Large Animal Core (Core 1)

大型动物核心（核心1）

• 批准号: 10713242
• 负责人: Beth A Costine-Bartell
• 金额: $ 71.36万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10713242
• 关键词: Acute; Adhesions; Animal Husbandry; Animal Model; Animals; Archives; Bilateral; Blood; Brain; Calcium; Chlorides; Collection; Convulsions; Data; Dyes; Electrodes; Electroencephalography; Epilepsy; Family suidae; Housing; Image; Implant; Injections; Injury; Membrane; Microscopy; Modeling; Monitor; Morphologic artifacts; Movement; Neurons; Noise; Operative Surgical Procedures; Polypropylenes; Post-Traumatic Epilepsy; Problem Solving; Procedures; Proteins; Reporting; Resources; Signal Transduction; Site; Technology; Time; Tissues; Video Recording; battery life; cellular imaging; cranium; digital; experience; extracellular; flexibility; improved; meetings; porcine model; prevent; screening; serial imaging; tissue regeneration; two-photon

Large Animal Core Abstract We have developed the first validated, gyrencephalic, large animal model of post- traumatic epilepsy achieving extreme, long-term video EEG digital radiotelemetry, and we have developed cutting-edge technology allowing single cell imaging in the largest species to date. Building on our 5 years’ experience, the Large Animal Core aims to transduce the cortex of swine with or without post-traumatic epilepsy with proteins that report intraneuronal concentrations of chloride and calcium, surgically implant and maintain imaging ports for the Microscopy Core allowing for serial, 2-photon imaging, and produce and archive video/electrographic data and collect and store tissues of swine with or without post-traumatic epilepsy. In particular, the Large Animal Core will provide surgeries (implant the ECoG, AAV injection, install the imaging ports, cortical impact, inject extracellular chloride dye, 2-photon imaging), record video ECoG with real-time analysis, collect tissues, animal husbandry procedures, regulatory compliance, and facilitation of swine housing resources. The Core Director and staff will participate in weekly meetings regarding administrative and scientific matters such as any problem solving any issues that arise, convulsion screening, and presentations of data.

大型动物核心摘要 我们已经开发了第一个经过验证的，脑回，大动物模型后， 创伤性癫痫实现极端，长期视频脑电图数字无线电遥测，我们有 开发了尖端技术，允许迄今为止最大物种的单细胞成像。 基于我们5年的经验，大型动物核心旨在将大脑皮层 有或没有创伤后癫痫的猪，具有报告神经元内的蛋白质 氯化物和钙的浓度，手术植入和维持成像端口， 显微镜核心允许串行，双光子成像，并产生和存档 视频/电图数据，并收集和存储有或没有创伤后的猪的组织 癫痫特别是，大型动物中心将提供手术（植入ECoG、AAV 注射，安装成像端口，皮质撞击，注射细胞外氯化物染料，双光子 成像），记录实时分析的视频ECoG，收集组织，动物饲养 程序、法规遵从性和猪舍资源的便利化。核心 主任和工作人员将参加有关行政和科学的每周会议 诸如解决出现的任何问题、惊厥筛查和 数据的介绍。