Microbial Genomics and Analytical Laboratory (MGAL) Core

微生物基因组学和分析实验室 (MGAL) 核心

• 批准号: 10712774
• 负责人: JOANNE Y YEW
• 金额: $ 27.42万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-15 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10712774
• 关键词: Acceleration; Aerobic; Area; Award; Bioinformatics; Biology; Biomedical Research; Centers of Research Excellence; Communities; Complement; Data; Destinations; Development; Disease; Doctor of Philosophy; Ecosystem; Education; Educational workshop; Ensure; Environment; Equipment; Faculty; Fostering; Gene Expression; Genomic Library; Genomics; Goals; Grant; Growth; Hawaii; Hawaiian; Health; High Pressure Liquid Chromatography; High-Throughput Nucleotide Sequencing; Human; Infrastructure; Internships; Laboratories; Laboratory Study; Libraries; Lipids; Mass Spectrum Analysis; Metabolic; Metagenomics; Methods; Microbe; Microbiology; Modernization; Molecular Biology; Native Hawaiian; Phase; Preparation; Privatization; Proteomics; Publications; Qualifying; Research; Research Methodology; Research Personnel; Research Support; Resolution; Resources; Sampling; Scientist; Secure; Services; Shotguns; Site; Students; Taxus; Technical Expertise; Techniques; Technology; Time; Training; Universities; Virus; Visit; analytical method; base; biobank; career; data analysis pipeline; experience; instrumentation; interest; laboratory facility; liquid chromatography mass spectrometry; metabolomics; microbial; microbial genomics; microbiome; microbiome research; microbiota; next generation; normal microbiota; outreach; programs; recruit; searchable database; small molecule; success; tool; training opportunity; transcriptomics; undergraduate student; user-friendly

PROJECT SUMMARY – Microbial Genomics and Analytical Laboratory (MGAL) The Microbial Genomics and Analytical Laboratory (MGAL) Core of the Integrative Center for Environmental Microbiomes and Human Health provides a centralized facility and technical expertise at the University of Hawai‘i at Mānoa (UHM) to support the study of the interface between the microbial environment and human health. The characterization of microbiota community composition by high throughput sequencing and metabolite analysis are standard techniques used in the field of microbiome research. The MGAL Core enables access to these key methods by providing the following: (i) services for the preparation of genomic libraries and quantification of gene expression; (ii) high-performance liquid chromatography and mass spectrometry instrumentation for the analysis of lipids and metabolites; and (iii) facilities for the cultivation, isolation, and long-term storage of microbial strains. To expand Core capabilities and user base while continuing to support current and past COBRE researchers, MGAL services will be expanded to include metagenomic and transcriptomic library preparation, metabolomic analysis, and enhanced microbial biobanking facilities that will include the creation of a community searchable database containing information about endemic Hawaiian strains. The MGAL Core is available to researchers throughout the state of Hawai'i and visiting scientists, thus encouraging greater interaction within the local microbiome research community and seeding collaborative efforts from abroad. The facility will beneficially impact COBRE investigators and affiliates by providing essential infrastructure and expertise to advance research and foster the careers of junior faculty. Moreover, the Core will support the continued growth of UHM as a major center for microbiome studies in the Pacific Region by providing hands-on training in library preparation and metabolite analysis, hosting summer workshops on fundamental microbiome research methods, integrating with undergraduate and graduate laboratory classes, and facilitating outreach efforts to characterize Hawai'i’s microbiome.

微生物基因组学和分析实验室（MGAL） 微生物基因组学和分析实验室（MGAL）环境综合中心的核心 微生物组和人类健康提供了一个集中的设施和技术专长在大学 Hawai 'i at Mānoa（UHM）支持微生物环境与人类之间界面的研究 健康通过高通量测序表征微生物群群落组成， 代谢物分析是微生物组研究领域中使用的标准技术。MGAL核心 通过提供以下服务，使人们能够获得这些关键方法：（i）制备基因组 文库和基因表达的定量;（ii）高效液相色谱和质谱 用于分析脂质和代谢物的光谱仪器;和（iii）用于培养的设施， 微生物菌株的分离和长期储存。扩大核心能力和用户群，同时 MGAL将继续支持当前和过去的COBRE研究人员，其服务范围将扩大到包括 宏基因组和转录组文库制备、代谢组学分析和增强的微生物 生物库设施，包括创建一个社区可搜索的数据库， 关于夏威夷地方性病毒株MGAL核心可供整个夏威夷州的研究人员使用 和访问科学家，从而鼓励当地微生物组研究界之间的更多互动 并从国外引进合作成果。该设施将对COBRE调查人员产生有利影响， 通过提供必要的基础设施和专业知识，以推进研究和促进职业生涯的分支机构 初级教员。此外，核心将支持UHM作为微生物组主要中心的持续增长 通过提供文库制备和代谢物分析方面的实践培训， 举办关于基本微生物组研究方法的夏季研讨会，与本科生和 研究生实验室课程，并促进外展工作，以表征夏威夷的微生物组。