Three-dimensional, Portable, Inexpensive, and Reusable Tomographic Microscopy

三维、便携式、廉价且可重复使用的断层扫描显微镜

基本信息

  • 批准号:
    10721691
  • 负责人:
  • 金额:
    $ 8.85万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2023
  • 资助国家:
    美国
  • 起止时间:
    2023-07-01 至 2025-06-30
  • 项目状态:
    未结题

项目摘要

PROJECT SUMMARY Three-dimensional (3D) microscopy offers many promises for biological investigations and medical applications. However, it is currently limited to well-resourced laboratories in settings with established infrastructure. Many 3D microscopy techniques (e.g., confocal) rely on focusing light at an array of small locations within the sample, which requires expensive and specialized equipment. Optical Projection Tomography (OPT) is a 3D imaging technique that utilizes traditional microscopy equipment; instead of focusing the light at specific locations, OPT images a sample from many angles to reconstruct a 3D volume. It is a very effective method for 3D imaging of small translucent objects (e.g., mouse fetuses, parasites, and large bacteria). While it is possible for OPT to be a lower-cost method of 3D microscopy, existing systems remain expensive and large. We propose to take advantage of the ubiquitous and high-quality computing and imaging hardware available in smartphones to make an OPT device that is inexpensive and extremely portable. We will create a smartphone extension that robustly images a rotating sample and uses the phone’s computational hardware to reconstruct the 3D volume. Two imaging modalities will be pursued: visible-band attenuation microscopy (e.g., brightfield) and luminescent microscopy (e.g., bioluminescent). The components of the smartphone extension are either 3D printed, laser cut acrylic, or readily commercially available. Thus, the cost of manufacturing the device is extremely small and the total weight of the device is very low; the total cost of all components will be less than $50. The components of the extension are easily assembled on site, permitting the device to be transported with in a small package. Due to its low cost and size, the OPT microscope can also serve as a useful tool for educational purposes (e.g., as part of an undergraduate laboratory course involving optics) and for generating real data for tomographic algorithm development. To validate the device, we will build two phantoms with three-dimensional features that allow us to evaluate the Modulation Transfer Function: one for attenuation microscopy and another for luminescent microscopy. Aim 1: Build a visible-band tomographic microscope extension to a smartphone. Aim 1A: Implement 3D cone-beam reconstruction from visible-band sinogram data for samples approximately 10 mm in size with approximately 10 µm resolution. Aim 1B: Improve the image resolution with a multi-lens optical system. Aim 2: Implement bioluminescent tomographic microscopy by appropriately modifying the tomographic reconstruction algorithm. Aim 3: Build two shelf-stable 3D phantoms with features of sizes varying from 1 µm to 200 µm.
项目总结

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Nicholas Dwork其他文献

Nicholas Dwork的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

相似海外基金

Study on the use of 3D print models to improve understanding of geomorphic processes
研究使用 3D 打印模型来提高对地貌过程的理解
  • 批准号:
    22K13777
  • 财政年份:
    2022
  • 资助金额:
    $ 8.85万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
3D print-on-demand technology for personalised medicines at the point of care
用于护理点个性化药物的 3D 按需打印技术
  • 批准号:
    10045111
  • 财政年份:
    2022
  • 资助金额:
    $ 8.85万
  • 项目类别:
    Grant for R&D
Regenerative cooling optimisation in 3D-print rocket nozzles
3D 打印火箭喷嘴的再生冷却优化
  • 批准号:
    2749141
  • 财政年份:
    2022
  • 资助金额:
    $ 8.85万
  • 项目类别:
    Studentship
Development of a New Powder Mix and Process Plan to 3D Print Ductile Iron Parts
开发用于 3D 打印球墨铸铁零件的新粉末混合物和工艺计划
  • 批准号:
    548945-2019
  • 财政年份:
    2021
  • 资助金额:
    $ 8.85万
  • 项目类别:
    College - University Idea to Innovation Grants
Development of a New Powder Mix and Process Plan to 3D Print Ductile Iron Parts
开发用于 3D 打印球墨铸铁零件的新粉末混合物和工艺计划
  • 批准号:
    548945-2019
  • 财政年份:
    2020
  • 资助金额:
    $ 8.85万
  • 项目类别:
    College - University Idea to Innovation Grants
Administrative Supplement for Equipment: 6-axis Positioner to Improve 3D Print Quality and Print Size
设备管理补充:用于提高 3D 打印质量和打印尺寸的 6 轴定位器
  • 批准号:
    10801667
  • 财政年份:
    2019
  • 资助金额:
    $ 8.85万
  • 项目类别:
SBIR Phase II: Pellet based 3D print extrusion process for shoe manufacturing
SBIR 第二阶段:用于制鞋的基于颗粒的 3D 打印挤出工艺
  • 批准号:
    1738138
  • 财政年份:
    2017
  • 资助金额:
    $ 8.85万
  • 项目类别:
    Standard Grant
Development of "artificial muscle' ink for 3D print of microrobots
开发用于微型机器人3D打印的“人造肌肉”墨水
  • 批准号:
    17K18852
  • 财政年份:
    2017
  • 资助金额:
    $ 8.85万
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
I-Corps: Nanochon, a Commercial Venture to 3D Print Regenerative Implants for Joint Reconstruction
I-Corps:Nanochon,一家商业企业,致力于 3D 打印再生植入物进行关节重建
  • 批准号:
    1612567
  • 财政年份:
    2016
  • 资助金额:
    $ 8.85万
  • 项目类别:
    Standard Grant
SBIR Phase I: Pellet based 3D print extrusion process for shoe manufacturing
SBIR 第一阶段:用于制鞋的基于颗粒的 3D 打印挤出工艺
  • 批准号:
    1621732
  • 财政年份:
    2016
  • 资助金额:
    $ 8.85万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了