Core D: Neuropathology and Animal Behavior

核心 D：神经病理学和动物行为

• 批准号: 7559782
• 负责人: ELIEZER MASLIAH
• 金额: $ 12.5万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-15 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7559782
• 关键词: Alzheimer' s Disease; Animal Behavior; Animal Model; Anterior; Apoptosis; Arts; Autopsy; Behavioral; Biochemical; Biological Preservation; Brain; Brain Stem; Brain region; Cell model; Characteristics; Chromosome Pairing; Clinical; Cognitive; Complex; Condition; Consensus; Consultations; DLG4 gene; Data; Dementia; Detergents; Development; Diagnosis; Disease; Drosophila genus; Equilibrium; Experimental Designs; Freezing; Fresh Tissue; Genes; Glial Fibrillary Acidic Protein; Guidelines; Hippocampus (Brain); Housing; Human; Immunoblotting; In Vitro; Injection of therapeutic agent; Investigation; Laboratories; Lentivirus Vector; Lewy Body Disease; Microtubule-Associated Protein 2; Mission; Modeling; Modification; Molecular; Motor; Mus; Mutation; Necrosis; Neocortex; Nerve Degeneration; Neurons; Parkinson Disease; Parkinson' s Dementia; Parkinsonian Disorders; Pathogenesis; Pathologic; Pathway interactions; Patients; Pattern; Performance; Phosphorylation; Phosphotransferases; Platelet-Derived Growth Factor; Play; Principal Investigator; Process; Protein Overexpression; Proteins; Purpose; RNA; Research; Research Support; Role; Saccharin; Sampling; Self Administration; Staging; Staining method; Stains; Synapses; Synaptophysin; Techniques; Testing; Time; Tissues; Transgenic Animals; Transgenic Organisms; Tyrosine 3-Monooxygenase; Visual; brain tissue; calbindin; density; dopamine transporter; embryonic stem cell; human embryonic stem cell; human tissue; in vitro Model; in vivo; leucine-rich repeat kinase 2; motor learning; mouse model; mutant; neocortical; nerve stem cell; neuropathology; parkin gene/protein; programs; promoter; protein aggregate; protein misfolding; protein protein interaction; small molecule; synuclein; therapy development; thioflavine; tissue fixing

The mission of the Neuropathology and Animal Behavior Core will be to provide the projects of the NIEHS Center with well-characterized autopsy brain material from human PD cases as well as with transgenic (tg) animal models expressing a-synuclein (a-syn), DJ-1, Parkin and PINK1 for neuropathological, molecular, biochemical and behavioral studies. The Core will provide support with human tissues from PD patients and animal models of PD to Projects 1, 2, and 3; support behavioral studies in Project 3; and help with survival/apoptosis/necrosis analysis of human embryonic stem cell (hESC)-derived developing neurons for Project 4. The Core has developed state of the art techniques for neuropathological analysis of neurodegeneration, biochemical analysis of misfolded protein accumulation, and behavioral analysis of motor and cognitive alterations. Moreover, we have developed unique animal models of PD in which to test and screen new molecular pathways and small molecules. Please note that a human clinical component, as defined in the NIEHS Center RFA, is contained within this Core.

神经病理学和动物行为核心的使命是提供以下项目： NIEHS中心也有来自人类PD病例的特征良好的尸检大脑材料 与表达α-突触核蛋白（α-syn）、DJ-1、Parkin和PINK 1的转基因（tg）动物模型一样 用于神经病理学、分子学、生物化学和行为学研究。核心将提供 为项目1、2和3提供PD患者和PD动物模型的人体组织支持; 支持项目3中的行为研究;并帮助进行存活/凋亡/坏死分析， 人胚胎干细胞（hESC）衍生的发育中的神经元项目4。核心 开发了用于神经变性的神经病理学分析的最新技术， 错误折叠蛋白质积累的生化分析，以及运动和 认知改变此外，我们还开发了独特的PD动物模型， 筛选新的分子途径和小分子。请注意，人类临床 NIEHS中心RFA中定义的组件包含在本核心中。