权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Histology and Clinical Repository Core

组织学和临床存储库核心

基本信息

批准号：
7675594
负责人：
JOHN W DAY
金额：
$ 19.17万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-01 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7675594
关键词：
Actins Aftercare Animal Model Arts Biochemical Biological Biological Models Biology Biopsy Caring Catchment Area Cell Lineage Cells Child Chloride Channels Clinic Clinical Communities Comparative Study Consent Country Coupled Cultured Cells DNA DNA Databases Databases Development Diagnostic Diagnostic Procedure Diagnostic tests Disease Enrollment Equus caballus Family Fibroblasts Freezing Funding Generations Genetic Genetic Databases Goals Hereditary Disease Histological Techniques Histology Human Image Laboratories Letters Light Medical center Medicine Methods Microscopic Minnesota Modeling Mus Muscle Muscle function Muscular Dystrophies Musculoskeletal Myoblasts Myopathy Myosin ATPase Myotonic Disorders Myotonic Dystrophy National Institute of Arthritis and Musculoskeletal and Skin Diseases Patients Population Proteins RNA Research Research Ethics Committees Research Methodology Research Personnel Research Project Grants Research Support Residual state Resources Sampling Skin Specific qualifier value Specimen Staining method Stains Tissues Universities Update experience induced pluripotent stem cell lymphoblastoid cell line medical specialties member muscular structure novel programs repository tissue/cell culture

项目摘要

Minnesota CCMBM Core B: Muscle Histology and Clinical Repository Core The University of Minnesota Muscular Dystrophy Clinic cares for one of the largest groups of muscular dystrophy patients in the country. Coupled with the comprehensive genetic and histological diagnostic methods developed and maintained by the MDClinic, this population provides a valuable resource for the MDCenter and this NIAMS Core Center for Musculoskeletal Biology and Medicine. As part of its comprehensive clinical diagnostic facility, the MDClinic has established a repository of DNA, fibroblasts, myoblasts, lymphoblastoid cell lines and residual muscle biopsy tissue from subjects with muscle diseases; if specifically supported for research purposes, this Core will provide great value to many currently funded MDCenter research programs, and will be a valuable asset to the greater MD Research Community. Specific Aims of this Core are: 1) Expand the Muscular Dystrophy Database and DNA/Cell Culture/Tissue Repository. Formally establishing this as a research core will facilitate acquisition and distribution of many more samples. 2) To make state-of-the-art histological techniques accessible to fully and complementarity characterize muscle from patients and from animals that model human muscle disease. These methods will allow detailed characterization of diseased muscle as well as comparative studies after treatment in patients and model systems. 3) To provide tissue or cultured cells for detailed analysis of muscle structure and function. MDCenter research on clinically defined specimens will be of value to many basic and translational programs. Specifically, fibroblasts can be studied directly, differentiated into myoblasts for a full range of genetic, biochemical and force generation (Core C) or used to generate IPS cells (Pilot 1) for developmental studies along multiple cell lineages in specified genetic disorder

明尼苏达州CCMBM核心B：肌肉组织学和临床知识库核心明尼苏达大学肌营养不良症诊所照顾最大的肌肉群之一，营养不良的病人。再加上全面的遗传学和组织学诊断由MDClinic开发和维护的方法，这一人群提供了宝贵的资源， MDCenter和NIAMS肌肉骨骼生物学和医学核心中心。在其综合性临床诊断设施，MDClinic已经建立了DNA，成纤维细胞，来自患有肌肉疾病的受试者的成肌细胞、淋巴母细胞样细胞系和残余肌肉活检组织;如果该核心项目专门用于研究目的，将为目前资助的许多项目提供巨大价值。 MDCenter的研究计划，并将成为一个宝贵的资产，以更大的MD研究社区。具体该核心的目标是： 1)扩展肌营养不良症数据库和DNA/细胞培养/组织库。正式将其确立为研究核心，将有助于采集和分发更多样本。 2)使最先进的组织学技术能够充分和互补地表征从病人和模拟人类肌肉疾病的动物身上提取肌肉。这些方法将允许患病肌肉的详细表征以及患者治疗后的比较研究，模型系统 3)为肌肉结构和功能的详细分析提供组织或培养细胞。MDCenter 对临床确定的标本的研究将对许多基础和转化项目具有价值。具体地说，成纤维细胞可以直接研究，分化成成肌细胞进行全方位的遗传，生物化学和力产生（核心C）或用于产生IPS细胞（试点1）用于发育研究特定遗传病中沿着多细胞谱系