Histology and Clinical Repository Core

明尼苏达州CCMBM核心B：肌肉组织学和临床知识库核心 明尼苏达大学肌营养不良症诊所照顾最大的肌肉群之一， 营养不良的病人。再加上全面的遗传学和组织学诊断 由MDClinic开发和维护的方法，这一人群提供了宝贵的资源， MDCenter和NIAMS肌肉骨骼生物学和医学核心中心。在其 综合性临床诊断设施，MDClinic已经建立了DNA，成纤维细胞， 来自患有肌肉疾病的受试者的成肌细胞、淋巴母细胞样细胞系和残余肌肉活检组织;如果 该核心项目专门用于研究目的，将为目前资助的许多项目提供巨大价值。 MDCenter的研究计划，并将成为一个宝贵的资产，以更大的MD研究社区。具体 该核心的目标是： 1)扩展肌营养不良症数据库和DNA/细胞培养/组织库。正式 将其确立为研究核心，将有助于采集和分发更多样本。 2)使最先进的组织学技术能够充分和互补地表征 从病人和模拟人类肌肉疾病的动物身上提取肌肉。这些方法将允许 患病肌肉的详细表征以及患者治疗后的比较研究， 模型系统 3)为肌肉结构和功能的详细分析提供组织或培养细胞。MDCenter 对临床确定的标本的研究将对许多基础和转化项目具有价值。 具体地说，成纤维细胞可以直接研究，分化成成肌细胞进行全方位的遗传， 生物化学和力产生（核心C）或用于产生IPS细胞（试点1）用于发育研究 特定遗传性疾病中的沿着多个细胞谱系

Minnesota CCMBM Core B: Muscle Histology and Clinical Repository Core The University of Minnesota Muscular Dystrophy Clinic cares for one of the largest groups of muscular dystrophy patients in the country. Coupled with the comprehensive genetic and histological diagnostic methods developed and maintained by the MDClinic, this population provides a valuable resource for the MDCenter and this NIAMS Core Center for Musculoskeletal Biology and Medicine. As part of its comprehensive clinical diagnostic facility, the MDClinic has established a repository of DNA, fibroblasts, myoblasts, lymphoblastoid cell lines and residual muscle biopsy tissue from subjects with muscle diseases; if specifically supported for research purposes, this Core will provide great value to many currently funded MDCenter research programs, and will be a valuable asset to the greater MD Research Community. Specific Aims of this Core are: 1) Expand the Muscular Dystrophy Database and DNA/Cell Culture/Tissue Repository. Formally establishing this as a research core will facilitate acquisition and distribution of many more samples. 2) To make state-of-the-art histological techniques accessible to fully and complementarity characterize muscle from patients and from animals that model human muscle disease. These methods will allow detailed characterization of diseased muscle as well as comparative studies after treatment in patients and model systems. 3) To provide tissue or cultured cells for detailed analysis of muscle structure and function. MDCenter research on clinically defined specimens will be of value to many basic and translational programs. Specifically, fibroblasts can be studied directly, differentiated into myoblasts for a full range of genetic, biochemical and force generation (Core C) or used to generate IPS cells (Pilot 1) for developmental studies along multiple cell lineages in specified genetic disorder