Cell Differentation During Decidualization

蜕膜化过程中的细胞分化

• 批准号: 7650592
• 负责人: ZUZANA STRAKOVA
• 金额: $ 49.46万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7650592
• 关键词: Actin-Binding Protein; Actins; Affect; Age; Architecture; Biochemical; Cell Nucleus; Cell Proliferation; Cells; Conceptions; Cyclic AMP; Cytoskeletal Modeling; Cytoskeleton; DNA Sequence Rearrangement; Decidual Cell; Decidual Cell Reactions; Defect; Diagnosis; Disease; Elements; Embryo; Endometrium; Estrogens; Event; Fibroblasts; Gene Expression; Goals; Health; Human; In Vitro; Individual; Infertility; Interleukin-12; Intervention; Knowledge; Mediator of activation protein; Modeling; Modification; Molecular; Papio; Patients; Pregnancy Maintenance; Primates; Process; Progesterone; Property; Regulation; Research; Role; Scheme; Stimulus; Stromal Cells; Testing; Transcriptional Coactivator with PDZ-Binding Motif; Woman; base; cofilin; cytokine; endometriosis; in vivo; nonhuman primate; public health relevance; reproductive; response; steroid hormone

Decidualization, which involves differentiation of stromal fibroblasts into decidual cells, is a major change that occurs in the primate endometrium after conception. Decidualization is critical for the establishment and maintenance of pregnancy. Based on our previous in vitro and in vivo results, we have proposed cytokine interleukin-1β (IL-1β) as one of the possible mediators of the decidualization process (1,2,3). We demonstrated that in vitro decidualization induced by the embryonic stimulus IL-1β or the widely established exogenous stimulus cAMP (both in the presence of E and P) is accompanied by extensive changes in the cytoskeletal architecture (6). Our data provide evidence that cytoskeletal dynamics (actin-myosin interactions), rather than the absolute level of cytoskeletal stiffness or the precise organization of the cytoskeleton, are the more important elements of decidualization (6). Despite extensive research, the etiology and pathophysiology of endometriosis and its relationship to decreased fecundity still remain unclear. Defects in the decidualization process were described in endometriosis and infertility patients (11,12,13). We hypothesize that the ability of stromal cells to re-organize the cytoskeleton, a process that leads to decidualization, is compromised in patients with endometriosis.

涉及将基质成纤维细胞分化为decIDual细胞的c典型化是主要的 概念之后，灵长类动物子宫内膜发生的变化。义词化对 妊娠的建立和维持。根据我们以前的体外和体内结果，我们 已经提出了细胞因子白介素1β（IL-1β）作为决定的可能介体之一 过程（1,2,3）。我们证明了体外 由胚胎刺激IL-1β或 广泛建立的外源刺激营（都在 E和P）的存在是通过广泛完成的 细胞骨架结构的变化（6）。我们的数据 提供了细胞骨架动力学（肌动蛋白 - 肌球蛋白）的证据 相互作用），而不是细胞骨架的绝对水平 刚度或细胞骨架的精确组织， 是决定的更重要的要素（6）。 尽管进行了广泛的研究，病因和 子宫内膜异位症的病理生理及其与 繁殖力的缺陷仍然不清楚 在子宫内膜异位中描述了决定性化过程 和不育患者（11,12,13）。我们假设 基质细胞重组细胞骨架的能力，A 导致决策的过程被妥协 子宫内膜异位症患者。