Microbial Genomics and Analytical Laboratory (MGAL) Core

项目摘要-微生物基因组学和分析实验室(MGAl) 环境综合中心的微生物基因组和分析实验室(MGAl)核心 微生物与人类健康在华盛顿大学提供集中的设施和技术专长 Hawai‘i at MāNoa(UHM)，以支持微生物环境与人类之间的界面研究 健康。高通量测序技术在微生物群落组成研究中的应用 代谢物分析是用于微生物组研究领域的标准技术。镁铝芯 通过提供下列服务，使人们能够使用这些关键方法：(1)基因组制备服务 文库和基因表达的量化；(2)高效液相色谱和质谱法 用于分析脂质和代谢物的光谱分析仪器；和(3)培养设施， 微生物菌株的分离、长期保存。在扩大核心功能和用户基础的同时 继续支持目前和过去的Cobre研究人员，MGAl服务将扩展到包括 元基因组和转录文库的制备、代谢组学分析和增强型微生物 生物库设施，包括创建一个包含信息的社区可搜索数据库 关于夏威夷的地方性菌株。夏威夷全州的研究人员都可以使用镁铝核心 和来访的科学家，从而鼓励当地微生物组研究社区内更多的互动 以及来自海外的合作努力的种子。该设施将有益于影响Cobre调查人员和 通过提供必要的基础设施和专业知识来推进研究和促进 初级教员。此外，核心将支持UHM作为主要微生物组中心的持续发展 通过提供图书馆准备和代谢物分析方面的实践培训，在太平洋区域开展研究， 举办关于基础微生物组研究方法的夏季研讨会，与本科生和 研究生实验室课程，并促进推广工作，以确定夏威夷的微生物群的特征。

PROJECT SUMMARY – Microbial Genomics and Analytical Laboratory (MGAL) The Microbial Genomics and Analytical Laboratory (MGAL) Core of the Integrative Center for Environmental Microbiomes and Human Health provides a centralized facility and technical expertise at the University of Hawai‘i at Mānoa (UHM) to support the study of the interface between the microbial environment and human health. The characterization of microbiota community composition by high throughput sequencing and metabolite analysis are standard techniques used in the field of microbiome research. The MGAL Core enables access to these key methods by providing the following: (i) services for the preparation of genomic libraries and quantification of gene expression; (ii) high-performance liquid chromatography and mass spectrometry instrumentation for the analysis of lipids and metabolites; and (iii) facilities for the cultivation, isolation, and long-term storage of microbial strains. To expand Core capabilities and user base while continuing to support current and past COBRE researchers, MGAL services will be expanded to include metagenomic and transcriptomic library preparation, metabolomic analysis, and enhanced microbial biobanking facilities that will include the creation of a community searchable database containing information about endemic Hawaiian strains. The MGAL Core is available to researchers throughout the state of Hawai'i and visiting scientists, thus encouraging greater interaction within the local microbiome research community and seeding collaborative efforts from abroad. The facility will beneficially impact COBRE investigators and affiliates by providing essential infrastructure and expertise to advance research and foster the careers of junior faculty. Moreover, the Core will support the continued growth of UHM as a major center for microbiome studies in the Pacific Region by providing hands-on training in library preparation and metabolite analysis, hosting summer workshops on fundamental microbiome research methods, integrating with undergraduate and graduate laboratory classes, and facilitating outreach efforts to characterize Hawai'i’s microbiome.