Three-dimensional, Portable, Inexpensive, and Reusable Tomographic Microscopy

三维、便携式、廉价且可重复使用的断层扫描显微镜

• 批准号: 10721691
• 负责人: Nicholas Dwork
• 金额: $ 8.85万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10721691
• 关键词: 3-Dimensional; 3D Print; Algorithms; Animals; Attenuated; Bacteria; Biological; Biology; Biomedical Engineering; Caenorhabditis elegans; Cellular Phone; Charge; Cone; Coupled; Data; Detection; Devices; Diagnostic; Diameter; Education; Elements; Equipment; Evaluation; Exclusion; Fetus; Funding; Home; Image; Imaging Device; Imaging Techniques; Imaging technology; Infrastructure; Investigation; Laboratories; Lasers; Light; Lighting; Location; Medical; Methods; Microscope; Microscopy; Modernization; Motor; Mus; Optics; Organism; Parasites; Pathology; Performance; Research; Resolution; Resources; Rotation; Rural; Sampling; Shapes; Site; Source; Structure; System; Techniques; Technology; Telephone; Testing; Three-Dimensional Image; Three-Dimensional Imaging; Tissues; Virus Diseases; Visible Radiation; Weight; Work; absorption; algorithm development; attenuation; bioluminescence imaging; cost; imaging modality; improved; insight; laboratory curriculum; lens; light emission; light transmission; lithography; manufacture; manufacturing cost; meter; millimeter; polydimethylsiloxane; portability; reconstruction; remote location; tomography; tool; undergraduate student

PROJECT SUMMARY Three-dimensional (3D) microscopy offers many promises for biological investigations and medical applications. However, it is currently limited to well-resourced laboratories in settings with established infrastructure. Many 3D microscopy techniques (e.g., confocal) rely on focusing light at an array of small locations within the sample, which requires expensive and specialized equipment. Optical Projection Tomography (OPT) is a 3D imaging technique that utilizes traditional microscopy equipment; instead of focusing the light at specific locations, OPT images a sample from many angles to reconstruct a 3D volume. It is a very effective method for 3D imaging of small translucent objects (e.g., mouse fetuses, parasites, and large bacteria). While it is possible for OPT to be a lower-cost method of 3D microscopy, existing systems remain expensive and large. We propose to take advantage of the ubiquitous and high-quality computing and imaging hardware available in smartphones to make an OPT device that is inexpensive and extremely portable. We will create a smartphone extension that robustly images a rotating sample and uses the phone’s computational hardware to reconstruct the 3D volume. Two imaging modalities will be pursued: visible-band attenuation microscopy (e.g., brightfield) and luminescent microscopy (e.g., bioluminescent). The components of the smartphone extension are either 3D printed, laser cut acrylic, or readily commercially available. Thus, the cost of manufacturing the device is extremely small and the total weight of the device is very low; the total cost of all components will be less than $50. The components of the extension are easily assembled on site, permitting the device to be transported with in a small package. Due to its low cost and size, the OPT microscope can also serve as a useful tool for educational purposes (e.g., as part of an undergraduate laboratory course involving optics) and for generating real data for tomographic algorithm development. To validate the device, we will build two phantoms with three-dimensional features that allow us to evaluate the Modulation Transfer Function: one for attenuation microscopy and another for luminescent microscopy. Aim 1: Build a visible-band tomographic microscope extension to a smartphone. Aim 1A: Implement 3D cone-beam reconstruction from visible-band sinogram data for samples approximately 10 mm in size with approximately 10 µm resolution. Aim 1B: Improve the image resolution with a multi-lens optical system. Aim 2: Implement bioluminescent tomographic microscopy by appropriately modifying the tomographic reconstruction algorithm. Aim 3: Build two shelf-stable 3D phantoms with features of sizes varying from 1 µm to 200 µm.

项目概要 三维 (3D) 显微镜为生物研究和医学提供了许多希望 应用程序。然而，目前仅限于资源充足的实验室以及已建立的环境。 基础设施。许多 3D 显微镜技术（例如共焦）依赖于将光聚焦在一系列小 样本内的位置，这需要昂贵且专门的设备。光学投影 断层扫描 (OPT) 是一种利用传统显微镜设备的 3D 成像技术；而不是 OPT 将光聚焦在特定位置，从多个角度对样本进行成像以重建 3D 体积。 对于小型半透明物体（例如小鼠胎儿、寄生虫和昆虫）的 3D 成像来说，这是一种非常有效的方法。 大细菌）。虽然 OPT 可能是一种成本较低的 3D 显微镜方法，但现有系统 仍然昂贵且庞大。我们建议利用无处不在的高质量计算 和智能手机中可用的成像硬件，以制造价格低廉且极其便宜的 OPT 设备 便携的。我们将创建一个智能手机扩展，可以对旋转样本进行稳健成像并使用 手机的计算硬件重建 3D 体积。将采用两种成像方式： 可见光带衰减显微镜（例如明场）和发光显微镜（例如生物发光）。 智能手机扩展的组件要么是 3D 打印、激光切割亚克力，要么是易于商业化的 可用的。因此，该装置的制造成本极低，并且装置的总重量为 非常低；所有组件的总成本将低于 50 美元。扩展的组件很容易 现场组装，允许设备以小包装运输。由于其成本低且 由于尺寸较小，OPT 显微镜还可以作为教育目的的有用工具（例如，作为 涉及光学的本科实验课程）并为断层扫描算法生成真实数据 发展。为了验证该设备，我们将构建两个具有三维特征的模型，允许 我们评估调制传递函数：一个用于衰减显微镜，另一个用于发光 显微镜。目标 1：构建智能手机的可见波段断层扫描显微镜扩展。目标 1A： 根据约 10 mm 的样品的可见波段正弦图数据实施 3D 锥形束重建 尺寸约为 10 µm 分辨率。目标 1B：利用多镜头光学器件提高图像分辨率 系统。目标 2：通过适当修改 断层扫描重建算法。目标 3：构建两个具有尺寸特征的可稳定保存的 3D 模型 范围从 1 µm 到 200 µm。