New Amphiphilic Dendrimers for Encapsulation and Release

用于封装和释放的新型两亲性树枝状聚合物

• 批准号: 7656056
• 负责人: Sankaran Thayumanavan
• 金额: $ 28.98万
• 依托单位: UNIVERSITY OF MASSACHUSETTS AMHERST
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7656056
• 关键词: Address; Area; Back; Basic Science; Binding; Biological Process; Biology; Cell Communication; Cell physiology; Cell surface; Cells; Chemicals; Custom; Dendrimers; Development; Drug Delivery Systems; Dyes; Electron Microscopy; Environment; Enzymes; Equilibrium; Event; Fibroblast Growth Factor; Funding; Goals; Head; Heparin; Inorganic Sulfates; Ligands; Literature; Location; Medicine; Micelles; Modification; Nature; Pharmaceutical Preparations; Polymers; Polysaccharides; Process; Property; Proteins; Reaction; Receptor Protein-Tyrosine Kinases; Reporting; Signal Transduction; Spectrum Analysis; Stimulus; Structure; System; Unspecified or Sulfate Ion Sulfates; Water; base; controlled release; design; driving force; human disease; interest; light scattering; macromolecular assembly; macromolecule; receptor; research study; response; scaffold

Assembly and disassembly of proteins, nature's macromolecules, in response to a specific stimulus is ubiquitous in biology. Mimicking these processes, where proteins are used as the stimulus to disassemble artificial macromolecular assemblies are interesting for a variety of biomedical applications. For example, although targeted drug delivery is not a specific goal for this proposal, the experiments proposed here to obtain the proof-of-concept could have a significant impact in this area. Specifically, this proposal describes the development of versatile strategies to disassemble dendrimer-based amphiphilic assemblies using proteins as stimuli. Facially amphiphilic dendrimers, reported by us recently in the literature, are a unique class of molecules in that these are the only class of amphiphilic dendrimers that form micelle-type assemblies through aggregation. Even more interesting is the fact that these assemblies can be disassembled in response to a stimulus. While stimuli-responsive assemblies themselves have been studied quite extensively, reports where proteins are used as the stimulus for disassembling organized supramolecular assemblies are rather limited. This proposal describes a concerted approach to this problem, which could have broad implications in biology and medicine.

蛋白质（自然界的大分子）响应特定刺激的组装和分解在生物学中无处不在。模仿这些过程，其中蛋白质被用作分解人工大分子组装体的刺激物，对于各种生物医学应用都很有趣。例如，虽然靶向药物输送不是该提案的具体目标，但此处提出的用于获得概念验证的实验可能会在该领域产生重大影响。具体来说，该提案描述了使用蛋白质作为刺激物来拆卸基于树枝状大分子的两亲组装体的通用策略的开发。我们最近在文献中报道的表面两亲性树枝状聚合物是一类独特的分子，因为它们是唯一一类通过聚集形成胶束型组装体的两亲性树枝状聚合物。更有趣的是，这些组件可以根据刺激而被拆卸。虽然刺激响应组装体本身已经得到相当广泛的研究，但使用蛋白质作为分解有组织的超分子组装体的刺激物的报道相当有限。该提案描述了解决该问题的协调方法，可能在生物学和医学领域产生广泛的影响。