Equipment Fund

预算理由-协作发展基金 我们要求每年提供135 000美元，以设立一个协作发展基金。 将被招募，以帮助进一步确定和验证艾滋病毒宿主蛋白复合物，促进结构， 确定复合物，利用结构信息更深入地探索生物功能， HIV调节蛋白和辅助蛋白，以及高通量方面的扩展技术发展 表达、结构测定、MS方法、冷冻EM和断层扫描。一种方法是 促进必要的合作将是为有前途的研究提供种子资金。这些研究可能 包括试剂制备、诱变、生物化学、功能或病毒学实验， 识别和验证新的复合物，或其他方法的开发。申请的基金将提供 灵活从事新的方向，并将主要用于资助与外部调查员的分包合同， 经与我们的咨询委员会协商并经NIGMS批准后确定。本类别基金 此外，还将用于现有和潜在合作者访问HARC中心，以规划未来的研究 方向

BUDGET JUSTIFICATION - Collaboration Development Fund We request $135,000 per year to establish a Collaboration Development Fund. A wide range of collaborators will be enlisted to help further identify and validate HIV-host protein complexes, facilitate structure determination of the complexes, utilize structural information to more deeply probe the biological functions of the HIV regulatory and accessory proteins, and extend technological developments in high-throughput expression, structure determination, MS methods, cryo-EM, and tomography. One way in which we will facilitate essential collaborations will be to provide seed funding for promising studies. These studies may include reagent preparation, mutagenesis, biochemical, functional, or virological experiments, methods to identify and validate new complexes, or other methods development. The requested Fund will provide flexibility to engage in new directions and will be used primarily to fund sub-contracts with outside investigators, as determined in consultation with our advisory committees and with NIGMS approval. Funds in this category also will be allocated to visits of existing and potential collaborators to the HARC Center to plan future research directions.