Stem Cell Integral Membrane Transporter ABCB5 and Dermal Regeneration

项目总结 项目3将测试以下中心假设：(1)ABCB5()真皮间充质干细胞(DMSc) 正常成人皮肤动态平衡和再生所需；(2)ABCB5()DMSc下降是相关因素 用于老化过程中受损的皮肤动态平衡和再生伤口愈合；以及(3)ABCB5()DMSc 移植具有修复与年龄相关的皮肤动态平衡受损的治疗潜力 再生。这项研究的总体意义是研究范式的潜在转变 从这项研究的成功完成：(1)拟议的实验将回答一个基本问题 干细胞生物学和衰老中尚未在前瞻性研究中解决的问题 成人干细胞室中选择性功能障碍的程度如何(相对于 分化的组织)对组织衰老负责？以及(2)再生组织的记录 ABCB5()DMSc的能力及其作为年龄相关性损害的原因的证据 皮肤再生能力的提高将为这一候选细胞的定向应用奠定基础 用于治疗老年患者的皮肤伤口。

PROJECT SUMMARY Project 3 will test the central hypotheses that (1) ABCB5(+) dermal mesenchymal stem cells (DMSC) are required for normal adult skin homeostasis and regeneration; (2) ABCB5(+) DMSC decline is responsible for impaired skin homeostasis and regenerative wound healing during aging; and (3) ABCB5(+) DMSC transplantation possesses therapeutic potential to restore age-related impairment of skin homeostasis and regeneration. The overall significance of this study are the potential shifts in research paradigms resulting from successful completion of this research: (1) The proposed experiments will answer a fundamental question in stem cell biology and aging that has not been addressed as-of-yet in prospective studies – To what extent is selective dysfunction in an adult stem cell compartment (vis-à-vis dysregulation of differentiated tissues) responsible for organismal aging?; and (2) Documentation of the regenerative capacity of ABCB5(+) DMSC along with demonstration of their decline as a cause age-related impairment of skin regenerative capacity will lay the foundation for targeted employment of this candidate cell therapeutic for the treatment of cutaneous wounds in aging patients.