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Metabolic targeting of heterogenous myelodysplastic syndrome stem cells

异源性骨髓增生异常综合征干细胞的代谢靶向

基本信息

批准号：
10788983
负责人：
Eric M Pietras
金额：
$ 42.24万
依托单位：
UNIVERSITY OF COLORADO DENVER
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-19 至 2028-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10788983
关键词：
Address Aftercare Automobile Driving Azacitidine Behavior Biological Biological Assay Cells Cellular Indexing of Transcriptomes and Epitopes by Sequencing Clinical Clinical Trials Clone Cells Coupled Data Disease Disease Progression Dose Limiting Dysmyelopoietic Syndromes Foundations Genotype Goals Hematopoietic stem cells Heterogeneity Hydrogenase In complete remission Individual Laboratories Laboratory Study Link Metabolic Modality Modeling Molecular Molecular Genetics Myelogenous Niacinamide Outcome Pathway interactions Patient-Focused Outcomes Patients Pharmaceutical Preparations Phase Proliferating Property Protein Biosynthesis Protein Synthesis Inhibition Protein Synthesis Inhibitors Proteins Recurrent disease Regimen Research Personnel Residual Neoplasm Resistance Resolution STAT3 gene Safety Signal Pathway Specimen Therapeutic Toxic effect Translational Repression Translations Vertebral column Work cancer stem cell cohort design genetic approach high risk improved improved outcome in vivo inhibitor mortality next generation novel strategies novel therapeutic intervention novel therapeutics patient subsets pharmacologic rational design response standard of care stem cell population stem cells treatment response

项目摘要

PROJECT SUMMARY/ABSTRACT The goal of this project is to define vulnerabilities that can be leveraged to comprehensively eradicate MDS stem cells (MDS-SCs). This objective is based on the concept that MDS-SCs lie at the root of disease and are not effectively targeted by current therapies. A significant challenge in achieving this goal is the heterogeneity of the MDS-SC population, as recent studies have identified distinct subsets of MDS-SC with varying responsiveness to therapy. Thus, the premise of our work is that understanding and targeting the unique properties of heterogenous MDS-SCs is critical to improve outcomes in MDS. The foundation of our project is built upon laboratory studies coupled with an active clinical trial (NCT03564873) evaluating the clinical impact of targeting protein synthesis with the global translation inhibitor omacetaxine (oma) in MDS-SC from individuals with high- risk MDS. While the results from our trial using oma in combination with the hypomethylating agent azacytidine (aza) are indeed promising and represent a substantial improvement over standard of care (aza alone), instances of disease progression in patients suggest oma may not eradicate all MDS-SC subsets. These outcomes, alongside toxicities associated with global protein translation inhibition have motivated us to better characterize the unique biological and functional properties of heterogenous MDS-SC subsets. With the goal of developing next-generation approaches that more effectively target MDS-SC while minimizing dose-limiting toxicities to patients, here we will address two primary questions: 1) what is the overall efficacy of protein synthesis inhibition amongst varying MDS-SC subtypes, 2) what are the optimal therapeutic strategies to eradicate MDS-SC based on their sensitivity to protein synthesis inhibition? Hence, we will leverage comprehensive CITE-seq based analyses of serial pre- and post-treatment specimens from our oma/aza clinical trial alongside PDX-based functional studies to identify the link(s) between features such as protein synthesis activity, MDS-SC genotype and clonal behavior with oma response at single cell resolution. We will define the mechanisms governing reliance on protein synthesis in MDS-SC, focusing on pharmacological and molecular genetic-based targeting of key pathways linked with oma sensitivity. Lastly, we will develop improved approaches to comprehensively target heterogenous MDS-SC populations, with focus on next-generation strategies that more selectively target aberrant metabolic features of MDS-SC uncovered by our ongoing CITE-seq characterization of patient specimens. Taken together, the proposed studies will use advanced functional models and single-cell resolution analyses to identify key vulnerabilities of MDS-SC that can be leveraged to design rational therapeutic approaches to improve outcomes for MDS patients.

项目总结/文摘