Neurobiology Core

神经生物学核心项目摘要/摘要：神经生物学核心的总体目标 HIV感染者存活时间延长与神经退行性变机制的分析与发现 感染，从全面和动态的角度来看。NB核心已在Vivo中重组为 和体外单位，以更好地服务于神经艾滋病领域当前和未来的需求。主要资源 目标将是：(A)提供一套创新的最先进的神经病理学和神经生物学 支持病毒持续/根除机制研究的体内和体外资源， 微生物组在神经炎症中的作用和衰老在艾滋病毒感染的神经变性中的作用，(B)鼓励 并促进针对中心的上述主题和其他科学主题的协作工作，以及(C)提供 对新的调查人员进行用户培训和咨询。 此外，国家实验室的核心科学目标将包括：(1)为艾滋病毒感染研究提供支助 在涉及病毒持久性和根除的中枢神经系统中，包括染色质修饰物的分子研究， 表观遗传标记和病毒产生和循环的标记；(2)提供体外和体内 支持微生物组主题的神经病理资源和分析，其中可能包括对 手部疾病患者的神经炎症、神经血管单位损伤和肠道病理类型； 关于衰老主题的工作的例子可能包括：淀粉样蛋白-β的神经病理和生化研究 以及人类原代神经胶质细胞、脑内皮细胞和血管平滑肌细胞培养。和 最后，(4)我们将继续支持临床病理研究，以调查 神经退行性变和手的新标记物。 在衰老的背景下理解病毒持续和神经损伤的神经病理学基础 和微生物组作为促进因素，将阐明艾滋病毒通过其导致手部和 通知这种致残情况的新治疗方法。

NEUROBIOLOGY CORE PROJECT SUMMARY/ABSTRACT: The overall objective of the Neurobiology Core (NB) is to provide the NeuroAIDS community with a set of neurobiological resources that will enhance the analysis and discovery of the mechanisms of neurodegeneration associated with prolonged survival with HIV infection, from a comprehensive and dynamic perspective. The NB Core has been re-organized into In Vivo and In Vitro Units to better serve the current and future needs of the neuroAIDS field. The main resource objectives will be to: (a) provide a set of innovative state-of-the-art neuropathological and neurobiological in vivo and in vitro resources to support studies of the mechanisms of viral persistence/eradication, influence of microbiome in neuro-inflammation and role of aging in neurodegeneration with HIV infection, (b) encourage and facilitate collaborative work addressing these and other scientific themes of the Center, and (c) provide user training and consultation to new investigators. In addition, the NB Core Scientific objectives will include: (1) To provide support for studies of HIV infection in the CNS that address viral persistence and eradication, including molecular studies of chromatin modifiers, epigenetic markers and markers of viral production and cycling; (2) To provide in vitro and in vivo neuropathological resources and assays in support of the Microbiome theme, that could include studies of patterns of neuro-inflammation, neuro-vascular unit injury and gut pathology in patients with HAND; (3) To provide quantitative analysis of novel sets of HIV related neuropathologies in support of the theme on Aging. Examples of work on the Aging theme could include neuropathological and biochemical studies of: amyloid-β protein (Aβ) deposits including cerebral amyloid angiopathy; Tau and α-synuclein accumulation; markers of autophagy and lysosomal activation; markers of mitochondrial biogenesis and mitophagy; brain immunophilin response; and human primary neuro-glial, brain endothelial and vascular smooth muscle cell cultures. And finally, (4) we will continue supporting clinico-pathological studies which investigate the relationship between new markers of neurodegeneration and HAND. Understanding the neuropathological basis for viral persistence and neural injury in the context of aging and the microbiome as contributing factors, will elucidate mechanisms through which HIV leads to HAND and inform new treatments for this disabling condition.