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Histology and Clinical Repository Core

组织学和临床存储库核心

基本信息

批准号：
7675594
负责人：
JOHN W DAY
金额：
$ 19.17万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-01 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7675594
关键词：
Actins Aftercare Animal Model Arts Biochemical Biological Biological Models Biology Biopsy Caring Catchment Area Cell Lineage Cells Child Chloride Channels Clinic Clinical Communities Comparative Study Consent Country Coupled Cultured Cells DNA DNA Databases Databases Development Diagnostic Diagnostic Procedure Diagnostic tests Disease Enrollment Equus caballus Family Fibroblasts Freezing Funding Generations Genetic Genetic Databases Goals Hereditary Disease Histological Techniques Histology Human Image Laboratories Letters Light Medical center Medicine Methods Microscopic Minnesota Modeling Mus Muscle Muscle function Muscular Dystrophies Musculoskeletal Myoblasts Myopathy Myosin ATPase Myotonic Disorders Myotonic Dystrophy National Institute of Arthritis and Musculoskeletal and Skin Diseases Patients Population Proteins RNA Research Research Ethics Committees Research Methodology Research Personnel Research Project Grants Research Support Residual state Resources Sampling Skin Specific qualifier value Specimen Staining method Stains Tissues Universities Update experience induced pluripotent stem cell lymphoblastoid cell line medical specialties member muscular structure novel programs repository tissue/cell culture

项目摘要

Minnesota CCMBM Core B: Muscle Histology and Clinical Repository Core The University of Minnesota Muscular Dystrophy Clinic cares for one of the largest groups of muscular dystrophy patients in the country. Coupled with the comprehensive genetic and histological diagnostic methods developed and maintained by the MDClinic, this population provides a valuable resource for the MDCenter and this NIAMS Core Center for Musculoskeletal Biology and Medicine. As part of its comprehensive clinical diagnostic facility, the MDClinic has established a repository of DNA, fibroblasts, myoblasts, lymphoblastoid cell lines and residual muscle biopsy tissue from subjects with muscle diseases; if specifically supported for research purposes, this Core will provide great value to many currently funded MDCenter research programs, and will be a valuable asset to the greater MD Research Community. Specific Aims of this Core are: 1) Expand the Muscular Dystrophy Database and DNA/Cell Culture/Tissue Repository. Formally establishing this as a research core will facilitate acquisition and distribution of many more samples. 2) To make state-of-the-art histological techniques accessible to fully and complementarity characterize muscle from patients and from animals that model human muscle disease. These methods will allow detailed characterization of diseased muscle as well as comparative studies after treatment in patients and model systems. 3) To provide tissue or cultured cells for detailed analysis of muscle structure and function. MDCenter research on clinically defined specimens will be of value to many basic and translational programs. Specifically, fibroblasts can be studied directly, differentiated into myoblasts for a full range of genetic, biochemical and force generation (Core C) or used to generate IPS cells (Pilot 1) for developmental studies along multiple cell lineages in specified genetic disorder

明尼苏达州CCMBM核心B：肌肉组织学和临床资料库核心明尼苏达大学肌营养不良症诊所负责治疗最大的肌肉营养不良人群之一这个国家的营养不良患者。再加上全面的遗传和组织学诊断方法由MDClinic开发和维护，该人群为 MDCenter和这个NIAMS肌肉骨骼生物学和医学核心中心。作为ITS的一部分综合的临床诊断设施，MDClinic建立了DNA、成纤维细胞、肌肉疾病受试者的成肌细胞、淋巴母细胞系和残余肌肉活检组织；该核心专门用于研究目的，将为许多目前资助的项目提供巨大价值 MDCenter研究计划，并将成为更大的MD研究社区的一项宝贵资产。特定的这一核心的目标是： 1)扩展肌肉营养不良数据库和DNA/细胞培养/组织库。正式地将其作为研究核心将有助于获取和分发更多的样本。 2)使最先进的组织学技术能够充分和互补地表征来自病人和模拟人类肌肉疾病的动物的肌肉。这些方法将允许病变肌肉的详细特征以及患者和患者治疗后的比较研究模型系统。 3)为详细分析肌肉结构和功能提供组织或培养细胞。MDCenter 对临床定义的标本的研究将对许多基础和翻译计划具有价值。具体地说，成纤维细胞可以直接研究，分化为成肌细胞进行全方位的遗传，生化和力量生成(核心C)或用于生成用于发育研究的IPS细胞(试点1) 在特定的遗传疾病中沿着多个细胞谱系