Viral and Host Factors Promoting Male-to-Female Transmission of HIV

促进艾滋病毒男性向女性传播的病毒和宿主因素

• 批准号: 7684924
• 负责人: Warner C. Greene
• 金额: $ 33.23万
• 依托单位: J. DAVID GLADSTONE INSTITUTES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7684924
• 关键词: Acute; Adult; Alabama; Biological; Biological Assay; Biology; C Type Lectin Receptors; CCR5 gene; CD4 Positive T Lymphocytes; California; Cells; Charge; Cleaved cell; Consult; DNA Sequencing Facility; Dendritic Cells; Dextran Sulfate; Doctor of Philosophy; Dose; Engineering; Enhancers; Environment; Failure; Female; Frequencies; Future; Generations; Genes; Genetic Recombination; Genital system; Genome; HIV; HIV Infections; HIV vaccine; HIV-1; Heparin; Immature Monocyte; Immunology; In Vitro; Infection; Inorganic Sulfates; Institutes; Integration Host Factors; Kinetics; Leadership; Letters; Mediating; Methods; Mucous Membrane; Nature; North Carolina; Nutrient; Peptide Hydrolases; Peptides; Play; Polymers; Principal Investigator; Process; Production; Property; Proteins; Reaction; Recruitment Activity; Rest; Risk; Role; Sampling; San Francisco; Seminal; Seminal Plasma; Seminal fluid; Serum; Site; T memory cell; Techniques; Testing; Tissues; Universities; Unspecified or Sulfate Ion Sulfates; Viral; Viral Load result; Virion; Virus; Virus Diseases; Woman; base; cell type; cellular targeting; cohort; effusion; env Gene Products; env Genes; glycosylation; in vitro Assay; inhibitor/antagonist; insight; macrophage; male; member; men; microbicide; novel strategies; programs; prostatic fraction Acid phosphatase isoenzyme; reproductive; research study; sperm cell; success; transcytosis; transmission process; virology

1 of WHP-San Francisco, serves as Director of the Gladstone Institute of Virology and Immunology. 16. LETTERS OF SUPPORT (e.g., Consultants) Letters of support from the following consults are included on pages 79-83. Beatrice Hahn, MD / George Shaw, MD, PhD (University of Alabama, Birmingham) will provide their technical assistance and advice regarding methods to amplify, sequence and clone the envelope genes from HIV-1 virions from newly infected women and their transmitting male partner identified in the OPTIONS cohort. Specifically, they will assist in single genome amplification, a technique that precludes in vitro recombination during the PCR reaction. Dr. Shaw is a member of the CHAVI Scientific Leadership group and Program Leader of the CHAVI HIV Virus Biology Discovery Team while Dr. Hahn directs the Viral Sequencing Core in the Center for HIV/AIDS Vaccine Immunology (CHAVI). Barton F. Haynes, MD (Duke University, Durham, North Carolina) is the Director of CHAVI and has formally approved our collaborative efforts with Drs. Beatrice Hahn and George Shaw at the University of Alabama. In the future, we may elect to use envelopes isolated during acute infection in CHAVI when matched M->F transmission pairs are formally identified. Support for these studies is indicated in Dr. Haynes letter. Frederick Hecht, MD (University of California, San Francisco) also forms a key collaborator regarding Specific Aim 1. He will provide the serum samples and semen from M^-F transmission pairs from the OPTIONS (acute infection) cohort. He is currently recruiting more transmission pairs identified as part of the OPTIONS

WHP弗朗西斯科的1名，担任格拉德斯通的董事 病毒学和免疫学研究所。 16.支持信（例如，顾问） 以下咨询的支持信见第79-83页。 Beatrice Hahn，MD /乔治肖，MD，PhD（亚拉巴马大学，伯明翰）将提供他们的 关于扩增、测序和克隆包膜基因方法的技术援助和咨询 来自新感染妇女及其传播男性伴侣的HIV-1病毒粒子， OPTIONS队列。具体来说，它们将有助于单基因组扩增，这是一种排除基因组扩增的技术。 在PCR反应期间的体外重组。Shaw博士是CHAVI科学领导层的成员 CHAVI HIV病毒生物学发现团队的团队和项目负责人，而Hahn博士则指导 HIV/AIDS疫苗免疫学中心（Center for HIV/AIDS Vaccine Immunology，CHAVI） 巴顿F. Haynes博士（杜克大学，北卡罗来纳州达勒姆）是CHAVI的主任， 正式批准了我们与比阿特丽斯·哈恩博士和乔治·肖博士的合作。 亚拉巴马。在未来，我们可能会选择在CHAVI急性感染期间使用隔离的包膜， 匹配的M->F传输对被正式标识。这些研究的支持表明在博士。 海恩斯的信 Frederick Hecht医学博士（加州大学旧金山分校弗朗西斯科）也是一个重要的合作者， 具体目标1.他将提供来自墨西哥的M^-F传播对的血清样本和精液。 OPTIONS（急性感染）队列。他目前正在招募更多的传输对， 的选项