Large Animal Core (Core 1)

大型动物核心（核心1）

• 批准号: 10713242
• 负责人: Beth A Costine-Bartell
• 金额: $ 71.36万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10713242
• 关键词: Acute; Adhesions; Animal Husbandry; Animal Model; Animals; Archives; Bilateral; Blood; Brain; Calcium; Chlorides; Collection; Convulsions; Data; Dyes; Electrodes; Electroencephalography; Epilepsy; Family suidae; Housing; Image; Implant; Injections; Injury; Membrane; Microscopy; Modeling; Monitor; Morphologic artifacts; Movement; Neurons; Noise; Operative Surgical Procedures; Polypropylenes; Post-Traumatic Epilepsy; Problem Solving; Procedures; Proteins; Reporting; Resources; Signal Transduction; Site; Technology; Time; Tissues; Video Recording; battery life; cellular imaging; cranium; digital; experience; extracellular; flexibility; improved; meetings; porcine model; prevent; screening; serial imaging; tissue regeneration; two-photon

Large Animal Core Abstract We have developed the first validated, gyrencephalic, large animal model of post- traumatic epilepsy achieving extreme, long-term video EEG digital radiotelemetry, and we have developed cutting-edge technology allowing single cell imaging in the largest species to date. Building on our 5 years’ experience, the Large Animal Core aims to transduce the cortex of swine with or without post-traumatic epilepsy with proteins that report intraneuronal concentrations of chloride and calcium, surgically implant and maintain imaging ports for the Microscopy Core allowing for serial, 2-photon imaging, and produce and archive video/electrographic data and collect and store tissues of swine with or without post-traumatic epilepsy. In particular, the Large Animal Core will provide surgeries (implant the ECoG, AAV injection, install the imaging ports, cortical impact, inject extracellular chloride dye, 2-photon imaging), record video ECoG with real-time analysis, collect tissues, animal husbandry procedures, regulatory compliance, and facilitation of swine housing resources. The Core Director and staff will participate in weekly meetings regarding administrative and scientific matters such as any problem solving any issues that arise, convulsion screening, and presentations of data.

大型动物核心摘要 我们已经建立了第一个经过验证的后脑回大型动物模型。 创伤性癫痫达到极端，长期视频脑电数字无线电遥测，我们有 开发了尖端技术，可以对迄今为止最大的物种进行单细胞成像。 基于我们5年的经验，大型动物核心的目标是转导大脑皮质 患有或不患有创伤后癫痫的猪，其蛋白质报告在神经元内 氯化物和钙的浓度，通过手术植入和维护成像端口 允许连续、双光子成像、产生和存档的显微镜核心 有或无创伤后猪的视频/电信号数据以及收集和存储组织 癫痫。特别是，大动物核心将提供手术(植入ECoG，AAV 注射，安装成像端口，皮质撞击，注射细胞外氯化物染料，双光子 成像)，记录实时分析的视频ECoG，收集组织，畜牧业 猪舍资源的程序、法规遵从性和便利化。《核心》 主任和工作人员将参加每周一次的行政和科学会议 解决任何问题所产生的任何问题、惊厥筛查和 数据的表示。