Development of Degradable Fibers with Hemostatic Properties

具有止血性能的可降解纤维的开发

• 批准号: 10707477
• 负责人: Sharon K Hamilton
• 金额: $ 15.52万
• 依托单位: OUACHITA BAPTIST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-20 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10707477
• 关键词: 3T3 Cells; Address; Amides; Architecture; Biocompatible Materials; Biological Assay; Biomimetics; Biopolymers; Cell Culture Techniques; Chitosan; Coagulation Process; Collagen; Complement; Complex; Coupled; Coupling; Debridement; Development; Electrospinning; Environment; Enzymes; Evaluation; Evolution; Excision; Exhibits; Fiber; Future; Goals; Hemorrhage; Hemostatic Agents; Hemostatic function; Histologic; Image; In Vitro; Internet; Knowledge; Laboratories; Modeling; Modernization; Mus; NIH 3T3 Cells; Nanofiber Scaffold; Organic Chemistry; Patients; Physiological; Polyesters; Polymers; Property; Publishing; Reaction; Research; Second Look Surgery; Students; Testing; Thrombin; Time; Tissues; Work; Wound models; analog; biomaterial compatibility; caprolactone; career; cost; emergency settings; experience; functional group; graduate school; in vivo; in vivo evaluation; innovation; light weight; liver injury; macromolecule; monomer; nanofiber; novel; polycaprolactone; prevent; regenerative; regenerative treatment; response; scaffold; skills; subcutaneous; symposium; tissue regeneration; undergraduate student; wound dressing; wound healing

PROJECT SUMMARY Recent evolutions in the field of hemostatic materials have focused on developing products with high hemostatic efficiency. An ideal hemostatic material should quickly control hemorrhaging, exhibit biocompatibility and degradability, promote wound healing and be lightweight and inexpensive. Electrospinning degradable macromolecules like collagen, chitosan, polycaprolactone yields nanofiber which have shown promise in wound healing and bleeding cessation applications. Modern bleeding cessation treatments should have degradable and regenerative capabilities but little research has been published on the utilization of synthetic analogs to costly biomolecules. The long-term goal of this project is to generate a better understanding of polymer-based hemostatic wound healing materials and cellular responses towards these materials for implementation in regenerative treatments. As a step towards this goal, a degradable collagen strand analog must be synthesized, electrospun, and compared to other scaffolds for in vitro and in vivo evaluation. It is hypothesized that a biomimetic synthetic polymer can be electrospun into an affordable, biocompatible, degradable, hemostatic wound dressing material that will not only catalyze coagulation but also will not require surgical removal. This proposal is significant because it represents the first attempt to incorporate a polyester-based collagen mimic into electrospun scaffolds with the ability to control scaffold composition to combine desired properties and functionalities in an ideal material for wound healing and hemostatic applications. This approach is innovative because it will determine the feasibility of utilizing a synthetic collagen mimic in applications such as electrospun wound healing and bleeding cessation materials. It is anticipated that the collagen-like functional groups on the novel polyester coupled with the presence of chitosan in the fibers will lead to bleeding cessation in wound models with suitable degradation rates. Furthermore, undergraduate students will conduct the majority of the research in this proposal. This will be a significant research experience for these students and will provide them with valuable laboratory and research skills that will be essential in their careers.

项目概要 止血材料领域的最新发展重点是开发具有高止血性能的产品 效率。理想的止血材料应能快速控制出血、具有生物相容性和 可降解性、促进伤口愈合、重量轻、价格便宜。静电纺丝可降解 胶原蛋白、壳聚糖、聚己内酯等大分子产生的纳米纤维在伤口方面显示出应用前景 愈合和止血应用。现代止血治疗应该具有可降解和 再生能力，但关于利用合成类似物来实现昂贵的再生能力的研究很少 生物分子。该项目的长期目标是更好地了解基于聚合物的 止血伤口愈合材料和细胞对这些材料的反应，用于实施 再生治疗。 作为实现这一目标的一步，必须合成、静电纺丝和可降解的胶原链类似物 与其他支架进行体外和体内评估比较。据推测，仿生合成 聚合物可以静电纺丝成一种经济实惠、生物相容、可降解、止血的伤口敷料材料 这不仅会催化凝血，而且不需要手术切除。 该提案意义重大，因为它代表了将聚酯基材料纳入其中的首次尝试 胶原模拟物进入电纺支架，能够控制支架成分以结合所需的 用于伤口愈合和止血应用的理想材料的特性和功能。这种做法 具有创新性，因为它将确定在诸如此类的应用中使用合成胶原模拟物的可行性 作为静电纺丝伤口愈合和止血材料。预计胶原蛋白样 新型聚酯上的官能团与纤维中壳聚糖的存在相结合将导致 具有适当降解率的伤口模型中的出血停止。此外，本科生将 进行本提案中的大部分研究。对于这些人来说，这将是一次重要的研究经历 学生，将为他们提供宝贵的实验室和研究技能，这对于他们的 职业生涯。