Mechanisms of Adaptation to Exercise in Health and COPD

健康和慢性阻塞性肺病的运动适应机制

• 批准号: 7561197
• 负责人: PETER D WAGNER
• 金额: $ 205.12万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-08 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7561197
• 关键词: Mechanisms; Adaptation; Exercise; Health; COPD

DESCRIPTION (provided by applicant): The objective of this multidisciplinary proposal is to understand key mechanisms of muscle adaptation to exercise in health and, especially, in COPD. Exercise capacity is impaired in COPD, and increasingly skeletal muscle and cardiovascular dysfunction are implicated. Increased oxidative stress and reduced muscle capillarity have been reported and will be a major theme in this application. Studies will be performed in humans with COPD, in mouse muscle single fibers and in several intact transgenic mouse lines to address mechanisms by which hypoxia and oxidative stress affect muscle structure, function and responses to exercise in health and in COPD. In humans, the sources and importance of oxidative stress will be studied in COPD patients with normal and reduced lean body mass (cachectic phenotype). In mice, the possible roles of oxidative stress, inflammation and apoptosis in the exercise response will be investigated using four specially created transgenic lines. Two of these produce an emphysematous phenotype with muscle wasting based on a) inflammation (pulmonary TNF-a overexpression) and b) apoptosis (pulmonary VEGF deletion). The other two deplete VEGF in c) heart and d) skeletal muscle, which is relevant because reduced muscle VEGF levels are found in COPD. Studies will be performed in both isolated single muscle fibers and intact animals. This research program will elucidate the effects of lung damage in COPD on skeletal muscle, and also will address interactions among potential comorbid conditions common in COPD - cardiac and muscle dysfunction. Overarching hypotheses are that while muscle adaptive responses may be in part signaled by oxidative stress, excessive oxidative stress interferes with muscle contractile and vascular function and also the expression of genes important in adaptation, especially VEGF. Project 1 (Wagner) uses muscle biopsy samples from COPD patients studied in Project 3 to assess the roles of inflammation and oxidative stress on muscle function and VEGF expression and action. It also studies the above four transgenic lines to explore possible pathways to muscle dysfunction in COPD and to understand interactions among impaired lungs, skeletal muscle and heart. Project 2 (Hogan) uses mouse single muscle fibers to elucidate the mechanisms by which hypoxia and oxidative stress impair muscle contractile function, using both normal mice and the same transgenic line overexpressing TNF-a in the lung as Project 1. Project 3 (Richardson) examines sources and importance of oxidative stress to acute exercise and to training in patients with COPD. The program is supported by tissue imaging and administrative cores. Elucidating mechanisms of adaptive response to exercise - in particular the role of oxidative stress - and the mechanisms relating lung disease to muscle dysfunction, should lead to specific, rational strategies for improving exercise capacity and quality of life in patients with COPD.

描述（由申请人提供）： 这个多学科的建议的目的是了解肌肉适应运动的健康，特别是在COPD的关键机制。COPD患者的运动能力受损，并且越来越多地涉及骨骼肌和心血管功能障碍。增加氧化应激和减少肌肉毛细作用已被报道，并将在此应用的一个主要主题。将在患有COPD的人类、小鼠肌肉单纤维和几种完整的转基因小鼠系中进行研究，以解决缺氧和氧化应激影响健康和COPD中肌肉结构、功能和运动反应的机制。在人类中，将在瘦体重正常和减少的COPD患者（恶病质表型）中研究氧化应激的来源和重要性。在小鼠中，氧化应激，炎症和细胞凋亡在运动反应中的可能作用将使用四个专门创建的转基因系进行研究。其中两种基于a）炎症（肺TNF-α过表达）和B）凋亡（肺VEGF缺失）产生具有肌肉萎缩的肺气肿表型。另外两种消耗c）心脏和d）骨骼肌中的VEGF，这是相关的，因为在COPD中发现肌肉VEGF水平降低。研究将在分离的单个肌纤维和完整动物中进行。该研究计划将阐明COPD肺损伤对骨骼肌的影响，并将解决COPD常见的潜在共病-心脏和肌肉功能障碍之间的相互作用。最重要的假设是，虽然肌肉适应性反应可能部分由氧化应激发出信号，但过度的氧化应激干扰肌肉收缩和血管功能，以及在适应中重要的基因的表达，特别是VEGF。项目1（瓦格纳）使用项目3中研究的COPD患者的肌肉活检样本来评估炎症和氧化应激对肌肉功能以及VEGF表达和作用的作用。本研究还研究了上述四种转基因品系，以探索COPD肌肉功能障碍的可能途径，并了解受损肺、骨骼肌和心脏之间的相互作用。项目2（Hogan）使用正常小鼠和与项目1相同的在肺中过表达TNF-α的转基因系，使用小鼠单根肌纤维来阐明缺氧和氧化应激损害肌肉收缩功能的机制。项目3（Richardson）研究了COPD患者急性运动和训练中氧化应激的来源和重要性。该计划由组织成像和管理核心支持。阐明运动适应性反应的机制--特别是氧化应激的作用--以及肺部疾病与肌肉功能障碍的相关机制，应能为改善COPD患者的运动能力和生活质量提供具体、合理的策略。