Biomarker Core
Biomarker Core
批准号:
7613238
负责人:
KAREN L TEFF
金额:
$28.9万
依托单位国家:
美国
项目类别:
财政年份:
2008
资助国家:
美国
项目状态:
已结题
起止时间:
2008-12-01 至 2013-11-30
关键词:
AminesArtsBiochemistryBiological AssayBiological MarkersBloodBlood specimenCell Adhesion MoleculesCenter for Translational Science ActivitiesChemistryClinicalClinical ResearchClinical and Translational Science AwardsCodeCollectionCore FacilityCoupledDetectionDiabetes MellitusEndocrinologyEnsureEnzyme-Linked Immunosorbent AssayEquipmentEragrostisFundingGene ExpressionGlucoseHigh Pressure Liquid ChromatographyHormonesHousingHumanIcelandInflammatoryInsulinIsoprostanesLaboratoriesLaboratory PersonnelLeadershipLeptinLettersMeasurementMeasuresMelatoninMolecularMolecular BiologyMonitorNeurobiologyNonesterified Fatty AcidsNorepinephrineObesityOxidative StressPennsylvaniaPlasmaPolymerase Chain ReactionProcessProteinsQuality ControlRadioimmunoassayRattusResearchResearch PersonnelResourcesReverse TranscriptionRoleSamplingSavingsSiteSleepSleep Apnea SyndromesSpecimen HandlingSystemSystems BiologyTimeTissuesTongueTranslational ResearchTriglyceridesUniversitiesUrineadiponectincostcytokinedata sharingdetectorendophenotypeexperiencehuman subjectinstrumentationmonocyteprofessorrespiratorysquare footurinary
中文摘要
生物标志物核心将主要位于转化研究实验室(TRL),同样
英文摘要
The Biomarker Core will be primarily located in the Translational Research Laboratory (TRL), the same
building which houses the Center for Sleep and Respiratory Neurobiology. The formation of the Biomarker
Core will take advantage of existing facilities and the sophisticated equipment already available within the
Translational Research Laboratory. The Biomarker Core will act as a central facility to collect, process and
analyze the large number of incoming blood and urine samples generated by the clinical Projects 02 and 03
as well as rat tissue samples and isolated human monocytes derived from Projects 01 and 03. Importantly,
the Biomarker Core will ensure quality control of all samples collected by the proposed projects. The
objectives of the Biomarker Core are to: 1) standardize blood and urine collection between sites, 2) ensure
proper handling and storage of samples, 3) code, record and monitor incoming samples 4) ensure quality
control of assays and 5) provide a centralized facility for assays that are not available at other facilities within
the institute. The Biomarker Core will be under the leadership of Dr. Karen Teff with Dr. Miroslaw
Mackiewicz as Co-Core Leader and support staff consisting of a senior research technician and minimal
effort from an additional technician in Dr. Teff's laboratory. Biomarkers will fall into the following categories:
markers of oxidation and protective anti-oxidants, pro-inflammatory and anti-inflammatory markers,
adhesion molecules, indices of insulin resistance and indices of sympathetic nervous system activity. The
Biomarker Core will conduct three types of assays: 1) high-pressure liquid chromatography (HPLC) for
measurement of plasma and urinary norepinephrine and metabolites such as normetanephrine as well as
melatonin, a specific sleep-related biomarker, 2) spectrophotometric methods for the analysis of free fatty
acids and triglycerides and 3) Reverse Transcription - Polymerase Chain Reaction (RT-PCR) on rat tongue
tissue and isolated human monocytes. To avoid duplication of resources, we will take advantages of other
cores at Penn. The Clinical and Translational Research Center Laboratory routinely conducts Enzyme
Linked Immunosorbent Assay (ELISA) for the measurement of the inflammatory cytokines (IL-6, TNF-a), the
anti-inflammatory cytokine (IL-10) and adhesion molecules (ICAM-1). In addition, markers of insulin
resistance and sensitivity requiring radioimmunoassays (insulin, leptin, adiponectin) will be measured by the
Penn Diabetes Research Center Radioimmunoassay Core. Urinary isoprostanes will be measured in the
laboratory of Dr. Ian Blair, Professor of Chemistry and a renowned expert in markers of oxidative stress.
