Neurobiology Core

神经生物学核心项目总结/摘要：神经生物学核心的总体目标 (NB)是为NeuroAIDS社区提供一套神经生物学资源， 分析和发现与HIV延长生存期相关的神经变性机制 感染，从全面和动态的角度。NB Core已重新组织为In Vivo 和体外单位，以更好地服务于当前和未来的neuroAIDS领域的需求。主资源 目标是：（a）提供一套创新的最先进的神经病理学和神经生物学方法， 支持病毒持续存在/根除机制研究的体内和体外资源， 神经炎症中的微生物组和衰老在HIV感染的神经变性中的作用，（B）鼓励 并促进针对中心的这些和其他科学主题的合作工作，以及（c）提供 为新的研究者提供用户培训和咨询。 此外，NB核心科学目标将包括：（1）为艾滋病毒感染研究提供支持 在中枢神经系统，解决病毒的持久性和根除，包括染色质修饰剂的分子研究， 表观遗传标记和病毒产生和循环的标记;（2）提供体外和体内 支持微生物组主题的神经病理学资源和分析，其中可能包括以下研究： HAND患者的神经炎症、神经血管单位损伤和肠道病理学模式;（3） 提供艾滋病毒相关神经病理学的新集合的定量分析，以支持老龄化的主题。 关于衰老主题的工作的例子可能包括以下神经病理学和生物化学研究： 蛋白质（Aβ）沉积，包括脑淀粉样血管病; Tau和α-突触核蛋白积聚; 自噬和溶酶体活化;线粒体生物发生和自噬标记物;脑亲免素 反应;以及人原代神经胶质细胞、脑内皮细胞和血管平滑肌细胞培养物。和 最后，（4）我们将继续支持临床病理学研究，研究 神经退行性疾病和手的新标志物。 了解衰老背景下病毒持续存在和神经损伤的神经病理学基础 和微生物组作为促成因素，将阐明艾滋病毒导致HAND的机制， 为这种致残性疾病提供新的治疗方法。

NEUROBIOLOGY CORE PROJECT SUMMARY/ABSTRACT: The overall objective of the Neurobiology Core (NB) is to provide the NeuroAIDS community with a set of neurobiological resources that will enhance the analysis and discovery of the mechanisms of neurodegeneration associated with prolonged survival with HIV infection, from a comprehensive and dynamic perspective. The NB Core has been re-organized into In Vivo and In Vitro Units to better serve the current and future needs of the neuroAIDS field. The main resource objectives will be to: (a) provide a set of innovative state-of-the-art neuropathological and neurobiological in vivo and in vitro resources to support studies of the mechanisms of viral persistence/eradication, influence of microbiome in neuro-inflammation and role of aging in neurodegeneration with HIV infection, (b) encourage and facilitate collaborative work addressing these and other scientific themes of the Center, and (c) provide user training and consultation to new investigators. In addition, the NB Core Scientific objectives will include: (1) To provide support for studies of HIV infection in the CNS that address viral persistence and eradication, including molecular studies of chromatin modifiers, epigenetic markers and markers of viral production and cycling; (2) To provide in vitro and in vivo neuropathological resources and assays in support of the Microbiome theme, that could include studies of patterns of neuro-inflammation, neuro-vascular unit injury and gut pathology in patients with HAND; (3) To provide quantitative analysis of novel sets of HIV related neuropathologies in support of the theme on Aging. Examples of work on the Aging theme could include neuropathological and biochemical studies of: amyloid-β protein (Aβ) deposits including cerebral amyloid angiopathy; Tau and α-synuclein accumulation; markers of autophagy and lysosomal activation; markers of mitochondrial biogenesis and mitophagy; brain immunophilin response; and human primary neuro-glial, brain endothelial and vascular smooth muscle cell cultures. And finally, (4) we will continue supporting clinico-pathological studies which investigate the relationship between new markers of neurodegeneration and HAND. Understanding the neuropathological basis for viral persistence and neural injury in the context of aging and the microbiome as contributing factors, will elucidate mechanisms through which HIV leads to HAND and inform new treatments for this disabling condition.