Request for a ThermoFisher Helios 5UC DualBeam

索取 ThermoFisher Helios 5UC DualBeam

• 批准号: 10719755
• 负责人: ZACHARY FREYBERG
• 金额: $ 60万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10719755
• 关键词: 3-Dimensional; Address; Applications Grants; Autopsy; Behavior; Brain; Brain imaging; Complex; Data; Data Set; Dedications; Electron Microscope; Electron Microscopy; Funding; Human; Individual; Ions; Maps; Mental disorders; Methods; Microscopy; National Institute of Mental Health; Nervous System; Neuropil; Neurosciences Research; Organelles; Research; Research Personnel; Resources; Scanning; Structure; Synapses; System; Technology; Tissue imaging; Universities; brain dysfunction; brain tissue; brain volume; experience; instrument; medical schools; microscopic imaging; nanometer resolution; programs; response; translational neuroscience

7. Project Summary/Abstract: This request is for funds to purchase a ThermoFisher Helios 5UC DualBeam Focused Ion Beam Scanning Electron Microscope (FIB-SEM) system. This instrument is being requested by a group of NIMH-funded investigators who have a significant need to acquire 3-dimensional (3D) volumes of brain tissue imaged at nanometer resolution. The requested FIB-SEM system will be housed in a dedicated facility within the Translational Neuroscience Program (TNP) at the University of Pittsburgh School of Medicine (UPSOM). Installation of the requested Helios 5 UC DualBeam FIB-SEM represents an important expansion of existing shared microscopy facilities that are specialized for, and dedicated to, brain tissue imaging. There is a pressing and prevalent need for 3D large volume electron microscopy (VEM) approaches that permit studies interrogating the basis of brain mechanisms underlying complex behaviors and their disruptions in psychiatric disorders. The advent of 3D volume electron microscopy (VEM) addresses this critical need by generating highly detailed connectomic maps of individually resolved synapses, and the sub-synaptic organelles within, across densely packed, large volumes of brain tissue. The requested FIB-SEM system permits automated and quantitative VEM imaging of brain tissue that avoids many of the limitations associated with other VEM approaches. Although FIB-SEM application to neuroscience research is relatively recent, we show that this technology can be applied to postmortem human brain tissue to create 3D, information-rich datasets of volumes of brain tissue neuropil ready for quantitative analysis. Thus, with this technology we can interrogate the fine structure of the nervous system and pinpoint structural disruptions contributing to brain dysfunction. The need for this instrument is also demonstrated by the overwhelming response from NIMH-funded researchers in the UPSOM. We clearly explain how the requested FIB-SEM will advance the research objectives of current NIMH-funded projects and how this resource is invaluable for generating new NIMH grant applications. The investigators in this proposal have decades of experience in traditional EM approaches, but these methods cannot generate the quantity, or the quality, of data generated by FIB-SEM imaging. Therefore, there is a single prime reason for this application: to provide critically needed, cutting-edge, high quality automated VEM imaging to NIMH supported users within the UPSOM.

7。项目摘要/摘要： 该要求是要购买Thermofisher Helios 5UC双向侧面离子光束扫描 电子显微镜（FIB-SEM）系统。一组NIMH资助 在成像的3维（3D）脑组织中非常需要的研究者 纳米分辨率。请求的FIB-SEM系统将安置在专用设施中 匹兹堡大学医学院（UPSOM）的转化神经科学计划（TNP）。 请求的Helios 5 UC DualBeam Fib-Sem的安装代表了现有的重要扩展 专门用于脑组织成像的共享显微镜设施。 3D大量电子显微镜（VEM）方法有压力和普遍的需求 研究询问了复杂行为的基础的基础 精神疾病。 3D体积电子显微镜（VEM）的出现通过 生成高度详细的连接图，单独分辨突触和突触的细胞器 内部，密集的大量脑组织。请求的FIB-SEM系统允许自动化 和脑组织的定量VEM成像，避免了许多与其他VEM相关的局限性 方法。尽管在神经科学研究中的FIB-SEM应用相对较新，但我们表明这是 技术可以应用于验尸人类脑组织，以创建3D，信息丰富的数据集 脑组织神经纤维的准备进行定量分析。因此，通过这项技术，我们可以审问罚款 神经系统的结构和确定导致脑功能障碍的结构破坏。 NIMH资助的研究人员的压倒性反应也证明了对该工具的需求 在Upsom中。我们清楚地解释了所请求的FIB-SEM将如何提高当前的研究目标 NIMH资助的项目以及该资源对于生成新的NIMH赠款应用程序的宝贵价值。这 该提案中的调查人员在传统方法方面具有数十年的经验，但是这些方法 无法生成FIB-SEM成像产生的数据的数量或质量。因此，有一个 此应用程序的主要原因：提供急需的，最先进的高质量自动化VEM成像 向NIMH支持UPSOM中的用户。