Microbiome profiles, sleep, and cognition among mid-life Latinx adults

FIU-RCMI研究项目#2 项目摘要 新出现的证据表明，睡眠障碍和肠道改变之间存在双向关系 微生物组。这项研究旨在考察睡眠健康和睡眠障碍对认知的影响。 同时解释了肠道微生物群在基于社区的Latinx样本中的作用。尽管它的 重要的是，研究探索睡眠对肠道微生物群的影响，以及随后对 人类的认知是极其有限的。在确实存在的初步调查中，没有一项是 使用拉丁裔社区生活样本进行了调查。而Latinx样本是两个肠道微生物组的焦点 研究为这一群体独特的肠道微生物群提供了初步证据，我们的 建议的研究具有创新性，因为它包括认知测量、皮质醇采样和客观测量 睡着了。在国家少数民族健康和健康差距框架的指导下，我们还将 探索各种社会文化因素对睡眠、认知和肠道的影响程度 微生物组。鉴于肠道微生物群可能具有直接的 对认知和睡眠的影响，将其定位为认知下降的可改变的风险因素。睡眠障碍， 包括阿尔茨海默病在内的认知衰退的一个可改变的风险因素与肠道双向相关 微生物群，睡眠模式的变化影响肠道微生物群的结构和多样性，以及肠道 微生物群对昼夜节律和与睡眠和清醒相关的荷尔蒙有影响。调查 在以社区为基础的样本中，检查微生物区系-肠道-脑轴对于识别可改变的风险是必不可少的 认知下降的因素，并提供新的干预措施。我们将招募150名拉美裔中年成年人 交错招聘和利用我们现有的资源：总共进行了450次观察。每位参与者 将接受一系列神经认知和睡眠评估，并将提供客观的睡眠数据 通过手腕佩戴的肌动记录仪，在一天内采集3份唾液样本，以评估唾液皮质醇和大便 通过全基因组鸟枪测序来检查肠道微生物群的样本。使用这些数据，我们将 检查四个目标：目标1.使用综合微生物组分析，我们将检查肠道之间的相关性 微生物组概况，以及认知的测量。目标2.我们将检验以下两个变量之间的关系 睡眠和肠道微生物群谱。目标3a：我们将研究睡眠与以下措施之间的关系 认知力。目标3b：我们将检查肠道微生物群组之间的相互联系，测量 认知和睡眠使用两个中介模型。目标4：我们将检查肠道之间的相互关系 微生物群谱、压力(唾液皮质醇)、认知测量和睡眠。使用综合分析 肠道微生物群，认知，通过唾液皮质醇的压力，和睡眠测量，我们的研究将是 在最早探索肠道微生物群多样性、微生物谱、压力、认知、 和睡眠，以进行未来生活过程的纵向研究，并了解随着时间的变化的影响。

FIU-RCMI Research Project #2 Project Summary Emerging evidence suggests a bidirectional relationship between sleep disturbance and alterations in the gut microbiome. This study aims to examine the effect of sleep health and dysfunction in influencing cognition while accounting for the role of the gut microbiome in a community-based Latinx sample. Despite its importance, studies exploring the effect of sleep on the gut microbiome, and the subsequent effect on the cognition of humans are extremely limited. Of the preliminary investigations that do exist, none have been conducted with Latinx community-living samples. While Latinx samples were the focus of two gut microbiome studies, which provided preliminary evidence for the unique gut microbiome of this population, our proposed study is innovative in that it includes cognitive measures, cortisol sampling, and objective measures of sleep. Guided by the National Institutes of Minority Health and Health Disparities Framework, we will also explore sociocultural factors at various levels of influence that may impact sleep, cognition, and the gut microbiome. There is an urgent need for studies of this kind given that the gut microbiome may have a direct effect on cognition and sleep, positioning it as a modifiable risk factor for cognitive decline. Sleep disturbance, a modifiable risk factor for cognitive decline, including Alzheimer's disease, is bidirectionally-related to the gut microbiome, variations in sleep patterns affect the structure and diversity of the gut microbiome, and the gut microbiome is influential in circadian cycles and hormones related to sleep and waking. Investigations examining the microbiota-gut-brain axis are essential in community-based samples to identify modifiable risk factors for cognitive decline and inform novel interventions. We will recruit 150 Latinx midlife adults through staggered recruitment and leveraging our existing resources: for a total of 450 observations. Each participant will be administered a battery of neurocognitive and sleep assessments and will provide objective sleep data through a wrist-worn actigraphy device, 3 saliva samples in one day to assess salivary cortisol, and a stool sample to examine the gut microbiome through whole-genome shotgun sequencing. Using this data, we will examine four aims: Aim 1. Using integrated microbiome analyses, we will examine correlations between gut microbiome profiles, and measures of cognition. Aim 2. We will examine the bivariate relationship between sleep and gut microbiome profiles. Aim 3a: We will examine the relation between sleep and measures of cognition. Aim 3b: We will examine the interconnections between gut microbiome profiles, measures of cognition, and sleep using two mediation models. Aim 4: We will examine the interrelations between gut microbiome profiles, stress (salivary cortisol), measures of cognition, and sleep. Using an integrated analysis of the gut microbiome, cognition, stress through salivary cortisol, and sleep measurements, our study will be among the first to explore the interrelations of gut microbiome diversity, microbial profiles, stress, cognition, and sleep to conduct future life course longitudinal studies and understand the changing effects over time.