNA, RNA-RNA, RNA-protein, and protein-protein interactions, we would have a completely new approach to reading the book of the human genome. Recent technology breakthroughs, including those led by NIH Common Fund programs, enabled genome-wide mapping of DNA-DNA, protein-DNA, RNA-DNA, and RNA-RNA interactions en masse. However, genome-scale mapping of RNA-protein interactions (RPI) and protein-protein interactions (PPI) remain laborious and resource-intensive. In this project, we propose two extremely high-throughput genomic-based technologies for mapping human RPI and PPI networks at genome scale. We will develop bioinformatics tools to analyze the data with statistical rigor. Specifically, we propose an “all-vs-all” approach to map PPI and RPI networks. These genomics technologies and their coupled genomic informatics tools will generate reference maps of human PPI and RPI networks. In the long term, such maps will greatly facilitate the interpretation of the functions of the human genome. In Aim 1, we will develop an extremely high-throughput technology called “PPI-seq” to map the PPI network at genome-scale. PPI-seq is expected to be capable of generating a reference map of the human PPI network within a single lab in 2 weeks' time, with an expected yield of ~50,000 high-confidence pairwise PPIs. In Aim 2, we will develop an extremely high- throughput technology called “RPI-seq” to map the RPI network at genome-scale. RPI-seq is expected to be capable of generating a reference map of the human RPI network within single lab in 2 weeks' time. RPI-seq will simultaneous reveal RNA binding proteins (RBP) and the RNAs bound by each RBP.
在两周内揭示基因组尺度下的蛋白质相互作用和rna -蛋白质相互作用

项目成果

期刊论文数量(0)
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会议论文数量(0)
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Sheng Zhong其他文献

Sheng Zhong的其他文献

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{{ truncateString('Sheng Zhong', 18)}}的其他基金

Revealing protein-protein interactions and RNA-protein interactions at genome-scale in two weeks
两周内揭示基因组规模的蛋白质-蛋白质相互作用和 RNA-蛋白质相互作用
  • 批准号:
    10470199
  • 财政年份:
    2020
  • 资助金额:
    $ 37.7万
  • 项目类别:
Extremely high-throughput mapping of protein, RNA, and chromatin interactions in health and disease
健康和疾病中蛋白质、RNA 和染色质相互作用的极高通量图谱
  • 批准号:
    10226341
  • 财政年份:
    2020
  • 资助金额:
    $ 37.7万
  • 项目类别:
Spatial in situ mapping of RNA-chromatin interactions at transcriptome-and-genome scale in human tissues
人体组织转录组和基因组尺度 RNA-染色质相互作用的空间原位作图
  • 批准号:
    10876922
  • 财政年份:
    2020
  • 资助金额:
    $ 37.7万
  • 项目类别:
Spatial in situ mapping of RNA-chromatin interactions at transcriptome-and-genome scale in human tissues
人体组织转录组和基因组尺度 RNA-染色质相互作用的空间原位作图
  • 批准号:
    10119123
  • 财政年份:
    2020
  • 资助金额:
    $ 37.7万
  • 项目类别:
Revealing protein-protein interactions and RNA-protein interactions at genome-scale in two weeks
两周内揭示基因组规模的蛋白质-蛋白质相互作用和 RNA-蛋白质相互作用
  • 批准号:
    10032303
  • 财政年份:
    2020
  • 资助金额:
    $ 37.7万
  • 项目类别:
Revealing protein-protein interactions and RNA-protein interactions at genome-scale in two weeks
两周内揭示基因组规模的蛋白质-蛋白质相互作用和 RNA-蛋白质相互作用
  • 批准号:
    10265495
  • 财政年份:
    2020
  • 资助金额:
    $ 37.7万
  • 项目类别:
Spatial in situ mapping of RNA-chromatin interactions at transcriptome-and-genome scale in human tissues
人体组织转录组和基因组尺度 RNA-染色质相互作用的空间原位作图
  • 批准号:
    10687345
  • 财政年份:
    2020
  • 资助金额:
    $ 37.7万
  • 项目类别:
Extremely high-throughput mapping of protein, RNA, and chromatin interactions in health and disease
健康和疾病中蛋白质、RNA 和染色质相互作用的极高通量图谱
  • 批准号:
    10453756
  • 财政年份:
    2020
  • 资助金额:
    $ 37.7万
  • 项目类别:
Extremely high-throughput mapping of protein, RNA, and chromatin interactions in health and disease
健康和疾病中蛋白质、RNA 和染色质相互作用的极高通量图谱
  • 批准号:
    10653995
  • 财政年份:
    2020
  • 资助金额:
    $ 37.7万
  • 项目类别:
Extremely high-throughput mapping of protein, RNA, and chromatin interactions in health and disease
健康和疾病中蛋白质、RNA 和染色质相互作用的极高通量图谱
  • 批准号:
    10062757
  • 财政年份:
    2020
  • 资助金额:
    $ 37.7万
  • 项目类别:

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