Molecular Biology of Drosophila Learning and Memory

果蝇学习和记忆的分子生物学

• 批准号: 7537165
• 负责人: Ronald L Davis
• 金额: $ 33.58万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7537165
• 关键词: Address; Adult; Alleles; Alzheimer' s Disease; Area; Autistic Disorder; Behavioral; Biological Assay; Brain; Calcium; Cells; Cyclic AMP; Drosophila genus; Functional Imaging; Genetic; Grant; Learning; Learning Disabilities; Lobe; Mediating; Memory; Molecular Biology; Molecular and Cellular Biology; Mushroom Bodies; Names; Neurons; Pathway Analysis; Pathway interactions; Phenotype; Process; Proteins; Research; Schizophrenia; Synapses; Synaptic Transmission; Techniques; Testing; Time; cellular imaging; fly; mRNA Expression; member; mutant; nervous system disorder; new technology; overexpression; protein expression; research study; tool; transgene expression

DESCRIPTION (provided by applicant): The long-term goals of this research project are to dissect the molecular and cellular biology of olfactory memory formation in Drosophila. A recent screen for new memory mutants with putative functions in the mushroom body neurons has uncovered several putative mutants, but one with a strong effect on performance immediately after training. Experiments are proposed to completely characterize the nature of the disrupted expression in different alleles of this gene, to reveal the spatial expression pattern of the gene within the olfactory nervous system, and to probe behaviorally whether the various alleles disrupt acquisition or memory stability. Genetic tests will reveal whether the mutant disrupts a molecule that is a member of well characterized signaling systems important for memory formation or whether this new gene represents the prototypic member of a parallel signaling system. Newly developed techniques to control transgene expression in time and in space will be used to address the issue of when and where the gene product is required for normal memory formation. Overall, the experiments will contribute to our understanding of the molecular biology of learning, an important process disrupted in many different diseases of the nervous system including Alzheimer's disease, schizophrenia, autism, and learning disabilities.

描述（由申请人提供）：本研究项目的长期目标是剖析果蝇嗅觉记忆形成的分子和细胞生物学。最近对蘑菇体神经元中具有推定功能的新记忆突变体进行了筛选，发现了几种推定的突变体，但其中一种对训练后立即的表现有强烈影响。实验提出完全表征该基因的不同等位基因中的破坏表达的性质，以揭示嗅觉神经系统内的基因的空间表达模式，并从行为上探测各种等位基因是否破坏了采集或记忆稳定性。基因测试将揭示突变体是否破坏了一个分子，该分子是一个对记忆形成很重要的信号系统的成员，或者这个新基因是否代表了一个平行信号系统的原型成员。新开发的技术，以控制转基因表达的时间和空间将被用来解决的问题，何时何地的基因产物是需要正常的记忆形成。总的来说，这些实验将有助于我们理解学习的分子生物学，这是一个重要的过程，在许多不同的神经系统疾病中被破坏，包括阿尔茨海默病，精神分裂症，自闭症和学习障碍。