Optimizing Environmental Enrichment to Model Preclinical Neurorehabilitation

优化环境富集以模拟临床前神经康复

• 批准号: 10789355
• 负责人: ANTHONY E. KLINE
• 金额: $ 3.05万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10789355
• 关键词: Acceleration; Address; Administrative Supplement; Adult; Affect; Affective; Age; Amantadine; Childhood; Clinical; Cognitive; Cognitive deficits; Doctor of Philosophy; Financial Support; Funding; Goals; Grant; Hispanic; Histologic; Individual; Laboratories; Latino; Medical; Mentors; Methods; Modeling; Motor; Music; Neurosciences Research; Outcome; Pediatrics; Pharmacotherapy; Pre-Clinical Model; Rattus; Recovery; Rehabilitation therapy; Research; STEM field; Survivors; Swimming; Therapeutic; Training; Traumatic Brain Injury; Writing; Zaleplon; age group; cognitive recovery; design; environmental enrichment for laboratory animals; graduate school; improved; male; motor deficit; neurological rehabilitation; parent grant; professor; skill acquisition; success; undergraduate student; volunteer

This administrative supplement request is for an increase in funds allotted to my R01 grant (5R01NS084967) to financially support Andrew Victoria, a Latino undergraduate student currently volunteering in my laboratory, as he gains additional training in neuroscience research, which will help him become more competitive for medical/graduate school. Briefly, the goals of the parent grant were to continue to refine environmental enrichment (EE), which is a non-invasive paradigm that promotes significant cognitive recovery and histological protection after experimental traumatic brain injury (TBI) and has the potential to mimic post-TBI clinical rehabilitation. The purpose is significant because TBI affects more than 10 million individuals worldwide each year and results in long-term motor and cognitive deficits, but pharmacotherapies alone have been unsuccessful. Specifically, the parent grant consists of five specific aims that are logical and crucial extensions of our previous research. Aim 1a is designed to determine whether motor, cognitive, and affective benefits can be sustained after EE is withdrawn, and if so, for how long. Aim 1b will determine if providing “refresher rehab” after the EE-induced benefits begin to wane will stabilize or re-strengthen benefits. Aims 2abc will determine whether “bridging” delayed EE, which is initiated at 7-days after TBI, with a) amantadine {10 mg/kg/day; i.p.}, b) aqua therapy {two 90 s swim sessions}, or c) music exposure (3 h per night of New Age, Ambient, or Classical - Mozart’s sonata for two pianos, K.448) as adjunct therapies during the week after TBI will augment recovery relative to non-enriched or Rehab groups, and Aim 3 will evaluate mechanisms for the bridge plus Rehab therapies. Completion of the aims will further advance a model of neurorehabilitation that mimics the real-world while addressing questions that continue to concern physiatrists, such as how long do the rehab benefits last once discontinued and can they be maintained or improved further with supplemental rehab? Can supplemental therapies before full rehab provide a better outcome? The refined model will significantly impact and advance rehabilitation-based research. The administrative supplement will afford Mr. Victoria the opportunity to continue conducting research in a well-established TBI laboratory and expand his research and professional development skills. The project that Andrew will conduct derives from Aim 2a of the parent grant, which seeks to determine whether a bridge therapy (amantadine before the initiation of EE) is more effective than amantadine or EE alone, but differs in the age group (pediatric vs. adult) being treated. Thus, the difference from the parent grant is that here the trainee will evaluate pediatrics rats, but the project is still within the scope as the same question is being asked, and answered, and the same hypothesis and methods are included. Our request will accelerate scientific research and promote diversity in the research and medical workforce. As a Hispanic male, professor, and mentor, I am committed to Andrew’s success in achieving his long-term goal of attaining his MD/PhD as well as promoting diversity in the STEM fields.

此行政补充申请是为了增加分配给我的R 01补助金（5 R 01 NS 084967）的资金 来资助安德鲁维多利亚，一个拉丁裔的本科生，现在在我的实验室做志愿者， 因为他在神经科学研究方面获得了额外的培训，这将有助于他在竞争中更具竞争力。 医学院/研究生院。简而言之，母公司赠款的目标是继续完善环境保护， 丰富（EE），这是一种非侵入性的范式，促进显着的认知恢复， 实验性创伤性脑损伤（TBI）后的组织学保护，并有可能模拟TBI后 临床康复目的是重要的，因为TBI影响全球1000多万人 并导致长期的运动和认知缺陷，但单独的药物治疗已经 不成功。具体而言，父母补助金包括五个具体目标，这些目标是逻辑和关键的延伸 我们以前的研究。目标1a旨在确定运动、认知和情感益处是否可以 如果是的话，可以持续多久。目标1b将决定是否提供“复习康复” 在EE引起的效益开始减弱之后，将稳定或重新加强效益。目标2abc将决定 是否“桥接”延迟EE，其在TBI后7天开始，用a）金刚烷胺{10 mg/kg/天; i. p.}，B） 水疗法{两个90年代的游泳课程}，或c）音乐接触（每晚3小时的新时代，环境，或古典 - 莫扎特的双钢琴奏鸣曲，K.448）作为TBI后一周内的辅助治疗将促进恢复 目标3将评估桥梁加康复的机制 治疗这些目标的实现将进一步推进模拟现实世界的神经康复模型 在解决理疗师继续关注的问题时， 一旦停药，它们是否可以通过补充康复得到维持或进一步改善？可以 在完全康复之前进行补充治疗会有更好的效果吗改进后的模型将显著影响 并推进基于康复的研究行政补助将使维多利亚先生 有机会继续在一个完善的TBI实验室进行研究，并扩大他的研究和 专业发展技能。安德鲁将进行的项目来自母基金的目标2a， 该研究旨在确定过渡疗法（在EE开始前使用金刚烷胺）是否更有效 比单独的金刚烷胺或EE，但不同的年龄组（儿童与成人）正在接受治疗。因此 与父母补助金的不同之处在于，在这里，受训者将评估儿科大鼠，但该项目仍在 同样的问题被问到，回答，同样的假设和方法， 包括.我们的要求将加速科学研究，促进研究和医疗的多样性。 劳动力作为一个西班牙裔男性，教授，导师，我致力于安德鲁的成功，实现他的 实现他的MD/PhD以及促进STEM领域的多样性的长期目标。