Microbial Genomics and Analytical Laboratory (M-GAL)

项目摘要-CORE C：微生物基因组学和分析实验室(M-GAL) 环境综合中心的微生物基因组和分析实验室(M-GAL)核心 微生物与人类健康将在华盛顿大学提供一个集中的设施和技术专长 夏威夷MāNoa(UHM)支持研究微生物环境与人类之间的界面 健康。用下一代测序和分析方法表征微生物群落组成 微生物代谢产物的分析是微生物组研究领域使用的标准方法。要启用 在这些应用中，M-GAL核心将提供以下内容：(1)准备工作所需的设备和专门知识 基因组文库和基因表达的定量；(Ii)高效液相色谱法和 用于分析脂质和代谢物的质谱仪；(3)培养设施， 微生物菌株的分离和长期储存；以及(4)管理一个社区可检索的数据库 包含从夏威夷本土动物和生态系统分离的菌株的信息。M-GAL核心 将提供给夏威夷全州的研究人员和来访的科学家，从而鼓励更大的 当地微生物研究界之间的互动和来自国外的合作努力的种子。 M-GAL Core将通过为Cobre调查人员和附属公司提供必要的 基础设施和专业知识，以促进他们的研究和培养初级教师的职业生涯。此外， CORE将通过以下方式支持UHM发展成为太平洋地区微生物组研究的主要中心 提供图书馆准备和代谢物分析方面的实践培训，主办国际夏季活动 关于基础微生物组研究方法的学校，并促进推广工作，以确定 夏威夷的微生物群。 好了！

Project Summary – CORE C: Microbial Genomics and Analytical Laboratory (M-GAL) The Microbial Genomics and Analytical Laboratory (M-GAL) Core of the Integrative Center for Environmental Microbiomes and Human Health will provide a centralized facility and technical expertise at the University of Hawai`i at Mānoa (UHM) to support the study of the interface between the microbial environment and human health. The characterization of microbial community composition by next-generation sequencing and analysis of microbial metabolic products are standard methods used in the field of microbiome research. To enable these applications, the M-GAL Core will provide the following: (i) equipment and expertise for the preparation of genomic libraries and the quantitation of gene expression; (ii) high-performance liquid chromatography and mass spectrometry instrumentation for the analysis of lipids and metabolites; (iii) facilities for the cultivation, isolation, and long-term storage of microbial strains; and (iv) curation of a community searchable database containing information about strains isolated from native Hawaiian fauna and ecosystems. The M-GAL core will be available to researchers throughout the state of Hawai'i and visiting scientists, thus encouraging greater interaction amongst the local microbiome research community and seeding collaborative efforts from abroad. The M-GAL Core will beneficially impact COBRE investigators and affiliates by providing essential infrastructure and expertise to advance their research and foster the careers of junior faculty. Moreover, the Core will support the growth of UHM as a major center for microbiome studies in the Pacific Region by providing hands-on training in library preparation and metabolite analysis, hosting an international summer school on fundamental microbiome research methods, and facilitating outreach efforts to characterize Hawai'i's microbiome. !