Plasma glucose will be analyzed in the laboratory of Dr. Teff. A centralized facility will ensure quality control
and cost effectiveness and will support future projects by establishing a bank of samples that will be
available for future investigation.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Sweet taste receptors and glucose metabolism in healthy and T2DM humans
-
批准号:8189922
-
项目类别:
-
资助金额:$20.75万
-
财政年份:2011
-
负责人:KAREN L TEFF
-
依托单位:
Sweet taste receptors and glucose metabolism in healthy and T2DM humans
-
批准号:8313879
-
项目类别:
-
资助金额:$18.42万
-
财政年份:2011
-
负责人:KAREN L TEFF
-
依托单位:
Atypical Antipsychotics: Effects on Hepatic Glucose and Lipid Metabolism in Human
-
批准号:7714499
-
项目类别:
-
资助金额:$49.54万
-
财政年份:2009
-
负责人:KAREN L TEFF
-
依托单位:
Atypical Antipsychotics: Effects on Hepatic Glucose and Lipid Metabolism in Human
-
批准号:8101027
-
项目类别:
-
资助金额:$43.48万
-
财政年份:2009
-
负责人:KAREN L TEFF
-
依托单位:
Atypical Antipsychotics: Effects on Hepatic Glucose and Lipid Metabolism in Human
-
批准号:7924528
-
项目类别:
-
资助金额:$47.21万
-
财政年份:2009
-
负责人:KAREN L TEFF
-
依托单位:
Atypical Antipsychotics: Effects on Hepatic Glucose and Lipid Metabolism in Human
-
批准号:8288242
-
项目类别:
-
资助金额:$29.02万
-
财政年份:2009
-
负责人:KAREN L TEFF
-
依托单位:
The Cholinergic Anti-Inflammatory Pathway in Human Obesity and Insulin Resistance
-
批准号:7578312
-
项目类别:
-
资助金额:$18.52万
-
财政年份:2008
-
负责人:KAREN L TEFF
-
依托单位:
The Cholinergic Anti-Inflammatory Pathway in Human Obesity and Insulin Resistance
-
批准号:7470213
-
项目类别:
-
资助金额:$20.79万
-
财政年份:2008
-
负责人:KAREN L TEFF
-
依托单位:
EFFECT OF PROLONGED MILD HYPERGLYCEMIA ON THE NEURAL
-
批准号:7199055
-
项目类别:
-
资助金额:$0.72万
-
财政年份:2004
-
负责人:KAREN L TEFF
-
依托单位:
EFFECT OF HYPERGLYCEMIA ON AUTONOMIC ACTIVITY
-
批准号:7199023
-
项目类别:
-
资助金额:$0.31万
-
财政年份:2004
-
负责人:KAREN L TEFF
-
依托单位:
MENSTRUAL CYCLE EFFECTS ON INSULIN SENSITIVITY IN TYPE 1 DIABETIC WOMEN
-
批准号:7199058
-
项目类别:
-
资助金额:$3.31万
-
财政年份:2004
-
负责人:KAREN L TEFF
-
依托单位:
EFFECT OF FRUCTOSE AND GLUCOSE ON LEPTIN, GHRELIN AND TRIGLYCERIDES
-
批准号:7199059
-
项目类别:
-
资助金额:$3.31万
-
财政年份:2004
-
负责人:KAREN L TEFF
-
依托单位:
Effect of Fructose and Glucose on Leptin, Ghrelin and Triglycerides
-
批准号:7039611
-
项目类别:
-
资助金额:$5.36万
-
财政年份:2003
-
负责人:KAREN L TEFF
-
依托单位:
Effect of Hyperglycemia on Autonomic Activity
-
批准号:7039566
-
项目类别:
-
资助金额:$2.91万
-
财政年份:2003
-
负责人:KAREN L TEFF
-
依托单位:
Prolonged Mild Hyperglycemia on the Neural Contribution to Insulin & Degradation
-
批准号:7039606
-
项目类别:
-
资助金额:$8.24万
-
财政年份:2003
-
负责人:KAREN L TEFF
-
依托单位:
Menstrual Cycle Effects on Insulin Sensitivity in Type 1 Diabetic Women
-
批准号:7039610
-
项目类别:
-
资助金额:$1.38万
-
财政年份:2003
-
负责人:KAREN L TEFF
-
依托单位:
INHIBITION OF PARASYMPATHETIC ACTIVITY--EFFECT ON INSULIN RELEASE
-
批准号:6565912
-
项目类别:
-
资助金额:$12.41万
-
财政年份:2001
-
负责人:KAREN L TEFF
-
依托单位:
VAGALLY MEDIATED INSULIN RELEASE AFTER PROLONGED HYPERGLYCEMIA
-
批准号:6565802
-
项目类别:
-
资助金额:$12.41万
-
财政年份:2001
-
负责人:KAREN L TEFF
-
依托单位:
INHIBITION OF PARASYMPATHETIC ACTIVITY--EFFECT ON INSULIN RELEASE
-
批准号:6468162
-
项目类别:
-
资助金额:$12.41万
-
财政年份:2000
-
负责人:KAREN L TEFF
-
依托单位:
VAGALLY MEDIATED INSULIN RELEASE AFTER PROLONGED HYPERGLYCEMIA
-
批准号:6468052
-
项目类别:
-
资助金额:$12.41万
-
财政年份:2000
-
负责人:KAREN L TEFF
-
依托单位:
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